Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula
Legumes are one of the world's leading sources of nutrition, providing sustainable agriculture. They require minimal amounts of soil improvers (fertilizers) due to the ability to absorb nitrogen through symbiotic interactions with nitrogen-fixing soil microorganisms. The trend for human populat...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2019-01-01
|
Series: | Biotechnology & Biotechnological Equipment |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/13102818.2019.1568914 |
_version_ | 1817985939174064128 |
---|---|
author | Mariana Radkova Miglena Revalska Daniela Kertikova Anelia Iantcheva |
author_facet | Mariana Radkova Miglena Revalska Daniela Kertikova Anelia Iantcheva |
author_sort | Mariana Radkova |
collection | DOAJ |
description | Legumes are one of the world's leading sources of nutrition, providing sustainable agriculture. They require minimal amounts of soil improvers (fertilizers) due to the ability to absorb nitrogen through symbiotic interactions with nitrogen-fixing soil microorganisms. The trend for human population growth requires an adequate growth in crop production. This directly depends on the seed size and number as well as on the leaf biomass. In turn, seed size has been the subject of selection programmers for all crops. In this study, we explored the relation between the function of the gene coding Zinc finger CCHC-type protein and flower morphology and seed size in the model legume species Medicago truncatula. M. truncatula lines with modified level of Zinc finger CCHC transcript and transcriptional reporters were developed and analysed by real-time polymerase chain reaction and expression of the GUS (β-glucuronidase) and green fluorescent protein (GFP) reporter genes. A tissue-specific GFP signal was detected in the anthers from overexpessing M. truncatula lines. The M. truncatula lines with knockdown expression showed direct relation between low transcription level of Mt-Zn-CCHC gene and strongly reduced seed size accompanied with short stems length and internodes. |
first_indexed | 2024-04-14T00:04:10Z |
format | Article |
id | doaj.art-ac92ad6bbfc540179ea1625a60fa02e8 |
institution | Directory Open Access Journal |
issn | 1310-2818 1314-3530 |
language | English |
last_indexed | 2024-04-14T00:04:10Z |
publishDate | 2019-01-01 |
publisher | Taylor & Francis Group |
record_format | Article |
series | Biotechnology & Biotechnological Equipment |
spelling | doaj.art-ac92ad6bbfc540179ea1625a60fa02e82022-12-22T02:23:36ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-0133127828510.1080/13102818.2019.15689141568914Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatulaMariana Radkova0Miglena Revalska1Daniela Kertikova2Anelia Iantcheva3AgroBioInstitute, Agricultural AcademyAgroBioInstitute, Agricultural AcademyInstitute of Forage Crops, Agricultural AcademyAgroBioInstitute, Agricultural AcademyLegumes are one of the world's leading sources of nutrition, providing sustainable agriculture. They require minimal amounts of soil improvers (fertilizers) due to the ability to absorb nitrogen through symbiotic interactions with nitrogen-fixing soil microorganisms. The trend for human population growth requires an adequate growth in crop production. This directly depends on the seed size and number as well as on the leaf biomass. In turn, seed size has been the subject of selection programmers for all crops. In this study, we explored the relation between the function of the gene coding Zinc finger CCHC-type protein and flower morphology and seed size in the model legume species Medicago truncatula. M. truncatula lines with modified level of Zinc finger CCHC transcript and transcriptional reporters were developed and analysed by real-time polymerase chain reaction and expression of the GUS (β-glucuronidase) and green fluorescent protein (GFP) reporter genes. A tissue-specific GFP signal was detected in the anthers from overexpessing M. truncatula lines. The M. truncatula lines with knockdown expression showed direct relation between low transcription level of Mt-Zn-CCHC gene and strongly reduced seed size accompanied with short stems length and internodes.http://dx.doi.org/10.1080/13102818.2019.1568914model legume medicago truncatulagene coding zinc finger cchc-type proteintranscription factorseed size |
spellingShingle | Mariana Radkova Miglena Revalska Daniela Kertikova Anelia Iantcheva Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula Biotechnology & Biotechnological Equipment model legume medicago truncatula gene coding zinc finger cchc-type protein transcription factor seed size |
title | Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula |
title_full | Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula |
title_fullStr | Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula |
title_full_unstemmed | Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula |
title_short | Zinc finger CCHC-type protein related with seed size in model legume species Medicago truncatula |
title_sort | zinc finger cchc type protein related with seed size in model legume species medicago truncatula |
topic | model legume medicago truncatula gene coding zinc finger cchc-type protein transcription factor seed size |
url | http://dx.doi.org/10.1080/13102818.2019.1568914 |
work_keys_str_mv | AT marianaradkova zincfingercchctypeproteinrelatedwithseedsizeinmodellegumespeciesmedicagotruncatula AT miglenarevalska zincfingercchctypeproteinrelatedwithseedsizeinmodellegumespeciesmedicagotruncatula AT danielakertikova zincfingercchctypeproteinrelatedwithseedsizeinmodellegumespeciesmedicagotruncatula AT aneliaiantcheva zincfingercchctypeproteinrelatedwithseedsizeinmodellegumespeciesmedicagotruncatula |