Biosynthesized Iron Oxide Nanoparticles from <i>Petroselinum crispum</i> Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological Features
This study aimed to investigate the ameliorative effects of iron oxide nanoparticles (IONPs) prepared from leaf extract of <i>Petroselinum crispum</i> compared to those prepared using a chemical method in lead-acetate-induced anemic rats. Twenty rats were divided into four groups (five r...
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MDPI AG
2021-05-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/9/6/123 |
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| author | Sabry M. El-Bahr Amal M. Elbakery Nashwa El-Gazzar Aziza A. Amin Saad Al-Sultan Mohammed A. Alfattah Saad Shousha Sameer Alhojaily Mohammad Shathele Islam I. Sabeq Ahlam F. Hamouda |
| author_facet | Sabry M. El-Bahr Amal M. Elbakery Nashwa El-Gazzar Aziza A. Amin Saad Al-Sultan Mohammed A. Alfattah Saad Shousha Sameer Alhojaily Mohammad Shathele Islam I. Sabeq Ahlam F. Hamouda |
| author_sort | Sabry M. El-Bahr |
| collection | DOAJ |
| description | This study aimed to investigate the ameliorative effects of iron oxide nanoparticles (IONPs) prepared from leaf extract of <i>Petroselinum crispum</i> compared to those prepared using a chemical method in lead-acetate-induced anemic rats. Twenty rats were divided into four groups (five rats each). Throughout the experimental period (8 weeks), the rats in group 1 were not given any therapy. The rats in groups 2, 3 and 4 were given 400 ppm lead acetate orally for 2 weeks to make them anemic. Following that, these rats were either left untreated, given 27 ppm of chemical IONPs orally or given 27 ppm of natural IONPs orally for the remaining 6 weeks of the experiment. TEM analysis indicated that the chemically and naturally prepared IONPs had sizes of 6.22–9.7 and 64–68 nm, respectively. Serum ferritin and iron concentrations were reduced, whereas the total iron-binding capacity (TIBC), ALT, AST, urea and creatinine were significantly increased in the non-treated lead-acetate-induced anemic rats compared to those of the control. In addition, congestion, hemorrhage, necrosis, vacuolation and leukocytic infiltration in the kidneys, liver and spleen were observed in non-treated lead-acetate-induced anemic rats compared to the control. The effects of lead acetate were mitigated by IONPs, particularly the natural one. In conclusion, IONPs produced from <i>Petroselinum crispum</i> leaf extract can be used as an efficient and safe therapy in lead-acetate-induced anemic rats. |
| first_indexed | 2024-03-10T10:51:56Z |
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| language | English |
| last_indexed | 2024-03-10T10:51:56Z |
| publishDate | 2021-05-01 |
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| record_format | Article |
| series | Toxics |
| spelling | doaj.art-ac7f0a2f16154a7c8ed0a3bde32464c42023-11-21T22:10:50ZengMDPI AGToxics2305-63042021-05-019612310.3390/toxics9060123Biosynthesized Iron Oxide Nanoparticles from <i>Petroselinum crispum</i> Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological FeaturesSabry M. El-Bahr0Amal M. Elbakery1Nashwa El-Gazzar2Aziza A. Amin3Saad Al-Sultan4Mohammed A. Alfattah5Saad Shousha6Sameer Alhojaily7Mohammad Shathele8Islam I. Sabeq9Ahlam F. Hamouda10Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi ArabiaDepartment of Maize and Sugar Crop Disease Research, Plant Pathology Research Institute, ARC, Giza 12622, EgyptDepartment of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig 44519, EgyptDepartment of Histopathology, Faculty of Veterinary Medicine, Benha University, Benha 13736, EgyptDepartment of Public Health, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi ArabiaCamel Research Center, King Faisal University, Al-Ahsa 31982, Saudi ArabiaDepartment of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi ArabiaDepartment of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi ArabiaDepartment of Microbiology, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi ArabiaDepartment of Food Control and Hygiene, Faculty of Veterinary Medicine, Benha University, Benha 13736, EgyptDepartment of Forensic Medicine and Toxicology, Teaching Hospital, Faculty of Veterinary Medicine, Benha University, Benha 13736, EgyptThis study aimed to investigate the ameliorative effects of iron oxide nanoparticles (IONPs) prepared from leaf extract of <i>Petroselinum crispum</i> compared to those prepared using a chemical method in lead-acetate-induced anemic rats. Twenty rats were divided into four groups (five rats each). Throughout the experimental period (8 weeks), the rats in group 1 were not given any therapy. The rats in groups 2, 3 and 4 were given 400 ppm lead acetate orally for 2 weeks to make them anemic. Following that, these rats were either left untreated, given 27 ppm of chemical IONPs orally or given 27 ppm of natural IONPs orally for the remaining 6 weeks of the experiment. TEM analysis indicated that the chemically and naturally prepared IONPs had sizes of 6.22–9.7 and 64–68 nm, respectively. Serum ferritin and iron concentrations were reduced, whereas the total iron-binding capacity (TIBC), ALT, AST, urea and creatinine were significantly increased in the non-treated lead-acetate-induced anemic rats compared to those of the control. In addition, congestion, hemorrhage, necrosis, vacuolation and leukocytic infiltration in the kidneys, liver and spleen were observed in non-treated lead-acetate-induced anemic rats compared to the control. The effects of lead acetate were mitigated by IONPs, particularly the natural one. In conclusion, IONPs produced from <i>Petroselinum crispum</i> leaf extract can be used as an efficient and safe therapy in lead-acetate-induced anemic rats.https://www.mdpi.com/2305-6304/9/6/123lead acetateanemiananoparticles<i>Petroselinum crispum</i>rats |
| spellingShingle | Sabry M. El-Bahr Amal M. Elbakery Nashwa El-Gazzar Aziza A. Amin Saad Al-Sultan Mohammed A. Alfattah Saad Shousha Sameer Alhojaily Mohammad Shathele Islam I. Sabeq Ahlam F. Hamouda Biosynthesized Iron Oxide Nanoparticles from <i>Petroselinum crispum</i> Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological Features Toxics lead acetate anemia nanoparticles <i>Petroselinum crispum</i> rats |
| title | Biosynthesized Iron Oxide Nanoparticles from <i>Petroselinum crispum</i> Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological Features |
| title_full | Biosynthesized Iron Oxide Nanoparticles from <i>Petroselinum crispum</i> Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological Features |
| title_fullStr | Biosynthesized Iron Oxide Nanoparticles from <i>Petroselinum crispum</i> Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological Features |
| title_full_unstemmed | Biosynthesized Iron Oxide Nanoparticles from <i>Petroselinum crispum</i> Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological Features |
| title_short | Biosynthesized Iron Oxide Nanoparticles from <i>Petroselinum crispum</i> Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological Features |
| title_sort | biosynthesized iron oxide nanoparticles from i petroselinum crispum i leaf extract mitigate lead acetate induced anemia in male albino rats hematological biochemical and histopathological features |
| topic | lead acetate anemia nanoparticles <i>Petroselinum crispum</i> rats |
| url | https://www.mdpi.com/2305-6304/9/6/123 |
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