Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo Studies
Curcumin is known to exhibit antioxidant and tissue-healing properties and has recently attracted the attention of the biomedical community for potential use in advanced therapies. This work reports the formulation and characterization of oil-in-water F127 microemulsions to enhance the bioavailabili...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/3/817 |
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| author | Abbas Rahdar Mohammad Reza Hajinezhad Saman Sargazi Maryam Zaboli Mahmood Barani Francesco Baino Muhammad Bilal Esmael Sanchooli |
| author_facet | Abbas Rahdar Mohammad Reza Hajinezhad Saman Sargazi Maryam Zaboli Mahmood Barani Francesco Baino Muhammad Bilal Esmael Sanchooli |
| author_sort | Abbas Rahdar |
| collection | DOAJ |
| description | Curcumin is known to exhibit antioxidant and tissue-healing properties and has recently attracted the attention of the biomedical community for potential use in advanced therapies. This work reports the formulation and characterization of oil-in-water F127 microemulsions to enhance the bioavailability of curcumin Microemulsions showed a high encapsulation efficiency and prolonged release. To investigate the interactions of curcumin with one unit of the polymeric chain of surfactant F127, ethyl butyrate, and sodium octanoate, as well as the interaction between ethyl butyrate and one unit of the F127 polymer chain, the Density Functional Theory (DFT) calculations at the M06-2X level of theory, were performed in water solution. The MTT assay was used to assess the cytotoxicity of free and encapsulated curcumin on non-malignant and malignant cell lines. Combination effects were calculated according to Chou-Talalay’s principles. Results of in vitro studies indicated that MCF7 and HepG2 cells were more sensitive to curcumin microemulsions. Moreover, a synergistic relationship was observed between curcumin microemulsions and cisplatin in all affected fractions of MCF7 and HepG2 cells (CI < 0.9). For in vivo investigation, thioacetamide-intoxicated rats received thioacetamide (100 mg/kg Sc) followed by curcumin microemulsions (30 mg/kg Ip). Thioacetamide-intoxicated rats showed elevated serum liver enzymes, blood urea nitrogen (BUN), and creatinine levels, and a significant reduction in liver superoxide dismutase (SOD) and catalase (CAT) activities (<i>p</i> < 0.05). Curcumin microemulsions reduced liver enzymes and serum creatinine and increased the activity of antioxidant enzymes in thioacetamide-treated rats in comparison to the untreated thioacetamide-intoxicated group. Histopathological investigations confirmed the biochemical findings. Overall, the current results showed the desirable hepatoprotective, nephroprotective, and anti-cancer effects of curcumin microemulsions. |
| first_indexed | 2024-03-10T12:59:13Z |
| format | Article |
| id | doaj.art-ac6d78e05181487b898480be8ad32c81 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T12:59:13Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-ac6d78e05181487b898480be8ad32c812023-11-21T11:38:04ZengMDPI AGNanomaterials2079-49912021-03-0111381710.3390/nano11030817Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo StudiesAbbas Rahdar0Mohammad Reza Hajinezhad1Saman Sargazi2Maryam Zaboli3Mahmood Barani4Francesco Baino5Muhammad Bilal6Esmael Sanchooli7Department of Physics, University of Zabol, P.O. Box. 98613-35856, Zabol, IranBasic Veterinary Science Department, Veterinary Faculty, University of Zabol, P.O. Box. 98613-35856, Zabol, IranCellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan 98167-43463, IranDepartment of Chemistry, University of Birjand, Birjand 97174-34765, IranDepartment of Chemistry, Shahid Bahonar University of Kerman, Kerman 76169-14111, IranInstitute of Materials Physics and Engineering, Applied Science and Technology Department, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, ItalySchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaDepartment of Chemistry, University of Zabol, P.O. Box. 98613-35856, Zabol, PIranCurcumin is known to exhibit antioxidant and tissue-healing properties and has recently attracted the attention of the biomedical community for potential use in advanced therapies. This work reports the formulation and characterization of oil-in-water F127 microemulsions to enhance the bioavailability of curcumin Microemulsions showed a high encapsulation efficiency and prolonged release. To investigate the interactions of curcumin with one unit of the polymeric chain of surfactant F127, ethyl butyrate, and sodium octanoate, as well as the interaction between ethyl butyrate and one unit of the F127 polymer chain, the Density Functional Theory (DFT) calculations at the M06-2X level of theory, were performed in water solution. The MTT assay was used to assess the cytotoxicity of free and encapsulated curcumin on non-malignant and malignant cell lines. Combination effects were calculated according to Chou-Talalay’s principles. Results of in vitro studies indicated that MCF7 and HepG2 cells were more sensitive to curcumin microemulsions. Moreover, a synergistic relationship was observed between curcumin microemulsions and cisplatin in all affected fractions of MCF7 and HepG2 cells (CI < 0.9). For in vivo investigation, thioacetamide-intoxicated rats received thioacetamide (100 mg/kg Sc) followed by curcumin microemulsions (30 mg/kg Ip). Thioacetamide-intoxicated rats showed elevated serum liver enzymes, blood urea nitrogen (BUN), and creatinine levels, and a significant reduction in liver superoxide dismutase (SOD) and catalase (CAT) activities (<i>p</i> < 0.05). Curcumin microemulsions reduced liver enzymes and serum creatinine and increased the activity of antioxidant enzymes in thioacetamide-treated rats in comparison to the untreated thioacetamide-intoxicated group. Histopathological investigations confirmed the biochemical findings. Overall, the current results showed the desirable hepatoprotective, nephroprotective, and anti-cancer effects of curcumin microemulsions.https://www.mdpi.com/2079-4991/11/3/817biomaterialsnanocarrieroil-in-water F127 microemulsionscurcuminhepatoprotectiveantioxidant |
| spellingShingle | Abbas Rahdar Mohammad Reza Hajinezhad Saman Sargazi Maryam Zaboli Mahmood Barani Francesco Baino Muhammad Bilal Esmael Sanchooli Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo Studies Nanomaterials biomaterials nanocarrier oil-in-water F127 microemulsions curcumin hepatoprotective antioxidant |
| title | Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo Studies |
| title_full | Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo Studies |
| title_fullStr | Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo Studies |
| title_full_unstemmed | Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo Studies |
| title_short | Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo Studies |
| title_sort | biochemical ameliorative and cytotoxic effects of newly synthesized curcumin microemulsions evidence from in vitro and in vivo studies |
| topic | biomaterials nanocarrier oil-in-water F127 microemulsions curcumin hepatoprotective antioxidant |
| url | https://www.mdpi.com/2079-4991/11/3/817 |
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