High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in China
The aim of this work was to investigate the epidemiological and genetic characteristics of ESBL-producing Klebsiella pneumoniae (ESBL-Kp) causing community-onset infections. K. pneumoniae isolates were collected from 31 Chinese secondary hospitals between August 2010 and August 2011. Genes encoding...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01830/full |
| _version_ | 1818062603089346560 |
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| author | Jing Zhang Jing Zhang Kai Zhou Beiwen Zheng Lina Zhao Ping Shen Jinru Ji Zeqing Wei Lanjuan Li Jianying Zhou Yonghong Xiao |
| author_facet | Jing Zhang Jing Zhang Kai Zhou Beiwen Zheng Lina Zhao Ping Shen Jinru Ji Zeqing Wei Lanjuan Li Jianying Zhou Yonghong Xiao |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | The aim of this work was to investigate the epidemiological and genetic characteristics of ESBL-producing Klebsiella pneumoniae (ESBL-Kp) causing community-onset infections. K. pneumoniae isolates were collected from 31 Chinese secondary hospitals between August 2010 and August 2011. Genes encoding ESBL and AmpC beta-lactamases were detected by PCR. The isolates were assigned to sequence types (ST) using multi-locus sequence typing (MLST) and plasmid content was determined by PCR-based replicon typing. Eleven ESBL-Kp strains representing were selected to whole-genome sequencing (WGS) for investigating the genetic environment and plasmids encoding ESBL genes. A total of 578 K. pneumoniae isolates were collected, and 184 (31.8%) carried ESBL genes. The prevalence of ESBL-Kp varied from different geographical areas of China (10.2%-50.3%). The three most prevalent ESBL genes were blaCTX-M-14 (n=74), blaCTX-M-15 (n=60), and blaCTX-M-3 (n=40). MLST assigned 127 CTX-M-14 and CTX-M-15 producers to 54 STs, and CC17 was the most prevalent population (12.6%). STs (23, 37 and 86) that were known frequently associating with hypervirulent K. pneumoniae (hvKP) account for 14.1% (18/127). Phylogenetic analysis by concatenating the 7 loci of MLST revealed the existence of ESBL-producing K. quasipneumoniae (2 strains) and K. varricola (1 strain), which was further confirmed by WGS. IncFII type (n=130, 70.7%) was the predominant plasmid replicon. WGS analysis revealed various transmission mechanisms employed by different CTX-M genes. This study highlights the challenge of community-onset infections caused by ESBL-Kp in China. The prevalence of STs frequently associating with hvKP should be of concern. Surveillance of ESBL-KP causing community-onset infections now appears imperative. |
| first_indexed | 2024-12-10T14:06:50Z |
| format | Article |
| id | doaj.art-ac6d3ab94d95429196860601b99a9b8b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T14:06:50Z |
| publishDate | 2016-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-ac6d3ab94d95429196860601b99a9b8b2022-12-22T01:45:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01830231776High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in ChinaJing Zhang0Jing Zhang1Kai Zhou2Beiwen Zheng3Lina Zhao4Ping Shen5Jinru Ji6Zeqing Wei7Lanjuan Li8Jianying Zhou9Yonghong Xiao10Zhejiang UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversitySoochow UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversityThe aim of this work was to investigate the epidemiological and genetic characteristics of ESBL-producing Klebsiella pneumoniae (ESBL-Kp) causing community-onset infections. K. pneumoniae isolates were collected from 31 Chinese secondary hospitals between August 2010 and August 2011. Genes encoding ESBL and AmpC beta-lactamases were detected by PCR. The isolates were assigned to sequence types (ST) using multi-locus sequence typing (MLST) and plasmid content was determined by PCR-based replicon typing. Eleven ESBL-Kp strains representing were selected to whole-genome sequencing (WGS) for investigating the genetic environment and plasmids encoding ESBL genes. A total of 578 K. pneumoniae isolates were collected, and 184 (31.8%) carried ESBL genes. The prevalence of ESBL-Kp varied from different geographical areas of China (10.2%-50.3%). The three most prevalent ESBL genes were blaCTX-M-14 (n=74), blaCTX-M-15 (n=60), and blaCTX-M-3 (n=40). MLST assigned 127 CTX-M-14 and CTX-M-15 producers to 54 STs, and CC17 was the most prevalent population (12.6%). STs (23, 37 and 86) that were known frequently associating with hypervirulent K. pneumoniae (hvKP) account for 14.1% (18/127). Phylogenetic analysis by concatenating the 7 loci of MLST revealed the existence of ESBL-producing K. quasipneumoniae (2 strains) and K. varricola (1 strain), which was further confirmed by WGS. IncFII type (n=130, 70.7%) was the predominant plasmid replicon. WGS analysis revealed various transmission mechanisms employed by different CTX-M genes. This study highlights the challenge of community-onset infections caused by ESBL-Kp in China. The prevalence of STs frequently associating with hvKP should be of concern. Surveillance of ESBL-KP causing community-onset infections now appears imperative.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01830/fullresistance mechanismsCTX-Msequence typeCommunity-onset infectionshypervirulent K. pneumonia |
| spellingShingle | Jing Zhang Jing Zhang Kai Zhou Beiwen Zheng Lina Zhao Ping Shen Jinru Ji Zeqing Wei Lanjuan Li Jianying Zhou Yonghong Xiao High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in China Frontiers in Microbiology resistance mechanisms CTX-M sequence type Community-onset infections hypervirulent K. pneumonia |
| title | High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in China |
| title_full | High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in China |
| title_fullStr | High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in China |
| title_full_unstemmed | High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in China |
| title_short | High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in China |
| title_sort | high prevalence of esbl producing klebsiella pneumoniae causing community onset infections in china |
| topic | resistance mechanisms CTX-M sequence type Community-onset infections hypervirulent K. pneumonia |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01830/full |
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