Analysis of Genetic Variability of Austrian Pine (Pinus nigra Arnold) in Serbia Using Protein Markers
Background and Purpose: The aim of present research is to study intra- and inter-population genetic variation in Austrian pine by the application of biochemical markers. A better knowledge of the genetic potential of Austrian pine populations will enhance the production of seed and planting material...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Croatian Forest Research Institute
2012-06-01
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| Series: | South-East European Forestry |
| Subjects: | |
| Online Access: | http://www.seefor.eu/archive/2012/vol-3-no-1/82-vol3-no1-lucic-et-al-analysis-of-genetic-variability-of-austrian-pine-pinus-nigra-arnold-in-serbia-using-protein-markers.html |
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| author | Aleksandar Lučić Vasilije Isajev Ljubinko Rakonjac Vladan Popović Radovan Nevenić Tatjana Ćirković-Mitrović Ljiljana Brašanac-Bosanac |
| author_facet | Aleksandar Lučić Vasilije Isajev Ljubinko Rakonjac Vladan Popović Radovan Nevenić Tatjana Ćirković-Mitrović Ljiljana Brašanac-Bosanac |
| author_sort | Aleksandar Lučić |
| collection | DOAJ |
| description | Background and Purpose: The aim of present research is to study intra- and inter-population genetic variation in Austrian pine by the application of biochemical markers. A better knowledge of the genetic potential of Austrian pine populations will enhance the production of seed and planting material, and in this way also the success of afforestation and the establishment of Austrian pine specific-purpose plantations.
Material and Methods: The polymorphism of protein markers was determined based on the selected genotypes originating from six populations (seed stands) in Serbia. Based on the derived electrophoregrams, qualitative and quantitative differences (number and pattern) in protein fractions were identified and the seed protein profile was constructed for each tree, as well as for each of the six study populations. Analysis of protein markers was performed using two statistical methods, NTSYS and correspondence analysis.
Results and Conclusion: The conservativeness of some polypeptide, i.e. the presence in all analysed genotypes is showed. On the other hand, some protein fractions were variable at the population level, and some were variable depending on the population. The degree of genetic variation among the populations was higher than the variation within the populations. There was also a high genetic variation in seed proteins within the analysed populations. Both methods (NTSYS and correspondence analysis) give the same arrangement of the analyzed populations, whereby, because of a different view of genetic distances, they can and should be combined, enabling easier and more precise understanding of mutual relationships of the observation units. |
| first_indexed | 2024-12-13T14:27:14Z |
| format | Article |
| id | doaj.art-ac87dcf28de3451d9ef4e8a39957ce80 |
| institution | Directory Open Access Journal |
| issn | 1847-6481 1849-0891 |
| language | English |
| last_indexed | 2024-12-13T14:27:14Z |
| publishDate | 2012-06-01 |
| publisher | Croatian Forest Research Institute |
| record_format | Article |
| series | South-East European Forestry |
| spelling | doaj.art-ac87dcf28de3451d9ef4e8a39957ce802022-12-21T23:41:55ZengCroatian Forest Research InstituteSouth-East European Forestry1847-64811849-08912012-06-013131010.15177/seefor.12-01 Analysis of Genetic Variability of Austrian Pine (Pinus nigra Arnold) in Serbia Using Protein MarkersAleksandar Lučić0Vasilije Isajev1Ljubinko Rakonjac2Vladan Popović3Radovan Nevenić4Tatjana Ćirković-Mitrović5Ljiljana Brašanac-Bosanac6Institute of Forestry, Kneza Viseslava 3, 11000 Belgrade, SerbiaUniversity of Belgrade, Faculty of Forestry, Kneza Viseslava 1, 11000 Belgrade, SerbiaInstitute of Forestry, Kneza Viseslava 3, 11000 Belgrade, SerbiaInstitute of Forestry, Kneza Viseslava 3, 11000 Belgrade, SerbiaInstitute of Forestry, Kneza Viseslava 3, 11000 Belgrade, SerbiaInstitute of Forestry, Kneza Viseslava 3, 11000 Belgrade, SerbiaInstitute of Forestry, Kneza Viseslava 3, 11000 Belgrade, SerbiaBackground and Purpose: The aim of present research is to study intra- and inter-population genetic variation in Austrian pine by the application of biochemical markers. A better knowledge of the genetic potential of Austrian pine populations will enhance the production of seed and planting material, and in this way also the success of afforestation and the establishment of Austrian pine specific-purpose plantations. Material and Methods: The polymorphism of protein markers was determined based on the selected genotypes originating from six populations (seed stands) in Serbia. Based on the derived electrophoregrams, qualitative and quantitative differences (number and pattern) in protein fractions were identified and the seed protein profile was constructed for each tree, as well as for each of the six study populations. Analysis of protein markers was performed using two statistical methods, NTSYS and correspondence analysis. Results and Conclusion: The conservativeness of some polypeptide, i.e. the presence in all analysed genotypes is showed. On the other hand, some protein fractions were variable at the population level, and some were variable depending on the population. The degree of genetic variation among the populations was higher than the variation within the populations. There was also a high genetic variation in seed proteins within the analysed populations. Both methods (NTSYS and correspondence analysis) give the same arrangement of the analyzed populations, whereby, because of a different view of genetic distances, they can and should be combined, enabling easier and more precise understanding of mutual relationships of the observation units.http://www.seefor.eu/archive/2012/vol-3-no-1/82-vol3-no1-lucic-et-al-analysis-of-genetic-variability-of-austrian-pine-pinus-nigra-arnold-in-serbia-using-protein-markers.htmlAustrian pineproteinspolymorphism |
| spellingShingle | Aleksandar Lučić Vasilije Isajev Ljubinko Rakonjac Vladan Popović Radovan Nevenić Tatjana Ćirković-Mitrović Ljiljana Brašanac-Bosanac Analysis of Genetic Variability of Austrian Pine (Pinus nigra Arnold) in Serbia Using Protein Markers South-East European Forestry Austrian pine proteins polymorphism |
| title | Analysis of Genetic Variability of Austrian Pine (Pinus nigra Arnold) in Serbia Using Protein Markers |
| title_full | Analysis of Genetic Variability of Austrian Pine (Pinus nigra Arnold) in Serbia Using Protein Markers |
| title_fullStr | Analysis of Genetic Variability of Austrian Pine (Pinus nigra Arnold) in Serbia Using Protein Markers |
| title_full_unstemmed | Analysis of Genetic Variability of Austrian Pine (Pinus nigra Arnold) in Serbia Using Protein Markers |
| title_short | Analysis of Genetic Variability of Austrian Pine (Pinus nigra Arnold) in Serbia Using Protein Markers |
| title_sort | analysis of genetic variability of austrian pine pinus nigra arnold in serbia using protein markers |
| topic | Austrian pine proteins polymorphism |
| url | http://www.seefor.eu/archive/2012/vol-3-no-1/82-vol3-no1-lucic-et-al-analysis-of-genetic-variability-of-austrian-pine-pinus-nigra-arnold-in-serbia-using-protein-markers.html |
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