Identification of MicroRNAs in Helicoverpa armigera and Spodoptera litura Based on Deep Sequencing and Homology Analysis
<p>The current identification of microRNAs (miRNAs) in insects is largely dependent on genome sequences. However, the lack of available genome sequences inhibits the identification of miRNAs in various insect species. In this study, we used a miRNA database of the silkworm <i>Bombyx mori...
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2013-01-01
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| Series: | International Journal of Biological Sciences |
| Online Access: | http://www.biolsci.org/v09p0001.htm |
| _version_ | 1811242723004383232 |
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| author | Xie Ge, Yong Zhang, Jianhao Jiang, Yi Zhong, Xiaonan Yang, Zhiqian Li, Yongping Huang, Anjiang Tan |
| author_facet | Xie Ge, Yong Zhang, Jianhao Jiang, Yi Zhong, Xiaonan Yang, Zhiqian Li, Yongping Huang, Anjiang Tan |
| author_sort | Xie Ge, Yong Zhang, Jianhao Jiang, Yi Zhong, Xiaonan Yang, Zhiqian Li, Yongping Huang, Anjiang Tan |
| collection | DOAJ |
| description | <p>The current identification of microRNAs (miRNAs) in insects is largely dependent on genome sequences. However, the lack of available genome sequences inhibits the identification of miRNAs in various insect species. In this study, we used a miRNA database of the silkworm <i>Bombyx mori</i> as a reference to identify miRNAs in <i>Helicoverpa armigera</i> and <i>Spodoptera litura</i> using deep sequencing and homology analysis. Because all three species belong to the Lepidoptera, the experiment produced reliable results. Our study identified 97 and 91 conserved miRNAs in <i>H. armigera</i> and <i>S. litura</i>, respectively. Using the genome of <i>B. mori</i> and BAC sequences of <i>H. armigera</i> as references, 1 novel miRNA and 8 novel miRNA candidates were identified in <i>H. armigera</i>, and 4 novel miRNA candidates were identified in <i>S. litura</i>. An evolutionary analysis revealed that most of the identified miRNAs were insect-specific, and more than 20 miRNAs were Lepidoptera-specific. The investigation of the expression patterns of <i>miR-2a</i>, <i>miR-34</i>, <i>miR-2796-3p</i> and <i>miR-11</i> revealed their potential roles in insect development. miRNA target prediction revealed that conserved miRNA target sites exist in various genes in the 3 species. Conserved miRNA target sites for the <i>Hsp90</i> gene among the 3 species were validated in the mammalian 293T cell line using a dual-luciferase reporter assay. Our study provides a new approach with which to identify miRNAs in insects lacking genome information and contributes to the functional analysis of insect miRNAs.</p> |
| first_indexed | 2024-04-12T13:55:02Z |
| format | Article |
| id | doaj.art-ac8cd8884d7241a48b8251b07decd64f |
| institution | Directory Open Access Journal |
| issn | 1449-2288 |
| language | English |
| last_indexed | 2024-04-12T13:55:02Z |
| publishDate | 2013-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Biological Sciences |
| spelling | doaj.art-ac8cd8884d7241a48b8251b07decd64f2022-12-22T03:30:24ZengIvyspring International PublisherInternational Journal of Biological Sciences1449-22882013-01-0191115Identification of MicroRNAs in Helicoverpa armigera and Spodoptera litura Based on Deep Sequencing and Homology AnalysisXie Ge, Yong Zhang, Jianhao Jiang, Yi Zhong, Xiaonan Yang, Zhiqian Li, Yongping Huang, Anjiang Tan<p>The current identification of microRNAs (miRNAs) in insects is largely dependent on genome sequences. However, the lack of available genome sequences inhibits the identification of miRNAs in various insect species. In this study, we used a miRNA database of the silkworm <i>Bombyx mori</i> as a reference to identify miRNAs in <i>Helicoverpa armigera</i> and <i>Spodoptera litura</i> using deep sequencing and homology analysis. Because all three species belong to the Lepidoptera, the experiment produced reliable results. Our study identified 97 and 91 conserved miRNAs in <i>H. armigera</i> and <i>S. litura</i>, respectively. Using the genome of <i>B. mori</i> and BAC sequences of <i>H. armigera</i> as references, 1 novel miRNA and 8 novel miRNA candidates were identified in <i>H. armigera</i>, and 4 novel miRNA candidates were identified in <i>S. litura</i>. An evolutionary analysis revealed that most of the identified miRNAs were insect-specific, and more than 20 miRNAs were Lepidoptera-specific. The investigation of the expression patterns of <i>miR-2a</i>, <i>miR-34</i>, <i>miR-2796-3p</i> and <i>miR-11</i> revealed their potential roles in insect development. miRNA target prediction revealed that conserved miRNA target sites exist in various genes in the 3 species. Conserved miRNA target sites for the <i>Hsp90</i> gene among the 3 species were validated in the mammalian 293T cell line using a dual-luciferase reporter assay. Our study provides a new approach with which to identify miRNAs in insects lacking genome information and contributes to the functional analysis of insect miRNAs.</p>http://www.biolsci.org/v09p0001.htm |
| spellingShingle | Xie Ge, Yong Zhang, Jianhao Jiang, Yi Zhong, Xiaonan Yang, Zhiqian Li, Yongping Huang, Anjiang Tan Identification of MicroRNAs in Helicoverpa armigera and Spodoptera litura Based on Deep Sequencing and Homology Analysis International Journal of Biological Sciences |
| title | Identification of MicroRNAs in Helicoverpa armigera and Spodoptera litura Based on Deep Sequencing and Homology Analysis |
| title_full | Identification of MicroRNAs in Helicoverpa armigera and Spodoptera litura Based on Deep Sequencing and Homology Analysis |
| title_fullStr | Identification of MicroRNAs in Helicoverpa armigera and Spodoptera litura Based on Deep Sequencing and Homology Analysis |
| title_full_unstemmed | Identification of MicroRNAs in Helicoverpa armigera and Spodoptera litura Based on Deep Sequencing and Homology Analysis |
| title_short | Identification of MicroRNAs in Helicoverpa armigera and Spodoptera litura Based on Deep Sequencing and Homology Analysis |
| title_sort | identification of micrornas in helicoverpa armigera and spodoptera litura based on deep sequencing and homology analysis |
| url | http://www.biolsci.org/v09p0001.htm |
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