Effect of Polydeoxyribonucleotide on Lipopolysaccharide and Sevoflurane-Induced Postoperative Cognitive dysfunction in Human Neuronal SH-SY5Y Cells
Purpose Postoperative cognitive dysfunction (POCD) is a complication of surgery characterized by acute cognitive dysfunction, memory impairment, and loss of attention. The effect of polydeoxyribonucleotide (PDRN) on the POCD environment induced by lipopolysaccharide (LPS) and sevoflurane exposure we...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Continence Society
2019-11-01
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| Series: | International Neurourology Journal |
| Subjects: | |
| Online Access: | http://www.einj.org/upload/pdf/inj-1938218-109.pdf |
| _version_ | 1818359992247386112 |
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| author | Hyungmo Jeong Jun-Young Chung Il-Gyu Ko Sang-Hoon Kim Jun-Jang Jin Lakkyong Hwang Eun Jin Moon Bong Jae Lee Jae Woo Yi |
| author_facet | Hyungmo Jeong Jun-Young Chung Il-Gyu Ko Sang-Hoon Kim Jun-Jang Jin Lakkyong Hwang Eun Jin Moon Bong Jae Lee Jae Woo Yi |
| author_sort | Hyungmo Jeong |
| collection | DOAJ |
| description | Purpose Postoperative cognitive dysfunction (POCD) is a complication of surgery characterized by acute cognitive dysfunction, memory impairment, and loss of attention. The effect of polydeoxyribonucleotide (PDRN) on the POCD environment induced by lipopolysaccharide (LPS) and sevoflurane exposure were investigated in human neuronal SH-SY5Y cells. Methods The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and WST-8 assays were performed to determine cell viability. Cyclic adenosine-3,5′-monophosphate (cAMP), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 concentrations were measured using enzyme-linked immunoassay (ELISA). Immunocytochemistry was performed for vascular endothelial growth factor (VEGF) and brain-derived neurotrophic factor (BDNF), and western blotting for TNF-α, IL-1β, IL-6, and cAMP response element-binding protein (CREB). Results Induction of the POCD environment reduced cell viability in the MTT and WST-8 assays. PDRN treatment reduced TNF-α, IL-1β, and IL-6 expression in POCD conditions, and significantly increased cAMP concentrations and the p-CREB/CREB ratio. PDRN treatment activated adenosine A2A receptors and then increased the expression of VEGF and BDNF, which had been reduced by LPS and sevoflurane exposure. Conclusions PDRN treatment showed a therapeutic effect on the LPS and sevoflurane-induced POCD environment. PDRN was shown to have an excellent therapeutic effect on POCD, not only by promoting rapid anti-inflammatory effects in damaged cells, but also by enhancing the expression of BDNF and VEGF. |
| first_indexed | 2024-12-13T20:53:42Z |
| format | Article |
| id | doaj.art-ac7e7d15fc81483cb009a54525ede730 |
| institution | Directory Open Access Journal |
| issn | 2093-4777 2093-6931 |
| language | English |
| last_indexed | 2024-12-13T20:53:42Z |
| publishDate | 2019-11-01 |
| publisher | Korean Continence Society |
| record_format | Article |
| series | International Neurourology Journal |
| spelling | doaj.art-ac7e7d15fc81483cb009a54525ede7302022-12-21T23:31:49ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312019-11-0123Suppl 2S9310110.5213/inj.1938218.109820Effect of Polydeoxyribonucleotide on Lipopolysaccharide and Sevoflurane-Induced Postoperative Cognitive dysfunction in Human Neuronal SH-SY5Y CellsHyungmo Jeong0Jun-Young Chung1Il-Gyu Ko2Sang-Hoon Kim3Jun-Jang Jin4Lakkyong Hwang5Eun Jin Moon6Bong Jae Lee7Jae Woo Yi8 Department of Anesthesiology and Pain Medicine, Eutteum Hospital, Paju, Korea Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, KoreaPurpose Postoperative cognitive dysfunction (POCD) is a complication of surgery characterized by acute cognitive dysfunction, memory impairment, and loss of attention. The effect of polydeoxyribonucleotide (PDRN) on the POCD environment induced by lipopolysaccharide (LPS) and sevoflurane exposure were investigated in human neuronal SH-SY5Y cells. Methods The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and WST-8 assays were performed to determine cell viability. Cyclic adenosine-3,5′-monophosphate (cAMP), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 concentrations were measured using enzyme-linked immunoassay (ELISA). Immunocytochemistry was performed for vascular endothelial growth factor (VEGF) and brain-derived neurotrophic factor (BDNF), and western blotting for TNF-α, IL-1β, IL-6, and cAMP response element-binding protein (CREB). Results Induction of the POCD environment reduced cell viability in the MTT and WST-8 assays. PDRN treatment reduced TNF-α, IL-1β, and IL-6 expression in POCD conditions, and significantly increased cAMP concentrations and the p-CREB/CREB ratio. PDRN treatment activated adenosine A2A receptors and then increased the expression of VEGF and BDNF, which had been reduced by LPS and sevoflurane exposure. Conclusions PDRN treatment showed a therapeutic effect on the LPS and sevoflurane-induced POCD environment. PDRN was shown to have an excellent therapeutic effect on POCD, not only by promoting rapid anti-inflammatory effects in damaged cells, but also by enhancing the expression of BDNF and VEGF.http://www.einj.org/upload/pdf/inj-1938218-109.pdfpostoperative cognitive dysfunctionpolydeoxyribonucleotidespro-inflammatory cytokine |
| spellingShingle | Hyungmo Jeong Jun-Young Chung Il-Gyu Ko Sang-Hoon Kim Jun-Jang Jin Lakkyong Hwang Eun Jin Moon Bong Jae Lee Jae Woo Yi Effect of Polydeoxyribonucleotide on Lipopolysaccharide and Sevoflurane-Induced Postoperative Cognitive dysfunction in Human Neuronal SH-SY5Y Cells International Neurourology Journal postoperative cognitive dysfunction polydeoxyribonucleotides pro-inflammatory cytokine |
| title | Effect of Polydeoxyribonucleotide on Lipopolysaccharide and Sevoflurane-Induced Postoperative Cognitive dysfunction in Human Neuronal SH-SY5Y Cells |
| title_full | Effect of Polydeoxyribonucleotide on Lipopolysaccharide and Sevoflurane-Induced Postoperative Cognitive dysfunction in Human Neuronal SH-SY5Y Cells |
| title_fullStr | Effect of Polydeoxyribonucleotide on Lipopolysaccharide and Sevoflurane-Induced Postoperative Cognitive dysfunction in Human Neuronal SH-SY5Y Cells |
| title_full_unstemmed | Effect of Polydeoxyribonucleotide on Lipopolysaccharide and Sevoflurane-Induced Postoperative Cognitive dysfunction in Human Neuronal SH-SY5Y Cells |
| title_short | Effect of Polydeoxyribonucleotide on Lipopolysaccharide and Sevoflurane-Induced Postoperative Cognitive dysfunction in Human Neuronal SH-SY5Y Cells |
| title_sort | effect of polydeoxyribonucleotide on lipopolysaccharide and sevoflurane induced postoperative cognitive dysfunction in human neuronal sh sy5y cells |
| topic | postoperative cognitive dysfunction polydeoxyribonucleotides pro-inflammatory cytokine |
| url | http://www.einj.org/upload/pdf/inj-1938218-109.pdf |
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