ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM
Activity of cellulolytic enzymes cultures Stereum hirsutum (Wild.) Gray, different nuclear status, depending on the substrate have been studied. The most productive by synthesis endoglucanases monokaryotic cultures of Sh1-4 and Sh7-2 were determined. It is found that nutrient medium containing filte...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2013-06-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/no3_2013/boikoryazan_3_2013.pdf |
| _version_ | 1797728777381347328 |
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| author | S. M. Boiko M. E. Ryazanova |
| author_facet | S. M. Boiko M. E. Ryazanova |
| author_sort | S. M. Boiko |
| collection | DOAJ |
| description | Activity of cellulolytic enzymes cultures Stereum hirsutum (Wild.) Gray, different nuclear status, depending on the substrate have been studied. The most productive by synthesis endoglucanases monokaryotic cultures of Sh1-4 and Sh7-2 were determined. It is found that nutrient medium containing filtering paper is optimal for synthesis of cellulase (culture Sh1-4, maximum of cellulolytic activity is 319 ?М/mg, culture Sh7-2 — 101 ?М/mg). Maximum of cellulolytic activity at cultivation with sawdust of trees for culture Sh1-4 observed at the 10th day was 142 ?М/mg (oak sawdust), for culture Sh7-2 at the 20th days it was 34 ?М/mg (ash-tree sawdust). Study of quality cellulolytic enzymes enabled to establishe presence of four exoenzymes of Cx complex with a relative electrophoretic mobility of 0.22; 0.24; 0.47; 0.74. Enzyme with relative electrophoretic mobility 0.47 showed high concentration and activity. |
| first_indexed | 2024-03-12T11:19:01Z |
| format | Article |
| id | doaj.art-ac77abdafde942b8a0a64f072ff923f8 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T11:19:01Z |
| publishDate | 2013-06-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-ac77abdafde942b8a0a64f072ff923f82023-09-02T01:27:51ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2013-06-016311612010.15407/biotech6.03.116ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUMS. M. Boiko0M. E. Ryazanova1Donetsk national university, UkraineDonetsk national university, UkraineActivity of cellulolytic enzymes cultures Stereum hirsutum (Wild.) Gray, different nuclear status, depending on the substrate have been studied. The most productive by synthesis endoglucanases monokaryotic cultures of Sh1-4 and Sh7-2 were determined. It is found that nutrient medium containing filtering paper is optimal for synthesis of cellulase (culture Sh1-4, maximum of cellulolytic activity is 319 ?М/mg, culture Sh7-2 — 101 ?М/mg). Maximum of cellulolytic activity at cultivation with sawdust of trees for culture Sh1-4 observed at the 10th day was 142 ?М/mg (oak sawdust), for culture Sh7-2 at the 20th days it was 34 ?М/mg (ash-tree sawdust). Study of quality cellulolytic enzymes enabled to establishe presence of four exoenzymes of Cx complex with a relative electrophoretic mobility of 0.22; 0.24; 0.47; 0.74. Enzyme with relative electrophoretic mobility 0.47 showed high concentration and activity.http://biotechnology.kiev.ua/images/storage/no3_2013/boikoryazan_3_2013.pdfcellulolytic enzymesStereum hirsutummonokaryotic cultureselectrophoresis of proteins. |
| spellingShingle | S. M. Boiko M. E. Ryazanova ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM Biotechnologia Acta cellulolytic enzymes Stereum hirsutum monokaryotic cultures electrophoresis of proteins. |
| title | ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM |
| title_full | ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM |
| title_fullStr | ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM |
| title_full_unstemmed | ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM |
| title_short | ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM |
| title_sort | activity dependence of cellulolytic enzymes monoand dikaryotic cultures of stereum hirsutum wild gray basidiomycetes from a substratum |
| topic | cellulolytic enzymes Stereum hirsutum monokaryotic cultures electrophoresis of proteins. |
| url | http://biotechnology.kiev.ua/images/storage/no3_2013/boikoryazan_3_2013.pdf |
| work_keys_str_mv | AT smboiko activitydependenceofcellulolyticenzymesmonoanddikaryoticculturesofstereumhirsutumwildgraybasidiomycetesfromasubstratum AT meryazanova activitydependenceofcellulolyticenzymesmonoanddikaryoticculturesofstereumhirsutumwildgraybasidiomycetesfromasubstratum |