A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and Potency
It is generally accepted that citrate or the A-form of acid-citrate-dextrose (ACD-A) are suitable for preparing platelet-rich plasma (PRP) for regenerative therapy. However, this is based on evidence from blood transfusions and not from regenerative medicine. Thus, we examined the effects of anticoa...
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MDPI AG
2020-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/3/42 |
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| author | Hachidai Aizawa Hideo Kawabata Atsushi Sato Hideo Masuki Taisuke Watanabe Tetsuhiro Tsujino Kazushige Isobe Masayuki Nakamura Koh Nakata Tomoyuki Kawase |
| author_facet | Hachidai Aizawa Hideo Kawabata Atsushi Sato Hideo Masuki Taisuke Watanabe Tetsuhiro Tsujino Kazushige Isobe Masayuki Nakamura Koh Nakata Tomoyuki Kawase |
| author_sort | Hachidai Aizawa |
| collection | DOAJ |
| description | It is generally accepted that citrate or the A-form of acid-citrate-dextrose (ACD-A) are suitable for preparing platelet-rich plasma (PRP) for regenerative therapy. However, this is based on evidence from blood transfusions and not from regenerative medicine. Thus, we examined the effects of anticoagulants, such as ACD-A, ethylenediaminetetraacetic acid (EDTA), and heparin, on the regenerative quality of PRP to address this gap. The blood samples were collected in the presence of anticoagulants and were processed to prepare pure-PRP. Platelet size, activation status, and intra-platelet free Ca<sup>2+</sup> concentration were determined while using a hematology analyzer and flow cytometer. Platelet-derived growth factor-BB (PDGF-BB) was quantified while using an ELISA. In pure-PRP samples, EDTA caused platelet swelling and activation, but yielded the highest number of platelets. Heparin aggregated platelets and disturbed the overall counting of blood cells. However, no significant differences in PDGF-BB levels were observed among the anticoagulants tested. Moreover, when considering the easy preparation of platelet suspensions, without the need for high-level pipetting skills, these findings suggest the comparable potency of EDTA-derived pure-PRP in tissue regeneration and support the use of EDTA in the preparation of pure-PRP. Further in vivo studies are required in animal models to exclude the possible negative effects of including EDTA in pure-PRP preparations. |
| first_indexed | 2024-12-21T10:47:41Z |
| format | Article |
| id | doaj.art-ac7f6c4160dd48ac923a76da4ce70043 |
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| language | English |
| last_indexed | 2024-12-21T10:47:41Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-ac7f6c4160dd48ac923a76da4ce700432022-12-21T19:06:45ZengMDPI AGBiomedicines2227-90592020-02-01834210.3390/biomedicines8030042biomedicines8030042A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and PotencyHachidai Aizawa0Hideo Kawabata1Atsushi Sato2Hideo Masuki3Taisuke Watanabe4Tetsuhiro Tsujino5Kazushige Isobe6Masayuki Nakamura7Koh Nakata8Tomoyuki Kawase9Tokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanBioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, JapanDivision of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, JapanIt is generally accepted that citrate or the A-form of acid-citrate-dextrose (ACD-A) are suitable for preparing platelet-rich plasma (PRP) for regenerative therapy. However, this is based on evidence from blood transfusions and not from regenerative medicine. Thus, we examined the effects of anticoagulants, such as ACD-A, ethylenediaminetetraacetic acid (EDTA), and heparin, on the regenerative quality of PRP to address this gap. The blood samples were collected in the presence of anticoagulants and were processed to prepare pure-PRP. Platelet size, activation status, and intra-platelet free Ca<sup>2+</sup> concentration were determined while using a hematology analyzer and flow cytometer. Platelet-derived growth factor-BB (PDGF-BB) was quantified while using an ELISA. In pure-PRP samples, EDTA caused platelet swelling and activation, but yielded the highest number of platelets. Heparin aggregated platelets and disturbed the overall counting of blood cells. However, no significant differences in PDGF-BB levels were observed among the anticoagulants tested. Moreover, when considering the easy preparation of platelet suspensions, without the need for high-level pipetting skills, these findings suggest the comparable potency of EDTA-derived pure-PRP in tissue regeneration and support the use of EDTA in the preparation of pure-PRP. Further in vivo studies are required in animal models to exclude the possible negative effects of including EDTA in pure-PRP preparations.https://www.mdpi.com/2227-9059/8/3/42anticoagulantsedtaacd-aheparinplateletsplatelet-rich plasmapdgf |
| spellingShingle | Hachidai Aizawa Hideo Kawabata Atsushi Sato Hideo Masuki Taisuke Watanabe Tetsuhiro Tsujino Kazushige Isobe Masayuki Nakamura Koh Nakata Tomoyuki Kawase A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and Potency Biomedicines anticoagulants edta acd-a heparin platelets platelet-rich plasma pdgf |
| title | A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and Potency |
| title_full | A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and Potency |
| title_fullStr | A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and Potency |
| title_full_unstemmed | A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and Potency |
| title_short | A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and Potency |
| title_sort | comparative study of the effects of anticoagulants on pure platelet rich plasma quality and potency |
| topic | anticoagulants edta acd-a heparin platelets platelet-rich plasma pdgf |
| url | https://www.mdpi.com/2227-9059/8/3/42 |
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