Quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors.
Methylation profiling of tumor suppressor gene (TSGs) promoters is quickly becoming a powerful diagnostic tool for the early detection, prognosis, and even prediction of clinical response to treatment. Few studies address this in salivary gland tumors (SGTs); hence the promoter methylation profile o...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-05-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2877085?pdf=render |
| _version_ | 1818555494330007552 |
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| author | Megan L Durr Wojciech K Mydlarz Chunbo Shao Marianna L Zahurak Alice Y Chuang Mohammad O Hoque William H Westra Nanette J Liegeois Joseph A Califano David Sidransky Patrick K Ha |
| author_facet | Megan L Durr Wojciech K Mydlarz Chunbo Shao Marianna L Zahurak Alice Y Chuang Mohammad O Hoque William H Westra Nanette J Liegeois Joseph A Califano David Sidransky Patrick K Ha |
| author_sort | Megan L Durr |
| collection | DOAJ |
| description | Methylation profiling of tumor suppressor gene (TSGs) promoters is quickly becoming a powerful diagnostic tool for the early detection, prognosis, and even prediction of clinical response to treatment. Few studies address this in salivary gland tumors (SGTs); hence the promoter methylation profile of various TSGs was quantitatively assessed in primary SGT tissue to determine if tumor-specific alterations could be detected.DNA isolated from 78 tumor and 17 normal parotid gland specimens was assayed for promoter methylation status of 19 TSGs by fluorescence-based, quantitative methylation-specific PCR (qMSP). The data were utilized in a binary fashion as well as quantitatively (using a methylation quotient) allowing for better profiling and interpretation of results.The average number of methylation events across the studied genes was highest in salivary duct carcinoma (SDC), with a methylation value of 9.6, compared to the normal 4.5 (p<0.0003). There was a variable frequency and individual methylation quotient detected, depending on the TSG and the tumor type. When comparing normal, benign, and malignant SGTs, there was a statistically significant trend for increasing methylation in APC, Mint 1, PGP9.5, RAR-beta, and Timp3.Screening promoter methylation profiles in SGTs showed considerable heterogeneity. The methylation status of certain markers was surprisingly high in even normal salivary tissue, confirming the need for such controls. Several TSGs were found to be associated with malignant SGTs, especially SDC. Further study is needed to evaluate the potential use of these associations in the detection, prognosis, and therapeutic outcome of these rare tumors. |
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| id | doaj.art-ac6e9c54c38641f4aa5ff3b9f06610a6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T09:54:11Z |
| publishDate | 2010-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ac6e9c54c38641f4aa5ff3b9f06610a62022-12-22T00:28:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-05-0155e1082810.1371/journal.pone.0010828Quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors.Megan L DurrWojciech K MydlarzChunbo ShaoMarianna L ZahurakAlice Y ChuangMohammad O HoqueWilliam H WestraNanette J LiegeoisJoseph A CalifanoDavid SidranskyPatrick K HaMethylation profiling of tumor suppressor gene (TSGs) promoters is quickly becoming a powerful diagnostic tool for the early detection, prognosis, and even prediction of clinical response to treatment. Few studies address this in salivary gland tumors (SGTs); hence the promoter methylation profile of various TSGs was quantitatively assessed in primary SGT tissue to determine if tumor-specific alterations could be detected.DNA isolated from 78 tumor and 17 normal parotid gland specimens was assayed for promoter methylation status of 19 TSGs by fluorescence-based, quantitative methylation-specific PCR (qMSP). The data were utilized in a binary fashion as well as quantitatively (using a methylation quotient) allowing for better profiling and interpretation of results.The average number of methylation events across the studied genes was highest in salivary duct carcinoma (SDC), with a methylation value of 9.6, compared to the normal 4.5 (p<0.0003). There was a variable frequency and individual methylation quotient detected, depending on the TSG and the tumor type. When comparing normal, benign, and malignant SGTs, there was a statistically significant trend for increasing methylation in APC, Mint 1, PGP9.5, RAR-beta, and Timp3.Screening promoter methylation profiles in SGTs showed considerable heterogeneity. The methylation status of certain markers was surprisingly high in even normal salivary tissue, confirming the need for such controls. Several TSGs were found to be associated with malignant SGTs, especially SDC. Further study is needed to evaluate the potential use of these associations in the detection, prognosis, and therapeutic outcome of these rare tumors.http://europepmc.org/articles/PMC2877085?pdf=render |
| spellingShingle | Megan L Durr Wojciech K Mydlarz Chunbo Shao Marianna L Zahurak Alice Y Chuang Mohammad O Hoque William H Westra Nanette J Liegeois Joseph A Califano David Sidransky Patrick K Ha Quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors. PLoS ONE |
| title | Quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors. |
| title_full | Quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors. |
| title_fullStr | Quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors. |
| title_full_unstemmed | Quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors. |
| title_short | Quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors. |
| title_sort | quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors |
| url | http://europepmc.org/articles/PMC2877085?pdf=render |
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