Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway
Polycystic ovary syndrome (PCOS) is recognized as a general endocrine disease and reproductive disorder. Although evidence indicates that PCOS has a complex etiology and genetic basis, the pathogenic mechanisms and signal pathway in PCOS remain unclear. In this study, the normal structure of follicl...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-07-01
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| Series: | Animal Cells and Systems |
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| Online Access: | http://dx.doi.org/10.1080/19768354.2020.1790416 |
| _version_ | 1818996852431781888 |
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| author | Zhaowei Cai Shaojuan He Tao Li Li Zhao Kerong Zhang |
| author_facet | Zhaowei Cai Shaojuan He Tao Li Li Zhao Kerong Zhang |
| author_sort | Zhaowei Cai |
| collection | DOAJ |
| description | Polycystic ovary syndrome (PCOS) is recognized as a general endocrine disease and reproductive disorder. Although evidence indicates that PCOS has a complex etiology and genetic basis, the pathogenic mechanisms and signal pathway in PCOS remain unclear. In this study, the normal structure of follicle and corpus luteum were observed, and no cyst nor hyperemia was observed under the light microscopic study with hematoxylin and eosin (H&E) staining. Eestosterone and progesterone were evaluated by radioimmunoassay in rat serum. The alterations of proliferative ability and cell cycle distribution of each group were assessed by Cell Counting Kit-8 (CCK8) assay and flow cytometry. The protein expression of p-mTOR/mTOR, p-PI3K/PI3K, p-AKT/AKT, and GAPDH were analyzed by western blotting. Both doses of PLB could benefit the ovarian morphology and polycystic property. PLBinduced a suppress effect on the proliferation of rat ovarian granulosa cells. In addition, PLB also induced concentration-dependent apoptosis in rat ovarian granulosa cells. The rat ovarian granulosa cells treated with PLB that the expression levels of p-AKT, p-mTOR, and p-PI3K were significantly decreased in a concentration-dependent manner. PLB not only plays a critical role in attenuating the pathology and polycystic property changes in the ovary but can also induce rat ovarian granulosa cell apoptosis through the PI3K/Akt/mTOR signal pathway. This study showed the innovative role of PLB in the pathogenesis of PCOS and provides a new therapeutic modality for the treatment of PCOS. |
| first_indexed | 2024-12-20T21:36:19Z |
| format | Article |
| id | doaj.art-ac83143ce68246bbb9500a751039d116 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-12-20T21:36:19Z |
| publishDate | 2020-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-ac83143ce68246bbb9500a751039d1162022-12-21T19:25:56ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852020-07-0124419720410.1080/19768354.2020.17904161790416Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathwayZhaowei Cai0Shaojuan He1Tao Li2Li Zhao3Kerong Zhang4Reproductive Center, SSL Central Hospital of Dongguan CityDepartment of Clinical Laboratory, Dongguan People’s HospitalGuangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical UniversityReproductive Center, SSL Central Hospital of Dongguan CityDepartment of Gynaecology and Obstetrics and Reproductive Medicine, Second Clinical Medical College of Guangdong Medical, Guangdong Medical UniversityPolycystic ovary syndrome (PCOS) is recognized as a general endocrine disease and reproductive disorder. Although evidence indicates that PCOS has a complex etiology and genetic basis, the pathogenic mechanisms and signal pathway in PCOS remain unclear. In this study, the normal structure of follicle and corpus luteum were observed, and no cyst nor hyperemia was observed under the light microscopic study with hematoxylin and eosin (H&E) staining. Eestosterone and progesterone were evaluated by radioimmunoassay in rat serum. The alterations of proliferative ability and cell cycle distribution of each group were assessed by Cell Counting Kit-8 (CCK8) assay and flow cytometry. The protein expression of p-mTOR/mTOR, p-PI3K/PI3K, p-AKT/AKT, and GAPDH were analyzed by western blotting. Both doses of PLB could benefit the ovarian morphology and polycystic property. PLBinduced a suppress effect on the proliferation of rat ovarian granulosa cells. In addition, PLB also induced concentration-dependent apoptosis in rat ovarian granulosa cells. The rat ovarian granulosa cells treated with PLB that the expression levels of p-AKT, p-mTOR, and p-PI3K were significantly decreased in a concentration-dependent manner. PLB not only plays a critical role in attenuating the pathology and polycystic property changes in the ovary but can also induce rat ovarian granulosa cell apoptosis through the PI3K/Akt/mTOR signal pathway. This study showed the innovative role of PLB in the pathogenesis of PCOS and provides a new therapeutic modality for the treatment of PCOS.http://dx.doi.org/10.1080/19768354.2020.1790416pi3k/akt/mtor signal pathwaypolycystic ovary syndromeplumbaginapoptosisovarian granulosa cells |
| spellingShingle | Zhaowei Cai Shaojuan He Tao Li Li Zhao Kerong Zhang Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway Animal Cells and Systems pi3k/akt/mtor signal pathway polycystic ovary syndrome plumbagin apoptosis ovarian granulosa cells |
| title | Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway |
| title_full | Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway |
| title_fullStr | Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway |
| title_full_unstemmed | Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway |
| title_short | Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway |
| title_sort | plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating pi3k akt mtor pathway |
| topic | pi3k/akt/mtor signal pathway polycystic ovary syndrome plumbagin apoptosis ovarian granulosa cells |
| url | http://dx.doi.org/10.1080/19768354.2020.1790416 |
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