The effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solvents
This study aimed to determine the effect of two mycorrhizal fungi, Funneliformis mosseae (Fm) and Rhizophagus intraradices (Ri), on capsaicinoid production in Capsicum annuum L. by gas chromatography-mass spectrometry (GC-MS) via two different extraction approaches, magnetic stirring and ultrasound-...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2023-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300027C.pdf |
| _version_ | 1797393751775117312 |
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| author | Canpolat Şükrü İşlek Cemil |
| author_facet | Canpolat Şükrü İşlek Cemil |
| author_sort | Canpolat Şükrü |
| collection | DOAJ |
| description | This study aimed to determine the effect of two mycorrhizal fungi, Funneliformis mosseae (Fm) and Rhizophagus intraradices (Ri), on capsaicinoid production in Capsicum annuum L. by gas chromatography-mass spectrometry (GC-MS) via two different extraction approaches, magnetic stirring and ultrasound-assisted extraction with three different solvents, ethanol (EtOH), ethyl acetate (EtAce), and acetonitrile (AceN). The effect of mycorrhizal fungi on some physiological properties and biochemical activity, the content of total phenolic compounds, and antioxidant activity were also investigated. For all investigated parameters, the plants inoculated with mycorrhizal fungi showed significantly higher values than the non-mycorrhizal control plants, except for malondialdehyde (MDA), which was an indicator of lipid peroxidation due to damage that occurred in the cell membrane. It was concluded that inoculation with mycorrhizal fungi increased both capsaicin and dihydrocapsaicin production up to 4-fold in C. annuum compared to the control. Results also indicated that ultrasound-assisted extraction with EtAce was the most effective method for the determination of capsaicin by GC-MS. |
| first_indexed | 2024-03-09T00:07:25Z |
| format | Article |
| id | doaj.art-ac77a77b6dc24e528491a043ddbdc523 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-03-09T00:07:25Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-ac77a77b6dc24e528491a043ddbdc5232023-12-12T13:06:47ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392023-01-0175332733910.2298/ABS230601027C0354-46642300027CThe effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solventsCanpolat Şükrü0İşlek Cemil1Central Research Laboratory Center, Niğde Ömer Halisdemir University, Niğde, TürkiyeDepartment of Biotechnology, Faculty of Arts and Sciences, Niğde Ömer Halisdemir University, Niğde, TürkiyeThis study aimed to determine the effect of two mycorrhizal fungi, Funneliformis mosseae (Fm) and Rhizophagus intraradices (Ri), on capsaicinoid production in Capsicum annuum L. by gas chromatography-mass spectrometry (GC-MS) via two different extraction approaches, magnetic stirring and ultrasound-assisted extraction with three different solvents, ethanol (EtOH), ethyl acetate (EtAce), and acetonitrile (AceN). The effect of mycorrhizal fungi on some physiological properties and biochemical activity, the content of total phenolic compounds, and antioxidant activity were also investigated. For all investigated parameters, the plants inoculated with mycorrhizal fungi showed significantly higher values than the non-mycorrhizal control plants, except for malondialdehyde (MDA), which was an indicator of lipid peroxidation due to damage that occurred in the cell membrane. It was concluded that inoculation with mycorrhizal fungi increased both capsaicin and dihydrocapsaicin production up to 4-fold in C. annuum compared to the control. Results also indicated that ultrasound-assisted extraction with EtAce was the most effective method for the determination of capsaicin by GC-MS.https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300027C.pdfcapsicum annuumfunneliformis mosseaemycorrhizacapsaicinultrasound-assisted extractiongas chromatography-mass spectrometry (gc-ms) |
| spellingShingle | Canpolat Şükrü İşlek Cemil The effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solvents Archives of Biological Sciences capsicum annuum funneliformis mosseae mycorrhiza capsaicin ultrasound-assisted extraction gas chromatography-mass spectrometry (gc-ms) |
| title | The effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solvents |
| title_full | The effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solvents |
| title_fullStr | The effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solvents |
| title_full_unstemmed | The effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solvents |
| title_short | The effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solvents |
| title_sort | effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in capsicum annuum l a comparative study of extraction methods and solvents |
| topic | capsicum annuum funneliformis mosseae mycorrhiza capsaicin ultrasound-assisted extraction gas chromatography-mass spectrometry (gc-ms) |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300027C.pdf |
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