Evaluation of a commercial interferon-γ release assay for the detection of SARS-CoV-2 T-cell response after vaccination
Objective: Evidence regarding the role of cellular immunity in protecting against COVID-19 is emerging. To better assess immune status, simple and robust assays measuring specific T-cell responses associated with humoral responses are needed. We aimed to evaluate the Quan-T-Cell SARS-CoV-2 test for...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023043943 |
| _version_ | 1797804612677271552 |
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| author | Imane Saad Albichr Samy Mzougui Arnaud Devresse Hélène Georgery Eric Goffin Nada Kanaan Jean Cyr Yombi Leila Belkhir Julien De Greef Anaïs Scohy Hector Rodriguez-Villalobos Benoît Kabamba-Mukadi |
| author_facet | Imane Saad Albichr Samy Mzougui Arnaud Devresse Hélène Georgery Eric Goffin Nada Kanaan Jean Cyr Yombi Leila Belkhir Julien De Greef Anaïs Scohy Hector Rodriguez-Villalobos Benoît Kabamba-Mukadi |
| author_sort | Imane Saad Albichr |
| collection | DOAJ |
| description | Objective: Evidence regarding the role of cellular immunity in protecting against COVID-19 is emerging. To better assess immune status, simple and robust assays measuring specific T-cell responses associated with humoral responses are needed. We aimed to evaluate the Quan-T-Cell SARS-CoV-2 test for measuring cellular immune responses in vaccinated healthy and immunosuppressed subjects. Methods: T-cell responses were assessed in healthy vaccinated and unvaccinated and unexposed healthcare workers to determine the sensitivity and specificity of the EUROIMMUN SARS-CoV-2 Quan-T-Cell IGRA test performed on vaccinated kidney transplant recipients (KTRs). Results: The EUROIMMUN SARS-CoV-2 Quan-T-Cell IGRA test showed good sensitivity (87.2%) and specificity (92.3%) at the calculated 147 mIU/mL cutoff, with an 88.33% accuracy. In KTRs, specific cellular immunity was lower than the antibody response; however, those with a positive IGRA result produced as much IFN-γ as healthy individuals. Conclusions: The EUROIMMUN SARS-CoV-2 Quan-T-Cell IGRA test showed good sensitivity and specificity for the detection of specific T-cell responses against the SARS-CoV-2 spike protein. These results present an additional tool for better management of COVID-19, especially in vulnerable populations. |
| first_indexed | 2024-03-13T05:39:49Z |
| format | Article |
| id | doaj.art-ac8612d11e294f3cb17d5c2ac8dac82b |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T05:39:49Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-ac8612d11e294f3cb17d5c2ac8dac82b2023-06-14T04:33:55ZengElsevierHeliyon2405-84402023-06-0196e17186Evaluation of a commercial interferon-γ release assay for the detection of SARS-CoV-2 T-cell response after vaccinationImane Saad Albichr0Samy Mzougui1Arnaud Devresse2Hélène Georgery3Eric Goffin4Nada Kanaan5Jean Cyr Yombi6Leila Belkhir7Julien De Greef8Anaïs Scohy9Hector Rodriguez-Villalobos10Benoît Kabamba-Mukadi11Department of Microbiology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, Belgium; Corresponding author.Department of Microbiology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumDepartment of Nephrology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, Belgium; Institut de Recherche Clinique et Expérimentale, Université Catholique de Louvain, Brussels, BelgiumDepartment of Nephrology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumDepartment of Nephrology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumDepartment of Nephrology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumDepartment of Internal Medicine and Infectious Disease, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumDepartment of Internal Medicine and Infectious Disease, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumDepartment of Internal Medicine and Infectious Disease, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumDepartment of Microbiology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumDepartment of Microbiology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumDepartment of Microbiology, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, BelgiumObjective: Evidence regarding the role of cellular immunity in protecting against COVID-19 is emerging. To better assess immune status, simple and robust assays measuring specific T-cell responses associated with humoral responses are needed. We aimed to evaluate the Quan-T-Cell SARS-CoV-2 test for measuring cellular immune responses in vaccinated healthy and immunosuppressed subjects. Methods: T-cell responses were assessed in healthy vaccinated and unvaccinated and unexposed healthcare workers to determine the sensitivity and specificity of the EUROIMMUN SARS-CoV-2 Quan-T-Cell IGRA test performed on vaccinated kidney transplant recipients (KTRs). Results: The EUROIMMUN SARS-CoV-2 Quan-T-Cell IGRA test showed good sensitivity (87.2%) and specificity (92.3%) at the calculated 147 mIU/mL cutoff, with an 88.33% accuracy. In KTRs, specific cellular immunity was lower than the antibody response; however, those with a positive IGRA result produced as much IFN-γ as healthy individuals. Conclusions: The EUROIMMUN SARS-CoV-2 Quan-T-Cell IGRA test showed good sensitivity and specificity for the detection of specific T-cell responses against the SARS-CoV-2 spike protein. These results present an additional tool for better management of COVID-19, especially in vulnerable populations.http://www.sciencedirect.com/science/article/pii/S2405844023043943COVID-19SARS-CoV-2VaccineCellular immunityInterferon-gamma release tests |
| spellingShingle | Imane Saad Albichr Samy Mzougui Arnaud Devresse Hélène Georgery Eric Goffin Nada Kanaan Jean Cyr Yombi Leila Belkhir Julien De Greef Anaïs Scohy Hector Rodriguez-Villalobos Benoît Kabamba-Mukadi Evaluation of a commercial interferon-γ release assay for the detection of SARS-CoV-2 T-cell response after vaccination Heliyon COVID-19 SARS-CoV-2 Vaccine Cellular immunity Interferon-gamma release tests |
| title | Evaluation of a commercial interferon-γ release assay for the detection of SARS-CoV-2 T-cell response after vaccination |
| title_full | Evaluation of a commercial interferon-γ release assay for the detection of SARS-CoV-2 T-cell response after vaccination |
| title_fullStr | Evaluation of a commercial interferon-γ release assay for the detection of SARS-CoV-2 T-cell response after vaccination |
| title_full_unstemmed | Evaluation of a commercial interferon-γ release assay for the detection of SARS-CoV-2 T-cell response after vaccination |
| title_short | Evaluation of a commercial interferon-γ release assay for the detection of SARS-CoV-2 T-cell response after vaccination |
| title_sort | evaluation of a commercial interferon γ release assay for the detection of sars cov 2 t cell response after vaccination |
| topic | COVID-19 SARS-CoV-2 Vaccine Cellular immunity Interferon-gamma release tests |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023043943 |
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