A Comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, AGS, and PC3 Cancer Cell Lines
Background: All natural anticancer agents are cytotoxic basically and act mainly by the inhibition cell proliferation; but they have different mechanisms. Two assays, thiazolyl blue [3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyl-terazoliumbromide or MTT] and sulforhodamine B (SRB), are used to asses...
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| Format: | Article |
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Isfahan University of Medical Sciences
2017-10-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/8426 |
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| author | Farzaneh Raeisi Elham Raeisi Daryoush Shahbazi-Gahrouei Esfandiar Heidarian Fatemeh Amini |
| author_facet | Farzaneh Raeisi Elham Raeisi Daryoush Shahbazi-Gahrouei Esfandiar Heidarian Fatemeh Amini |
| author_sort | Farzaneh Raeisi |
| collection | DOAJ |
| description | Background: All natural anticancer agents are cytotoxic basically and act mainly by the inhibition cell proliferation; but they have different mechanisms. Two assays, thiazolyl blue [3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyl-terazoliumbromide or MTT] and sulforhodamine B (SRB), are used to assess cell growth. This study aimed to compare measurements between MTT and SRB on the cancer cell lines.
Methods: Different concentrations of the bromelain were added to cultured cells including mouse breast cancer (4T1), human gastric carcinoma (AGS), and human prostate carcinoma (PC3) cell lines and incubated at 24 and 48 hours. The growth and proliferation rates of the studied cells were investigated using both MTT and SRB assays after treatment with bromelain. The differences between cells were determined using Kruskal-Wallis and Dunns tests.
Findings: Bromelain significantly decreased growth and proliferation rate of 4T1, AGS and PC3 cancer cells, in a concentration-dependent manner at different times, in both MTT and SRB assays.
Conclusion: Findings showed that both MTT and SRB assays gained similar data regardless of the cell types. |
| first_indexed | 2024-03-12T06:50:26Z |
| format | Article |
| id | doaj.art-ac8056186f9a40daa5f5f73357d2978b |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T06:50:26Z |
| publishDate | 2017-10-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-ac8056186f9a40daa5f5f73357d2978b2023-09-03T00:20:03ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2017-10-0135443105610612617A Comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, AGS, and PC3 Cancer Cell LinesFarzaneh Raeisi0Elham Raeisi1Daryoush Shahbazi-Gahrouei2Esfandiar Heidarian3Fatemeh Amini4Researcher, Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranAssistant Professor, Clinical Biochemistry Research Center, Basic Health Sciences Institute AND Department of Medical Physics and Radiology, School of Allied Medical Sciences, Shahrekord University of Medical Sciences, Shahrekord, IranProfessor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranProfessor, Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranMSc Student, Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, IranBackground: All natural anticancer agents are cytotoxic basically and act mainly by the inhibition cell proliferation; but they have different mechanisms. Two assays, thiazolyl blue [3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyl-terazoliumbromide or MTT] and sulforhodamine B (SRB), are used to assess cell growth. This study aimed to compare measurements between MTT and SRB on the cancer cell lines. Methods: Different concentrations of the bromelain were added to cultured cells including mouse breast cancer (4T1), human gastric carcinoma (AGS), and human prostate carcinoma (PC3) cell lines and incubated at 24 and 48 hours. The growth and proliferation rates of the studied cells were investigated using both MTT and SRB assays after treatment with bromelain. The differences between cells were determined using Kruskal-Wallis and Dunns tests. Findings: Bromelain significantly decreased growth and proliferation rate of 4T1, AGS and PC3 cancer cells, in a concentration-dependent manner at different times, in both MTT and SRB assays. Conclusion: Findings showed that both MTT and SRB assays gained similar data regardless of the cell types.http://jims.mui.ac.ir/index.php/jims/article/view/8426BromelainNeoplasmsCell proliferationThiazolyl blue, Sulforhodamine B |
| spellingShingle | Farzaneh Raeisi Elham Raeisi Daryoush Shahbazi-Gahrouei Esfandiar Heidarian Fatemeh Amini A Comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, AGS, and PC3 Cancer Cell Lines مجله دانشکده پزشکی اصفهان Bromelain Neoplasms Cell proliferation Thiazolyl blue, Sulforhodamine B |
| title | A Comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, AGS, and PC3 Cancer Cell Lines |
| title_full | A Comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, AGS, and PC3 Cancer Cell Lines |
| title_fullStr | A Comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, AGS, and PC3 Cancer Cell Lines |
| title_full_unstemmed | A Comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, AGS, and PC3 Cancer Cell Lines |
| title_short | A Comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) Assay in Assessment of Antiproliferative Effect of Bromelain on 4T1, AGS, and PC3 Cancer Cell Lines |
| title_sort | comparison of thiazolyl blue mtt versus sulforhodamine b srb assay in assessment of antiproliferative effect of bromelain on 4t1 ags and pc3 cancer cell lines |
| topic | Bromelain Neoplasms Cell proliferation Thiazolyl blue, Sulforhodamine B |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/8426 |
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