Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction
Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-09-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/12/9/4851 |
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| author | Pierre Saboural Frédéric Chaubet Francois Rouzet Faisal Al-Shoukr Rana Ben Azzouna Nadia Bouchemal Luc Picton Liliane Louedec Murielle Maire Lydia Rolland Guy Potier Dominique Le Guludec Didier Letourneur Cédric Chauvierre |
| author_facet | Pierre Saboural Frédéric Chaubet Francois Rouzet Faisal Al-Shoukr Rana Ben Azzouna Nadia Bouchemal Luc Picton Liliane Louedec Murielle Maire Lydia Rolland Guy Potier Dominique Le Guludec Didier Letourneur Cédric Chauvierre |
| author_sort | Pierre Saboural |
| collection | DOAJ |
| description | Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with 99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome. |
| first_indexed | 2024-04-11T13:17:24Z |
| format | Article |
| id | doaj.art-ac8754790b0d4dba880ac02005549cb8 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:17:24Z |
| publishDate | 2014-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-ac8754790b0d4dba880ac02005549cb82022-12-22T04:22:23ZengMDPI AGMarine Drugs1660-33972014-09-011294851486710.3390/md12094851md12094851Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial InfarctionPierre Saboural0Frédéric Chaubet1Francois Rouzet2Faisal Al-Shoukr3Rana Ben Azzouna4Nadia Bouchemal5Luc Picton6Liliane Louedec7Murielle Maire8Lydia Rolland9Guy Potier10Dominique Le Guludec11Didier Letourneur12Cédric Chauvierre13Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceInserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceInserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceInserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceInserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceLaboratory CSPBAT, Paris 13 University, Sorbonne Paris Cité, CNRS UMR 7244, SBMB team, F-93017, Bobigny, FranceLaboratory of Polymers Biopolymers Surfaces, Normandie University, Rouen University, F-76821, Mont Saint Aignan, FranceInserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceInserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceAlgues & Mer, Kernigou, F-29242, Ouessant, FranceAlgues & Mer, Kernigou, F-29242, Ouessant, FranceInserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceInserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceInserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, FranceFucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with 99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome.http://www.mdpi.com/1660-3397/12/9/4851fucoidanmolecular imagingatherothrombosisSPECT |
| spellingShingle | Pierre Saboural Frédéric Chaubet Francois Rouzet Faisal Al-Shoukr Rana Ben Azzouna Nadia Bouchemal Luc Picton Liliane Louedec Murielle Maire Lydia Rolland Guy Potier Dominique Le Guludec Didier Letourneur Cédric Chauvierre Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction Marine Drugs fucoidan molecular imaging atherothrombosis SPECT |
| title | Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction |
| title_full | Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction |
| title_fullStr | Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction |
| title_full_unstemmed | Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction |
| title_short | Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction |
| title_sort | purification of a low molecular weight fucoidan for spect molecular imaging of myocardial infarction |
| topic | fucoidan molecular imaging atherothrombosis SPECT |
| url | http://www.mdpi.com/1660-3397/12/9/4851 |
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