Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula

Legumes are one of the world's leading sources of nutrition, providing sustainable agriculture. They require minimal amounts of soil improvers (fertilizers) due to the ability to absorb nitrogen through symbiotic interactions with nitrogen-fixing soil microorganisms. The trend for human populat...

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Main Authors: Mariana Radkova, Miglena Revalska, Daniela Kertikova, Anelia Iantcheva
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Online Access:http://dx.doi.org/10.1080/13102818.2019.1568914
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author Mariana Radkova
Miglena Revalska
Daniela Kertikova
Anelia Iantcheva
author_facet Mariana Radkova
Miglena Revalska
Daniela Kertikova
Anelia Iantcheva
author_sort Mariana Radkova
collection DOAJ
description Legumes are one of the world's leading sources of nutrition, providing sustainable agriculture. They require minimal amounts of soil improvers (fertilizers) due to the ability to absorb nitrogen through symbiotic interactions with nitrogen-fixing soil microorganisms. The trend for human population growth requires an adequate growth in crop production. This directly depends on the seed size and number as well as on the leaf biomass. In turn, seed size has been the subject of selection programmers for all crops. In this study, we explored the relation between the function of the gene coding Zinc finger CCHC-type protein and flower morphology and seed size in the model legume species Medicago truncatula. M. truncatula lines with modified level of Zinc finger CCHC transcript and transcriptional reporters were developed and analysed by real-time polymerase chain reaction and expression of the GUS (β-glucuronidase) and green fluorescent protein (GFP) reporter genes. A tissue-specific GFP signal was detected in the anthers from overexpessing M. truncatula lines. The M. truncatula lines with knockdown expression showed direct relation between low transcription level of Mt-Zn-CCHC gene and strongly reduced seed size accompanied with short stems length and internodes.
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spelling doaj.art-ac92ad6bbfc540179ea1625a60fa02e82022-12-22T02:23:36ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-0133127828510.1080/13102818.2019.15689141568914Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatulaMariana Radkova0Miglena Revalska1Daniela Kertikova2Anelia Iantcheva3AgroBioInstitute, Agricultural AcademyAgroBioInstitute, Agricultural AcademyInstitute of Forage Crops, Agricultural AcademyAgroBioInstitute, Agricultural AcademyLegumes are one of the world's leading sources of nutrition, providing sustainable agriculture. They require minimal amounts of soil improvers (fertilizers) due to the ability to absorb nitrogen through symbiotic interactions with nitrogen-fixing soil microorganisms. The trend for human population growth requires an adequate growth in crop production. This directly depends on the seed size and number as well as on the leaf biomass. In turn, seed size has been the subject of selection programmers for all crops. In this study, we explored the relation between the function of the gene coding Zinc finger CCHC-type protein and flower morphology and seed size in the model legume species Medicago truncatula. M. truncatula lines with modified level of Zinc finger CCHC transcript and transcriptional reporters were developed and analysed by real-time polymerase chain reaction and expression of the GUS (β-glucuronidase) and green fluorescent protein (GFP) reporter genes. A tissue-specific GFP signal was detected in the anthers from overexpessing M. truncatula lines. The M. truncatula lines with knockdown expression showed direct relation between low transcription level of Mt-Zn-CCHC gene and strongly reduced seed size accompanied with short stems length and internodes.http://dx.doi.org/10.1080/13102818.2019.1568914model legume medicago truncatulagene coding zinc finger cchc-type proteintranscription factorseed size
spellingShingle Mariana Radkova
Miglena Revalska
Daniela Kertikova
Anelia Iantcheva
Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula
Biotechnology & Biotechnological Equipment
model legume medicago truncatula
gene coding zinc finger cchc-type protein
transcription factor
seed size
title Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula
title_full Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula
title_fullStr Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula
title_full_unstemmed Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula
title_short Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula
title_sort zinc finger cchc type protein related with seed size in model legume species medicago truncatula
topic model legume medicago truncatula
gene coding zinc finger cchc-type protein
transcription factor
seed size
url http://dx.doi.org/10.1080/13102818.2019.1568914
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AT danielakertikova zincfingercchctypeproteinrelatedwithseedsizeinmodellegumespeciesmedicagotruncatula
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