EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L.
This study was investigate the effect of components media and silver nanoparticles (Ag-NPs) synthesized in the laboratory on the induction of biomass and secondary metabolites of calli cultures induced from the cotyledons of Trigonella foenum-graecum L. The culture media LS and B5 were tested with...
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| Format: | Article |
| Language: | English |
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Baghdad University
2024-01-01
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| Series: | The Iraqi Journal of Agricultural science |
| Subjects: | |
| Online Access: | https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1891 |
| _version_ | 1797346379824103424 |
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| author | Masara D. J. S. I. Neamah |
| author_facet | Masara D. J. S. I. Neamah |
| author_sort | Masara D. J. |
| collection | DOAJ |
| description |
This study was investigate the effect of components media and silver nanoparticles (Ag-NPs) synthesized in the laboratory on the induction of biomass and secondary metabolites of calli cultures induced from the cotyledons of Trigonella foenum-graecum L. The culture media LS and B5 were tested with the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) at a concentration of 1.0, 2.0 and 3.0 mg l-1 and 6-benzyladenine (BA) at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1, with the aim of studying its effect on callus induction. In comparison, Ag-NPs were used at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1 to study its effect on the production of some secondary compounds. The results showed that the mixture consisting of the culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BA the highest percentage of callus induction and the minimum days to complete callus formation. While, the same media with 2.0 mg l-1 2,4-D and 1.0 mg l-1 of BA recorded the highest dry weight (DW) and relative water content (RWC). The combination B5×2.0 mg l-1 2,4-D×1.5 or 2.0 mg l-1 showed the highest fresh weight (FW) and the lowest time required to start callus induction, respectively. As for the production of SC, the B5 media with 2.0 mg l-1 of Ag-NPs achieved the highest concentration of alkaloid compounds represented by trigonelline and diosgenin, and flavonoids expressed by qurcetine, vitxein, rutin, apigenin, isovitexin, and kaemferol.
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| first_indexed | 2024-03-08T11:31:17Z |
| format | Article |
| id | doaj.art-ac75afe665a741c08aab29329adb014e |
| institution | Directory Open Access Journal |
| issn | 0075-0530 2410-0862 |
| language | English |
| last_indexed | 2024-03-08T11:31:17Z |
| publishDate | 2024-01-01 |
| publisher | Baghdad University |
| record_format | Article |
| series | The Iraqi Journal of Agricultural science |
| spelling | doaj.art-ac75afe665a741c08aab29329adb014e2024-01-25T18:24:41ZengBaghdad UniversityThe Iraqi Journal of Agricultural science0075-05302410-08622024-01-0155Special IssueEFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L.Masara D. J.S. I. Neamah This study was investigate the effect of components media and silver nanoparticles (Ag-NPs) synthesized in the laboratory on the induction of biomass and secondary metabolites of calli cultures induced from the cotyledons of Trigonella foenum-graecum L. The culture media LS and B5 were tested with the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) at a concentration of 1.0, 2.0 and 3.0 mg l-1 and 6-benzyladenine (BA) at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1, with the aim of studying its effect on callus induction. In comparison, Ag-NPs were used at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1 to study its effect on the production of some secondary compounds. The results showed that the mixture consisting of the culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BA the highest percentage of callus induction and the minimum days to complete callus formation. While, the same media with 2.0 mg l-1 2,4-D and 1.0 mg l-1 of BA recorded the highest dry weight (DW) and relative water content (RWC). The combination B5×2.0 mg l-1 2,4-D×1.5 or 2.0 mg l-1 showed the highest fresh weight (FW) and the lowest time required to start callus induction, respectively. As for the production of SC, the B5 media with 2.0 mg l-1 of Ag-NPs achieved the highest concentration of alkaloid compounds represented by trigonelline and diosgenin, and flavonoids expressed by qurcetine, vitxein, rutin, apigenin, isovitexin, and kaemferol. https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1891in vitro, nanoparticles, flavonoids, alkaloids. |
| spellingShingle | Masara D. J. S. I. Neamah EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L. The Iraqi Journal of Agricultural science in vitro, nanoparticles, flavonoids, alkaloids. |
| title | EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L. |
| title_full | EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L. |
| title_fullStr | EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L. |
| title_full_unstemmed | EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L. |
| title_short | EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L. |
| title_sort | effect of chemical media components and silver nanoparticles on the induction of callus culture and accumulation of secondary compounds in a trigonella foenum graecum l |
| topic | in vitro, nanoparticles, flavonoids, alkaloids. |
| url | https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1891 |
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