Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads
Summary In 2015, there were an estimated 10.4 million new tuberculosis (TB) cases and 1.4 million deaths worldwide. Bacille Calmette–Guérin (BCG), an attenuated strain of Mycobacterium bovis, is the vaccine available against TB, but it is insufficient for global TB control. This study evaluated the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-11-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.12754 |
| _version_ | 1811203783981531136 |
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| author | Patricia Rubio‐Reyes Natalie A. Parlane Bryce M. Buddle D. Neil Wedlock Bernd H. A. Rehm |
| author_facet | Patricia Rubio‐Reyes Natalie A. Parlane Bryce M. Buddle D. Neil Wedlock Bernd H. A. Rehm |
| author_sort | Patricia Rubio‐Reyes |
| collection | DOAJ |
| description | Summary In 2015, there were an estimated 10.4 million new tuberculosis (TB) cases and 1.4 million deaths worldwide. Bacille Calmette–Guérin (BCG), an attenuated strain of Mycobacterium bovis, is the vaccine available against TB, but it is insufficient for global TB control. This study evaluated the immunogenicity of the Mycobacterium tuberculosis antigen Rv1626 in mice while assessing the effect of co‐delivering either Cpe30 (immunostimulatory peptide), CS.T3378–395 (promiscuous T helper epitope) or flagellin (TLR5 agonist) or a combination of all three immunostimulatory agents. Rv1626 and the respective immunostimulatory proteins/peptides were co‐displayed on polyhydroxybutyrate beads assembled inside an engineered endotoxin‐free mutant of Escherichia coli. Mice vaccinated with these beads produced immune responses biased towards Th1‐/Th17‐type responses, but inclusion of Cpe30, CS.T3378–395 and flagellin did not enhance immunogenicity of the Rv1626 protein. This was confirmed in a M. bovis challenge experiment in mice, where Rv1626 beads reduced bacterial cell counts in the lungs by 0.48 log10 compared with the adjuvant alone control group. Co‐delivery of immunostimulatory peptides did not further enhance protective immunity. |
| first_indexed | 2024-04-12T03:02:06Z |
| format | Article |
| id | doaj.art-ac661e0d39f2433887afcf302b9ac013 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-12T03:02:06Z |
| publishDate | 2017-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-ac661e0d39f2433887afcf302b9ac0132022-12-22T03:50:39ZengWileyMicrobial Biotechnology1751-79152017-11-011061434144010.1111/1751-7915.12754Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beadsPatricia Rubio‐Reyes0Natalie A. Parlane1Bryce M. Buddle2D. Neil Wedlock3Bernd H. A. Rehm4Institute of Fundamental Sciences Massey University 11222 Private Bag Palmerston North New ZealandAgResearch, Hopkirk Research Institute Grasslands Research Centre 11008 Private Bag Palmerston North New ZealandAgResearch, Hopkirk Research Institute Grasslands Research Centre 11008 Private Bag Palmerston North New ZealandAgResearch, Hopkirk Research Institute Grasslands Research Centre 11008 Private Bag Palmerston North New ZealandInstitute of Fundamental Sciences Massey University 11222 Private Bag Palmerston North New ZealandSummary In 2015, there were an estimated 10.4 million new tuberculosis (TB) cases and 1.4 million deaths worldwide. Bacille Calmette–Guérin (BCG), an attenuated strain of Mycobacterium bovis, is the vaccine available against TB, but it is insufficient for global TB control. This study evaluated the immunogenicity of the Mycobacterium tuberculosis antigen Rv1626 in mice while assessing the effect of co‐delivering either Cpe30 (immunostimulatory peptide), CS.T3378–395 (promiscuous T helper epitope) or flagellin (TLR5 agonist) or a combination of all three immunostimulatory agents. Rv1626 and the respective immunostimulatory proteins/peptides were co‐displayed on polyhydroxybutyrate beads assembled inside an engineered endotoxin‐free mutant of Escherichia coli. Mice vaccinated with these beads produced immune responses biased towards Th1‐/Th17‐type responses, but inclusion of Cpe30, CS.T3378–395 and flagellin did not enhance immunogenicity of the Rv1626 protein. This was confirmed in a M. bovis challenge experiment in mice, where Rv1626 beads reduced bacterial cell counts in the lungs by 0.48 log10 compared with the adjuvant alone control group. Co‐delivery of immunostimulatory peptides did not further enhance protective immunity.https://doi.org/10.1111/1751-7915.12754 |
| spellingShingle | Patricia Rubio‐Reyes Natalie A. Parlane Bryce M. Buddle D. Neil Wedlock Bernd H. A. Rehm Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads Microbial Biotechnology |
| title | Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads |
| title_full | Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads |
| title_fullStr | Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads |
| title_full_unstemmed | Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads |
| title_short | Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads |
| title_sort | immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads |
| url | https://doi.org/10.1111/1751-7915.12754 |
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