Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome.
Trypanosoma cruzi parasites are the causative agents of Chagas disease. These parasites infect cardiac and gastrointestinal tissues, leading to local inflammation and tissue damage. Digestive Chagas disease is associated with perturbations in food absorption, intestinal traffic and defecation. Howev...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-03-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC5864088?pdf=render |
| _version_ | 1819210961363402752 |
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| author | Laura-Isobel McCall Anupriya Tripathi Fernando Vargas Rob Knight Pieter C Dorrestein Jair L Siqueira-Neto |
| author_facet | Laura-Isobel McCall Anupriya Tripathi Fernando Vargas Rob Knight Pieter C Dorrestein Jair L Siqueira-Neto |
| author_sort | Laura-Isobel McCall |
| collection | DOAJ |
| description | Trypanosoma cruzi parasites are the causative agents of Chagas disease. These parasites infect cardiac and gastrointestinal tissues, leading to local inflammation and tissue damage. Digestive Chagas disease is associated with perturbations in food absorption, intestinal traffic and defecation. However, the impact of T. cruzi infection on the gut microbiota and metabolome have yet to be characterized. In this study, we applied mass spectrometry-based metabolomics and 16S rRNA sequencing to profile infection-associated alterations in fecal bacterial composition and fecal metabolome through the acute-stage and into the chronic stage of infection, in a murine model of Chagas disease. We observed joint microbial and chemical perturbations associated with T. cruzi infection. These included alterations in conjugated linoleic acid (CLA) derivatives and in specific members of families Ruminococcaceae and Lachnospiraceae, as well as alterations in secondary bile acids and members of order Clostridiales. These results highlight the importance of multi-'omics' and poly-microbial studies in understanding parasitic diseases in general, and Chagas disease in particular. |
| first_indexed | 2024-12-23T06:19:29Z |
| format | Article |
| id | doaj.art-ac6b8f77c35f40258945a77a5e6b06c1 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-23T06:19:29Z |
| publishDate | 2018-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-ac6b8f77c35f40258945a77a5e6b06c12022-12-21T17:57:14ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352018-03-01123e000634410.1371/journal.pntd.0006344Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome.Laura-Isobel McCallAnupriya TripathiFernando VargasRob KnightPieter C DorresteinJair L Siqueira-NetoTrypanosoma cruzi parasites are the causative agents of Chagas disease. These parasites infect cardiac and gastrointestinal tissues, leading to local inflammation and tissue damage. Digestive Chagas disease is associated with perturbations in food absorption, intestinal traffic and defecation. However, the impact of T. cruzi infection on the gut microbiota and metabolome have yet to be characterized. In this study, we applied mass spectrometry-based metabolomics and 16S rRNA sequencing to profile infection-associated alterations in fecal bacterial composition and fecal metabolome through the acute-stage and into the chronic stage of infection, in a murine model of Chagas disease. We observed joint microbial and chemical perturbations associated with T. cruzi infection. These included alterations in conjugated linoleic acid (CLA) derivatives and in specific members of families Ruminococcaceae and Lachnospiraceae, as well as alterations in secondary bile acids and members of order Clostridiales. These results highlight the importance of multi-'omics' and poly-microbial studies in understanding parasitic diseases in general, and Chagas disease in particular.http://europepmc.org/articles/PMC5864088?pdf=render |
| spellingShingle | Laura-Isobel McCall Anupriya Tripathi Fernando Vargas Rob Knight Pieter C Dorrestein Jair L Siqueira-Neto Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome. PLoS Neglected Tropical Diseases |
| title | Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome. |
| title_full | Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome. |
| title_fullStr | Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome. |
| title_full_unstemmed | Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome. |
| title_short | Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome. |
| title_sort | experimental chagas disease induced perturbations of the fecal microbiome and metabolome |
| url | http://europepmc.org/articles/PMC5864088?pdf=render |
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