The Properties of Salam Leaf Extract (Syzygium Polyanthum) with Different Solvent Ratio and Processing Time Using Ultrasonication-Assisted Extraction Method

Salam leaf (Syzygium polyanthum) is an Indonesian traditional herbs with high bioactive compound potential. Unfortunately, as an alternative method that is friendly to heat-susceptible bioactive compound, the ultrasonication extraction method has not been widely used to extract Salam leaves. Meanwh...

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Main Authors: Bima Putra Pratama, Yudi Pranoto, Supriyadi, Respati Tri Swasono
Format: Article
Language:English
Published: Tamkang University Press 2022-09-01
Series:Journal of Applied Science and Engineering
Subjects:
Online Access:http://jase.tku.edu.tw/articles/jase-202304-26-4-0015
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author Bima Putra Pratama
Yudi Pranoto
Supriyadi
Respati Tri Swasono
author_facet Bima Putra Pratama
Yudi Pranoto
Supriyadi
Respati Tri Swasono
author_sort Bima Putra Pratama
collection DOAJ
description Salam leaf (Syzygium polyanthum) is an Indonesian traditional herbs with high bioactive compound potential. Unfortunately, as an alternative method that is friendly to heat-susceptible bioactive compound, the ultrasonication extraction method has not been widely used to extract Salam leaves. Meanwhile, the processing time and the ratio of the use of solvents are some of the determinants of the performance of this method. Hence, this research examined the ultrasonication method for extracting bioactive compounds from Salam leaf from the time and ratio of the solvent used. The results were showed that the best condition was from the solvent ratio to sample of 12:1 for 15 min extraction time. These conditions gave results in an 18.57% yield of salam leaf extract, 74.20 ± 0.19 mg Gallic acid equivalent/g salam leaf extract of total phenolic content, 22.00 ± 0.08 mg Quercetin equivalent/g salam leave extract of total flavonoid content, 78.79% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, and IC50 antioxidant activity of 20.25 µg/ml salam leave extract. The best extraction conditions are able to provide Salam leaf extract with very strong antioxidant potential.
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spelling doaj.art-ac781f91a9784f699e207e3585a6c36c2022-12-22T01:44:45ZengTamkang University PressJournal of Applied Science and Engineering2708-99672708-99752022-09-01264 58158710.6180/jase.202304_26(4).0015The Properties of Salam Leaf Extract (Syzygium Polyanthum) with Different Solvent Ratio and Processing Time Using Ultrasonication-Assisted Extraction MethodBima Putra Pratama0Yudi Pranoto1Supriyadi2Respati Tri Swasono3Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281, IndonesiaDepartment of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281, IndonesiaDepartment of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Jl. Sekip Utara, Yogyakarta 55281, IndonesiaSalam leaf (Syzygium polyanthum) is an Indonesian traditional herbs with high bioactive compound potential. Unfortunately, as an alternative method that is friendly to heat-susceptible bioactive compound, the ultrasonication extraction method has not been widely used to extract Salam leaves. Meanwhile, the processing time and the ratio of the use of solvents are some of the determinants of the performance of this method. Hence, this research examined the ultrasonication method for extracting bioactive compounds from Salam leaf from the time and ratio of the solvent used. The results were showed that the best condition was from the solvent ratio to sample of 12:1 for 15 min extraction time. These conditions gave results in an 18.57% yield of salam leaf extract, 74.20 ± 0.19 mg Gallic acid equivalent/g salam leaf extract of total phenolic content, 22.00 ± 0.08 mg Quercetin equivalent/g salam leave extract of total flavonoid content, 78.79% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, and IC50 antioxidant activity of 20.25 µg/ml salam leave extract. The best extraction conditions are able to provide Salam leaf extract with very strong antioxidant potential.http://jase.tku.edu.tw/articles/jase-202304-26-4-0015salam leafultrasonication methodbioactive compoundantioxidant activity
spellingShingle Bima Putra Pratama
Yudi Pranoto
Supriyadi
Respati Tri Swasono
The Properties of Salam Leaf Extract (Syzygium Polyanthum) with Different Solvent Ratio and Processing Time Using Ultrasonication-Assisted Extraction Method
Journal of Applied Science and Engineering
salam leaf
ultrasonication method
bioactive compound
antioxidant activity
title The Properties of Salam Leaf Extract (Syzygium Polyanthum) with Different Solvent Ratio and Processing Time Using Ultrasonication-Assisted Extraction Method
title_full The Properties of Salam Leaf Extract (Syzygium Polyanthum) with Different Solvent Ratio and Processing Time Using Ultrasonication-Assisted Extraction Method
title_fullStr The Properties of Salam Leaf Extract (Syzygium Polyanthum) with Different Solvent Ratio and Processing Time Using Ultrasonication-Assisted Extraction Method
title_full_unstemmed The Properties of Salam Leaf Extract (Syzygium Polyanthum) with Different Solvent Ratio and Processing Time Using Ultrasonication-Assisted Extraction Method
title_short The Properties of Salam Leaf Extract (Syzygium Polyanthum) with Different Solvent Ratio and Processing Time Using Ultrasonication-Assisted Extraction Method
title_sort properties of salam leaf extract syzygium polyanthum with different solvent ratio and processing time using ultrasonication assisted extraction method
topic salam leaf
ultrasonication method
bioactive compound
antioxidant activity
url http://jase.tku.edu.tw/articles/jase-202304-26-4-0015
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