Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus
ABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Santa Maria
2016-07-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217&lng=en&tlng=en |
| _version_ | 1818847769923682304 |
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| author | Rodolfo de Moraes Peixoto Renata de Moraes Peixoto Araújo Luciana Jatobá e Silva Peixoto Ariel Marques Reges Ana Paula Pereira Alves José Wilton Pinheiro Júnior Rinaldo Aparecido Mota Sérgio Santos Azevedo Mateus Matiuzzi da Costa |
| author_facet | Rodolfo de Moraes Peixoto Renata de Moraes Peixoto Araújo Luciana Jatobá e Silva Peixoto Ariel Marques Reges Ana Paula Pereira Alves José Wilton Pinheiro Júnior Rinaldo Aparecido Mota Sérgio Santos Azevedo Mateus Matiuzzi da Costa |
| author_sort | Rodolfo de Moraes Peixoto |
| collection | DOAJ |
| description | ABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious dose of 1.2x108CFU mL-1 and a volume of 1mL per mammary gland. The CMT and the SCC were used to detect subclinical mastitis. Bacterial culture (BC) was performed immediately after milk collection and was used as the gold standard. Four experimental time points were established (0, 24, 48 and 72 hours post-inoculation). Analysis of the ROC curve confirmed that the best combination of sensitivity and specificity were obtained with a cutoff point of 405.5, 6030.0 and 729.5x103 cells mL-1, respectively at M1, M2 and M3. Furthermore, considering the drop in sensitivity throughout the experimental time points, the use of serial bacterial cultures are recommended, particularly in herds with a high prevalence of S. aureus. |
| first_indexed | 2024-12-19T06:06:43Z |
| format | Article |
| id | doaj.art-ac6f9caf301442b58967de431459fb56 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-12-19T06:06:43Z |
| publishDate | 2016-07-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-ac6f9caf301442b58967de431459fb562022-12-21T20:33:06ZengUniversidade Federal de Santa MariaCiência Rural1678-45962016-07-014671217122210.1590/0103-8478cr20150507S0103-84782016000701217Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureusRodolfo de Moraes PeixotoRenata de Moraes Peixoto AraújoLuciana Jatobá e Silva PeixotoAriel Marques RegesAna Paula Pereira AlvesJosé Wilton Pinheiro JúniorRinaldo Aparecido MotaSérgio Santos AzevedoMateus Matiuzzi da CostaABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious dose of 1.2x108CFU mL-1 and a volume of 1mL per mammary gland. The CMT and the SCC were used to detect subclinical mastitis. Bacterial culture (BC) was performed immediately after milk collection and was used as the gold standard. Four experimental time points were established (0, 24, 48 and 72 hours post-inoculation). Analysis of the ROC curve confirmed that the best combination of sensitivity and specificity were obtained with a cutoff point of 405.5, 6030.0 and 729.5x103 cells mL-1, respectively at M1, M2 and M3. Furthermore, considering the drop in sensitivity throughout the experimental time points, the use of serial bacterial cultures are recommended, particularly in herds with a high prevalence of S. aureus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217&lng=en&tlng=encultura bacteriológicainfecção intramamáriapequenos ruminantescontagem de célula somática |
| spellingShingle | Rodolfo de Moraes Peixoto Renata de Moraes Peixoto Araújo Luciana Jatobá e Silva Peixoto Ariel Marques Reges Ana Paula Pereira Alves José Wilton Pinheiro Júnior Rinaldo Aparecido Mota Sérgio Santos Azevedo Mateus Matiuzzi da Costa Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus Ciência Rural cultura bacteriológica infecção intramamária pequenos ruminantes contagem de célula somática |
| title | Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
| title_full | Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
| title_fullStr | Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
| title_full_unstemmed | Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
| title_short | Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
| title_sort | indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with staphylococcus aureus |
| topic | cultura bacteriológica infecção intramamária pequenos ruminantes contagem de célula somática |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217&lng=en&tlng=en |
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