Effects of sterilization on nanogel-based universal coatings: An essential step for clinical translation
Implant associated infections are a serious threat to the well-being of patients, which can be mitigated by taking effective disinfection/sterilization (D/S) methods into account. Nanogels (nGel) are stimuli sensitive polymeric hydrogel particles, which have provided numerous innovative applications...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127524000613 |
| _version_ | 1797301609109127168 |
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| author | Devlina Ghosh Brandon W. Peterson Cees de Waal Joop de Vries Hans Kaper Guangyue Zu Max Witjes Patrick van Rijn |
| author_facet | Devlina Ghosh Brandon W. Peterson Cees de Waal Joop de Vries Hans Kaper Guangyue Zu Max Witjes Patrick van Rijn |
| author_sort | Devlina Ghosh |
| collection | DOAJ |
| description | Implant associated infections are a serious threat to the well-being of patients, which can be mitigated by taking effective disinfection/sterilization (D/S) methods into account. Nanogels (nGel) are stimuli sensitive polymeric hydrogel particles, which have provided numerous innovative applications in the biomedical field to enhance antifouling, antibacterial properties, or drug delivery, or they can be employed as imaging modalities or can be applied as a coating on biomaterials (implants). Prior to translating their application towards clinical use, nGel-based coated implant materials must undergo an intermediary, pre-requisite process of cleaning, disinfection, and sterilization, in sequence. The interplay among the three crucial pillars- the implant material, the nGel coating (with specific function), and the applied D/S processes influence the fate (success or failure) of medical implant in the host body. In this study, we investigated a previously developed NIPAM-co-APMA core shell nGel coating on various clinically-relevant polymeric and inorganic implant materials and tested them on a diverse range of D/S techniques to assess the retention of the coating quality and antifouling function. The stability and integrity of the nGel coating was analyzed by performing Atomic Force Microscopy and the retention of the antifouling function of the nGel-coating after sterilization was studied by Colony forming units against S. aureus RN4220. Among all the materials that were coated, polymeric materials- polypropylene and polyetheretherketone exhibited exceptional coating stability, post-sterilization while also demonstrating a considerable reduction in bacterial attachment with respect to their ‘uncoated, sterilized’ and ‘coated, non-sterilized’ controls. Although often overlooked, sterilization is an indispensable part of clinical translation, therefore research in this domain is of utmost importance when considering clinical translatability. |
| first_indexed | 2024-03-07T23:25:01Z |
| format | Article |
| id | doaj.art-ac91258581454aeaad87a01dd027628a |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-03-07T23:25:01Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-ac91258581454aeaad87a01dd027628a2024-02-21T05:24:05ZengElsevierMaterials & Design0264-12752024-02-01238112689Effects of sterilization on nanogel-based universal coatings: An essential step for clinical translationDevlina Ghosh0Brandon W. Peterson1Cees de Waal2Joop de Vries3Hans Kaper4Guangyue Zu5Max Witjes6Patrick van Rijn7University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, Antonius Deusinglaan 1, 9713 AV Groningen, the NetherlandsUniversity of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, Antonius Deusinglaan 1, 9713 AV Groningen, the NetherlandsSteriNoord, Jeverweg 3e, 9723 JE Groningen, the NetherlandsUniversity of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, Antonius Deusinglaan 1, 9713 AV Groningen, the NetherlandsUniversity of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, Antonius Deusinglaan 1, 9713 AV Groningen, the NetherlandsUniversity of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, Antonius Deusinglaan 1, 9713 AV Groningen, the NetherlandsUniversity of Groningen, University Medical Center Groningen, Department of Oral and Maxillofacial Surgery, Hanzeplein 1 BB70, 9713 GX Groningen, the NetherlandsUniversity of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands; Corresponding author.Implant associated infections are a serious threat to the well-being of patients, which can be mitigated by taking effective disinfection/sterilization (D/S) methods into account. Nanogels (nGel) are stimuli sensitive polymeric hydrogel particles, which have provided numerous innovative applications in the biomedical field to enhance antifouling, antibacterial properties, or drug delivery, or they can be employed as imaging modalities or can be applied as a coating on biomaterials (implants). Prior to translating their application towards clinical use, nGel-based coated implant materials must undergo an intermediary, pre-requisite process of cleaning, disinfection, and sterilization, in sequence. The interplay among the three crucial pillars- the implant material, the nGel coating (with specific function), and the applied D/S processes influence the fate (success or failure) of medical implant in the host body. In this study, we investigated a previously developed NIPAM-co-APMA core shell nGel coating on various clinically-relevant polymeric and inorganic implant materials and tested them on a diverse range of D/S techniques to assess the retention of the coating quality and antifouling function. The stability and integrity of the nGel coating was analyzed by performing Atomic Force Microscopy and the retention of the antifouling function of the nGel-coating after sterilization was studied by Colony forming units against S. aureus RN4220. Among all the materials that were coated, polymeric materials- polypropylene and polyetheretherketone exhibited exceptional coating stability, post-sterilization while also demonstrating a considerable reduction in bacterial attachment with respect to their ‘uncoated, sterilized’ and ‘coated, non-sterilized’ controls. Although often overlooked, sterilization is an indispensable part of clinical translation, therefore research in this domain is of utmost importance when considering clinical translatability.http://www.sciencedirect.com/science/article/pii/S0264127524000613NanogelSterilizationCoatingAntifoulingBacterial adhesion |
| spellingShingle | Devlina Ghosh Brandon W. Peterson Cees de Waal Joop de Vries Hans Kaper Guangyue Zu Max Witjes Patrick van Rijn Effects of sterilization on nanogel-based universal coatings: An essential step for clinical translation Materials & Design Nanogel Sterilization Coating Antifouling Bacterial adhesion |
| title | Effects of sterilization on nanogel-based universal coatings: An essential step for clinical translation |
| title_full | Effects of sterilization on nanogel-based universal coatings: An essential step for clinical translation |
| title_fullStr | Effects of sterilization on nanogel-based universal coatings: An essential step for clinical translation |
| title_full_unstemmed | Effects of sterilization on nanogel-based universal coatings: An essential step for clinical translation |
| title_short | Effects of sterilization on nanogel-based universal coatings: An essential step for clinical translation |
| title_sort | effects of sterilization on nanogel based universal coatings an essential step for clinical translation |
| topic | Nanogel Sterilization Coating Antifouling Bacterial adhesion |
| url | http://www.sciencedirect.com/science/article/pii/S0264127524000613 |
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