Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit
<i>Toxoplasma gondii</i> is a protozoan parasite that causes toxoplasmosis in warm-blooded vertebrates, globally. The main aims of this study were to assess the seropositivity to toxoplasmosis of an exotic breed of cattle (<i>n</i> = 400) from different farms using the Latex...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/16/2067 |
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| author | Sarfraz-ur-Rahman Haroon Akbar Muhammad Zubair Shabbir Ubaid Ullah Muhammad Imran Rashid |
| author_facet | Sarfraz-ur-Rahman Haroon Akbar Muhammad Zubair Shabbir Ubaid Ullah Muhammad Imran Rashid |
| author_sort | Sarfraz-ur-Rahman |
| collection | DOAJ |
| description | <i>Toxoplasma gondii</i> is a protozoan parasite that causes toxoplasmosis in warm-blooded vertebrates, globally. The main aims of this study were to assess the seropositivity to toxoplasmosis of an exotic breed of cattle (<i>n</i> = 400) from different farms using the Latex Agglutination Test and validate Cattle Toxo IgG ELISA kit. Of a total of 400 cattle sera that were evaluated by LAT, 143 (35.75%) were found positive. Based on these data, 90 samples (<i>n</i> = 60 seronegative by LAT; <i>n</i> = 30 seropositive by LAT) were elected for screening through a commercially available ELISA kit. The same 90 samples were screened through a Cattle Toxo IgG ELISA kit for validation purposes. Of 90 samples, 40 were seropositive in the Cattle Toxo IgG ELISA kit (100% sensitivity), and 38 were seropositive in a commercially available ELISA kit. All 50 samples in the Cattle Toxo IgG ELISA kit (96.15% specificity) were also seronegative in the commercially available ELISA kit. Hence, the sensitivity and specificity of the Cattle Toxo IgG ELISA kit came out to be 100% and 96.15%, and in LAT, it was found as 26.31% and 61.53%, respectively. Therefore, the Cattle Toxo IgG ELISA kit is a highly reliable serodiagnostic tool to diagnose bovine toxoplasmosis. |
| first_indexed | 2024-03-09T12:00:55Z |
| format | Article |
| id | doaj.art-ac937925ed6a414a8f0517c3440014a7 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T12:00:55Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ac937925ed6a414a8f0517c3440014a72023-11-30T23:03:51ZengMDPI AGAnimals2076-26152022-08-011216206710.3390/ani12162067Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA KitSarfraz-ur-Rahman0Haroon Akbar1Muhammad Zubair Shabbir2Ubaid Ullah3Muhammad Imran Rashid4Department of Parasitology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Parasitology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanInstitute of Microbiology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Parasitology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Parasitology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan<i>Toxoplasma gondii</i> is a protozoan parasite that causes toxoplasmosis in warm-blooded vertebrates, globally. The main aims of this study were to assess the seropositivity to toxoplasmosis of an exotic breed of cattle (<i>n</i> = 400) from different farms using the Latex Agglutination Test and validate Cattle Toxo IgG ELISA kit. Of a total of 400 cattle sera that were evaluated by LAT, 143 (35.75%) were found positive. Based on these data, 90 samples (<i>n</i> = 60 seronegative by LAT; <i>n</i> = 30 seropositive by LAT) were elected for screening through a commercially available ELISA kit. The same 90 samples were screened through a Cattle Toxo IgG ELISA kit for validation purposes. Of 90 samples, 40 were seropositive in the Cattle Toxo IgG ELISA kit (100% sensitivity), and 38 were seropositive in a commercially available ELISA kit. All 50 samples in the Cattle Toxo IgG ELISA kit (96.15% specificity) were also seronegative in the commercially available ELISA kit. Hence, the sensitivity and specificity of the Cattle Toxo IgG ELISA kit came out to be 100% and 96.15%, and in LAT, it was found as 26.31% and 61.53%, respectively. Therefore, the Cattle Toxo IgG ELISA kit is a highly reliable serodiagnostic tool to diagnose bovine toxoplasmosis.https://www.mdpi.com/2076-2615/12/16/2067cattle toxoplasmosisIgG ELISALATseropositiveseronegativesensitivity |
| spellingShingle | Sarfraz-ur-Rahman Haroon Akbar Muhammad Zubair Shabbir Ubaid Ullah Muhammad Imran Rashid Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit Animals cattle toxoplasmosis IgG ELISA LAT seropositive seronegative sensitivity |
| title | Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit |
| title_full | Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit |
| title_fullStr | Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit |
| title_full_unstemmed | Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit |
| title_short | Serological Investigation of Bovine Toxoplasmosis Using Commercial and Indigenous ELISA Kits While Validating Cattle Toxo IgG ELISA Kit |
| title_sort | serological investigation of bovine toxoplasmosis using commercial and indigenous elisa kits while validating cattle toxo igg elisa kit |
| topic | cattle toxoplasmosis IgG ELISA LAT seropositive seronegative sensitivity |
| url | https://www.mdpi.com/2076-2615/12/16/2067 |
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