Isolation, Identification, Anti-Inflammatory, and In Silico Analysis of New Lignans from the Resin of <i>Ferula sinkiangensis</i>
<i>Ferula sinkiangensis</i> K. M. Shen (Apiaceae) is distributed in arid desert areas of Xinjiang, and its resin is a traditional Chinese medicine to treat gastrointestinal digestive diseases. To explore bioactive components from <i>F. sinkiangensis</i>, three new lignans and...
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MDPI AG
2023-09-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/10/1351 |
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| author | Junchi Wang Qi Zheng Minghui Shi Huaxiang Wang Congzhao Fan Guoping Wang Yaqin Zhao Jianyong Si |
| author_facet | Junchi Wang Qi Zheng Minghui Shi Huaxiang Wang Congzhao Fan Guoping Wang Yaqin Zhao Jianyong Si |
| author_sort | Junchi Wang |
| collection | DOAJ |
| description | <i>Ferula sinkiangensis</i> K. M. Shen (Apiaceae) is distributed in arid desert areas of Xinjiang, and its resin is a traditional Chinese medicine to treat gastrointestinal digestive diseases. To explore bioactive components from <i>F. sinkiangensis</i>, three new lignans and thirteen known components were isolated. The structural elucidation of the components was established utilizing spectroscopic analyses together with ECD calculations. Griess reaction results indicated new compounds <b>1</b> and <b>2</b> significantly decreased NO production in LPS-stimulated RAW 264.7 macrophages, and ELISA results indicated that they effectively attenuated LPS-induced inflammation by inhibiting TNF-α, IL-1β, and IL-6 expressions. The in silico approach confirmed that compound <b>1</b> docked into the receptors with strong binding energies of −5.84~−10.79 kcal/mol. In addition, compound <b>6</b> inhibited the proliferation of AGS gastric cancer cells with IC<sub>50</sub> values of 15.2 μM by suppressing the cell migration and invasion. This study disclosed that <i>F. sinkiangensis</i> might be a promising potential resource for bioactive components. |
| first_indexed | 2024-03-10T20:58:57Z |
| format | Article |
| id | doaj.art-ac8e09fc10174402b23b82dbd69db75f |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T20:58:57Z |
| publishDate | 2023-09-01 |
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| series | Pharmaceuticals |
| spelling | doaj.art-ac8e09fc10174402b23b82dbd69db75f2023-11-19T17:41:14ZengMDPI AGPharmaceuticals1424-82472023-09-011610135110.3390/ph16101351Isolation, Identification, Anti-Inflammatory, and In Silico Analysis of New Lignans from the Resin of <i>Ferula sinkiangensis</i>Junchi Wang0Qi Zheng1Minghui Shi2Huaxiang Wang3Congzhao Fan4Guoping Wang5Yaqin Zhao6Jianyong Si7The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaThe Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaXinjiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, ChinaThe Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaXinjiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, ChinaXinjiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, ChinaXinjiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, ChinaThe Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China<i>Ferula sinkiangensis</i> K. M. Shen (Apiaceae) is distributed in arid desert areas of Xinjiang, and its resin is a traditional Chinese medicine to treat gastrointestinal digestive diseases. To explore bioactive components from <i>F. sinkiangensis</i>, three new lignans and thirteen known components were isolated. The structural elucidation of the components was established utilizing spectroscopic analyses together with ECD calculations. Griess reaction results indicated new compounds <b>1</b> and <b>2</b> significantly decreased NO production in LPS-stimulated RAW 264.7 macrophages, and ELISA results indicated that they effectively attenuated LPS-induced inflammation by inhibiting TNF-α, IL-1β, and IL-6 expressions. The in silico approach confirmed that compound <b>1</b> docked into the receptors with strong binding energies of −5.84~−10.79 kcal/mol. In addition, compound <b>6</b> inhibited the proliferation of AGS gastric cancer cells with IC<sub>50</sub> values of 15.2 μM by suppressing the cell migration and invasion. This study disclosed that <i>F. sinkiangensis</i> might be a promising potential resource for bioactive components.https://www.mdpi.com/1424-8247/16/10/1351<i>Ferula sinkiangensis</i>lignanstructural elucidationECDanti-inflammatorymolecular docking |
| spellingShingle | Junchi Wang Qi Zheng Minghui Shi Huaxiang Wang Congzhao Fan Guoping Wang Yaqin Zhao Jianyong Si Isolation, Identification, Anti-Inflammatory, and In Silico Analysis of New Lignans from the Resin of <i>Ferula sinkiangensis</i> Pharmaceuticals <i>Ferula sinkiangensis</i> lignan structural elucidation ECD anti-inflammatory molecular docking |
| title | Isolation, Identification, Anti-Inflammatory, and In Silico Analysis of New Lignans from the Resin of <i>Ferula sinkiangensis</i> |
| title_full | Isolation, Identification, Anti-Inflammatory, and In Silico Analysis of New Lignans from the Resin of <i>Ferula sinkiangensis</i> |
| title_fullStr | Isolation, Identification, Anti-Inflammatory, and In Silico Analysis of New Lignans from the Resin of <i>Ferula sinkiangensis</i> |
| title_full_unstemmed | Isolation, Identification, Anti-Inflammatory, and In Silico Analysis of New Lignans from the Resin of <i>Ferula sinkiangensis</i> |
| title_short | Isolation, Identification, Anti-Inflammatory, and In Silico Analysis of New Lignans from the Resin of <i>Ferula sinkiangensis</i> |
| title_sort | isolation identification anti inflammatory and in silico analysis of new lignans from the resin of i ferula sinkiangensis i |
| topic | <i>Ferula sinkiangensis</i> lignan structural elucidation ECD anti-inflammatory molecular docking |
| url | https://www.mdpi.com/1424-8247/16/10/1351 |
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