Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive Cells
Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that causes huge losses economically to the pig industry worldwide. Previous research suggested that receptor dependence is necessary for PRRSV infection. MYH9 and CD163 are indispensable for PRRSV entry into a porc...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.865343/full |
| _version_ | 1817986844357296128 |
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| author | Liangliang Li Weiyao Sun Qifan Hu Tongtong Wang Guang Zhu Qin Zhao En-Min Zhou |
| author_facet | Liangliang Li Weiyao Sun Qifan Hu Tongtong Wang Guang Zhu Qin Zhao En-Min Zhou |
| author_sort | Liangliang Li |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that causes huge losses economically to the pig industry worldwide. Previous research suggested that receptor dependence is necessary for PRRSV infection. MYH9 and CD163 are indispensable for PRRSV entry into a porcine alveolar macrophage. In the present study, human MYH9 (hMYH9) and mouse MYH9 (mMYH9), similar to swine MYH9, could also accelerate PRRSV infection in pCD163-mediated cell lines. Knockdown of MYH9 activity using the specific small interfering RNA or inhibitor (blebbistatin) concomitantly decreased PRRSV infection. C-terminal fragment of MYH9 (PRA) proteins from different mammalian species contains a conserved binding domain (aa1676-1791) for PRRSV binding, since the recombinant MYH91676−1791protein could inhibit the PRRSV infection significantly. Furthermore, the specific polyclonal antibody of MYH91676−1791 could block PRRSV infection in host cells. These data strongly supported that MYH9, a very important cofactor, participated in PRRSV entry into target cells, which may facilitate the development of a new therapeutic agent to control PRRSV infection. |
| first_indexed | 2024-04-14T00:14:26Z |
| format | Article |
| id | doaj.art-ac77d5fc9e8c4589bb7a0e54b65a0b9e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T00:14:26Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ac77d5fc9e8c4589bb7a0e54b65a0b9e2022-12-22T02:23:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.865343865343Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive CellsLiangliang Li0Weiyao Sun1Qifan Hu2Tongtong Wang3Guang Zhu4Qin Zhao5En-Min Zhou6College of Agronomy, Liaocheng University, Liaocheng, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A and F University, Xianyang, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A and F University, Xianyang, ChinaCollege of Agronomy, Liaocheng University, Liaocheng, ChinaShandong Vocational Animal Science and Veterinary College, Weifang, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A and F University, Xianyang, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A and F University, Xianyang, ChinaPorcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that causes huge losses economically to the pig industry worldwide. Previous research suggested that receptor dependence is necessary for PRRSV infection. MYH9 and CD163 are indispensable for PRRSV entry into a porcine alveolar macrophage. In the present study, human MYH9 (hMYH9) and mouse MYH9 (mMYH9), similar to swine MYH9, could also accelerate PRRSV infection in pCD163-mediated cell lines. Knockdown of MYH9 activity using the specific small interfering RNA or inhibitor (blebbistatin) concomitantly decreased PRRSV infection. C-terminal fragment of MYH9 (PRA) proteins from different mammalian species contains a conserved binding domain (aa1676-1791) for PRRSV binding, since the recombinant MYH91676−1791protein could inhibit the PRRSV infection significantly. Furthermore, the specific polyclonal antibody of MYH91676−1791 could block PRRSV infection in host cells. These data strongly supported that MYH9, a very important cofactor, participated in PRRSV entry into target cells, which may facilitate the development of a new therapeutic agent to control PRRSV infection.https://www.frontiersin.org/articles/10.3389/fmicb.2022.865343/fullPRRSVMYH9virus entryaa1676-1791antiviral |
| spellingShingle | Liangliang Li Weiyao Sun Qifan Hu Tongtong Wang Guang Zhu Qin Zhao En-Min Zhou Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive Cells Frontiers in Microbiology PRRSV MYH9 virus entry aa1676-1791 antiviral |
| title | Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive Cells |
| title_full | Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive Cells |
| title_fullStr | Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive Cells |
| title_full_unstemmed | Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive Cells |
| title_short | Identification of MYH9 Key Domain Involved in the Entry of PRRSV Into Permissive Cells |
| title_sort | identification of myh9 key domain involved in the entry of prrsv into permissive cells |
| topic | PRRSV MYH9 virus entry aa1676-1791 antiviral |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.865343/full |
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