Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selectedmicroscopic filamentous fungi
Vicine and convicine were isolated from mature seeds of Vicia faba L. These compounds were separated from the relevant fractions by HPLC and identified by IR, MS and NMR. Obtained vicine was used for all bioassays. Vicine in concentration of 25 µg in 1 ml of medium had significant toxic effect. The...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2002-12-01
|
| Series: | Plant, Soil and Environment |
| Subjects: | |
| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-200212-0004_fungitoxicity-of-natural-heterocycle-glucoside-vicine-obtained-from-vicia-faba-l-against-selectedmicroscopic-f.php |
| _version_ | 1797898432664305664 |
|---|---|
| author | M. Pavlík M. Váňová V. Laudová J. Harmatha |
| author_facet | M. Pavlík M. Váňová V. Laudová J. Harmatha |
| author_sort | M. Pavlík |
| collection | DOAJ |
| description | Vicine and convicine were isolated from mature seeds of Vicia faba L. These compounds were separated from the relevant fractions by HPLC and identified by IR, MS and NMR. Obtained vicine was used for all bioassays. Vicine in concentration of 25 µg in 1 ml of medium had significant toxic effect. The concentration of 5 µg of vicine in 1 ml of medium had significant fungistatic effect for fungi Fusarium culmorum and Alternaria alternata. The concentration of 500 ng of vicine in 1 ml of medium had significant fungistatic effect for fungi Cladosporium herbarum and Botrytis cinerea. The effective concentration of vicine in our test was significantly lower than is the concentration of vicine in seeds and pods of V. faba. The toxicity of vicine comparing with the toxicity of insecticide Actellic was higher by one order. Also, the fungicide Impact had a lower toxicity than vicine. Fungitoxic and fungistatic effect of vicine was comparable with some other tested fungicides and with the plant saponin digitonin. Our results confirmed that vicine is important natural fungicide. |
| first_indexed | 2024-04-10T08:14:23Z |
| format | Article |
| id | doaj.art-ac79995c05124f3e8820beb38c70788c |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:14:23Z |
| publishDate | 2002-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-ac79995c05124f3e8820beb38c70788c2023-02-23T03:44:46ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682002-12-01481254354710.17221/4410-PSEpse-200212-0004Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selectedmicroscopic filamentous fungiM. Pavlík0M. Váňová1V. Laudová2J. Harmatha3Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech RepublicVicine and convicine were isolated from mature seeds of Vicia faba L. These compounds were separated from the relevant fractions by HPLC and identified by IR, MS and NMR. Obtained vicine was used for all bioassays. Vicine in concentration of 25 µg in 1 ml of medium had significant toxic effect. The concentration of 5 µg of vicine in 1 ml of medium had significant fungistatic effect for fungi Fusarium culmorum and Alternaria alternata. The concentration of 500 ng of vicine in 1 ml of medium had significant fungistatic effect for fungi Cladosporium herbarum and Botrytis cinerea. The effective concentration of vicine in our test was significantly lower than is the concentration of vicine in seeds and pods of V. faba. The toxicity of vicine comparing with the toxicity of insecticide Actellic was higher by one order. Also, the fungicide Impact had a lower toxicity than vicine. Fungitoxic and fungistatic effect of vicine was comparable with some other tested fungicides and with the plant saponin digitonin. Our results confirmed that vicine is important natural fungicide.https://pse.agriculturejournals.cz/artkey/pse-200212-0004_fungitoxicity-of-natural-heterocycle-glucoside-vicine-obtained-from-vicia-faba-l-against-selectedmicroscopic-f.phpvicineconvicinepirimiphos-methylflutriafolpropiconazolecarbendazimfungicide bioassayfusarium culmorumcladosporium herbarumalternaria alternatabotrytis cinerea |
| spellingShingle | M. Pavlík M. Váňová V. Laudová J. Harmatha Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selectedmicroscopic filamentous fungi Plant, Soil and Environment vicine convicine pirimiphos-methyl flutriafol propiconazole carbendazim fungicide bioassay fusarium culmorum cladosporium herbarum alternaria alternata botrytis cinerea |
| title | Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selectedmicroscopic filamentous fungi |
| title_full | Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selectedmicroscopic filamentous fungi |
| title_fullStr | Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selectedmicroscopic filamentous fungi |
| title_full_unstemmed | Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selectedmicroscopic filamentous fungi |
| title_short | Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selectedmicroscopic filamentous fungi |
| title_sort | fungitoxicity of natural heterocycle glucoside vicine obtained from vicia faba l against selectedmicroscopic filamentous fungi |
| topic | vicine convicine pirimiphos-methyl flutriafol propiconazole carbendazim fungicide bioassay fusarium culmorum cladosporium herbarum alternaria alternata botrytis cinerea |
| url | https://pse.agriculturejournals.cz/artkey/pse-200212-0004_fungitoxicity-of-natural-heterocycle-glucoside-vicine-obtained-from-vicia-faba-l-against-selectedmicroscopic-f.php |
| work_keys_str_mv | AT mpavlik fungitoxicityofnaturalheterocycleglucosidevicineobtainedfromviciafabalagainstselectedmicroscopicfilamentousfungi AT mvanova fungitoxicityofnaturalheterocycleglucosidevicineobtainedfromviciafabalagainstselectedmicroscopicfilamentousfungi AT vlaudova fungitoxicityofnaturalheterocycleglucosidevicineobtainedfromviciafabalagainstselectedmicroscopicfilamentousfungi AT jharmatha fungitoxicityofnaturalheterocycleglucosidevicineobtainedfromviciafabalagainstselectedmicroscopicfilamentousfungi |