Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling.
BACKGROUND: Mating decreases female receptivity and terminates sex pheromone production in moths. Although significant progress has been made in elucidating the mating-regulated inactivation of pheromone biosynthesis-activating neuropeptide (PBAN) secretion, little is known about the mating induced...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4203833?pdf=render |
| _version_ | 1818618300177842176 |
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| author | Songdou Zhang Xiaoming Liu Bin Zhu Xinming Yin Mengfang Du Qisheng Song Shiheng An |
| author_facet | Songdou Zhang Xiaoming Liu Bin Zhu Xinming Yin Mengfang Du Qisheng Song Shiheng An |
| author_sort | Songdou Zhang |
| collection | DOAJ |
| description | BACKGROUND: Mating decreases female receptivity and terminates sex pheromone production in moths. Although significant progress has been made in elucidating the mating-regulated inactivation of pheromone biosynthesis-activating neuropeptide (PBAN) secretion, little is known about the mating induced gene expression profiles in pheromone glands (PGs). In this study, the associated genes involved in Bombyx mori mating were identified through digital gene expression (DGE) profiling and subsequent RNA interference (RNAi) to elucidate the molecular mechanisms underlying the mating-regulated gene expression in PGs. RESULTS: Eight DGE libraries were constructed from the PGs of mated and virgin females: 1 h mating (M1)/virgin (V1) PGs, 3 h mating (M3)/virgin (V3) PGs, 24 h mating (M24)/virgin (V24) PGs and 48 h mating (M48)/virgin (V48) PGs (M48 and V48). These libraries were used to investigate the gene expression profiles affected by mating. DGE profiling revealed a series of genes showing differential expression in each set of mated and virgin female samples, including immune-associated genes, sex pheromone synthesis-associated genes, juvenile hormone (JH) signal-associated genes, etc. Most interestingly, JH signal was found to be activated by mating. Application of the JH mimics, methoprene to the newly-emerged virgin females leaded to the significant reduction of sex pheromone production. RNAi-mediated knockdown of putative JH receptor gene, Methoprene tolerant 1 (Met1), in female pupa resulted in a significant decrease in sex pheromone production in mature females, suggesting the importance of JH in sex pheromone synthesis. CONCLUSION: A series of differentially expressed genes in PGs in response to mating was identified. This study improves our understanding of the role of JH signaling on the mating-elicited termination of sex pheromone production. |
| first_indexed | 2024-12-16T17:19:24Z |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T17:19:24Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ac76a64694794ee68b2755711414eed72022-12-21T22:23:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11100310.1371/journal.pone.0111003Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling.Songdou ZhangXiaoming LiuBin ZhuXinming YinMengfang DuQisheng SongShiheng AnBACKGROUND: Mating decreases female receptivity and terminates sex pheromone production in moths. Although significant progress has been made in elucidating the mating-regulated inactivation of pheromone biosynthesis-activating neuropeptide (PBAN) secretion, little is known about the mating induced gene expression profiles in pheromone glands (PGs). In this study, the associated genes involved in Bombyx mori mating were identified through digital gene expression (DGE) profiling and subsequent RNA interference (RNAi) to elucidate the molecular mechanisms underlying the mating-regulated gene expression in PGs. RESULTS: Eight DGE libraries were constructed from the PGs of mated and virgin females: 1 h mating (M1)/virgin (V1) PGs, 3 h mating (M3)/virgin (V3) PGs, 24 h mating (M24)/virgin (V24) PGs and 48 h mating (M48)/virgin (V48) PGs (M48 and V48). These libraries were used to investigate the gene expression profiles affected by mating. DGE profiling revealed a series of genes showing differential expression in each set of mated and virgin female samples, including immune-associated genes, sex pheromone synthesis-associated genes, juvenile hormone (JH) signal-associated genes, etc. Most interestingly, JH signal was found to be activated by mating. Application of the JH mimics, methoprene to the newly-emerged virgin females leaded to the significant reduction of sex pheromone production. RNAi-mediated knockdown of putative JH receptor gene, Methoprene tolerant 1 (Met1), in female pupa resulted in a significant decrease in sex pheromone production in mature females, suggesting the importance of JH in sex pheromone synthesis. CONCLUSION: A series of differentially expressed genes in PGs in response to mating was identified. This study improves our understanding of the role of JH signaling on the mating-elicited termination of sex pheromone production.http://europepmc.org/articles/PMC4203833?pdf=render |
| spellingShingle | Songdou Zhang Xiaoming Liu Bin Zhu Xinming Yin Mengfang Du Qisheng Song Shiheng An Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling. PLoS ONE |
| title | Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling. |
| title_full | Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling. |
| title_fullStr | Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling. |
| title_full_unstemmed | Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling. |
| title_short | Identification of differentially expressed genes in the pheromone glands of mated and virgin Bombyx mori by digital gene expression profiling. |
| title_sort | identification of differentially expressed genes in the pheromone glands of mated and virgin bombyx mori by digital gene expression profiling |
| url | http://europepmc.org/articles/PMC4203833?pdf=render |
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