Transstadial Transmission of Francisella tularensis holarctica in Mosquitoes, Sweden
In Sweden, human cases of tularemia caused by Francisella tularensis holarctica are assumed to be transmitted by mosquitoes, but how mosquito vectors acquire and transmit the bacterium is not clear. To determine how transmission of this bacterium occurs, mosquito larvae were collected in an area whe...
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2011-05-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/17/5/10-0426_article |
| _version_ | 1819153302254780416 |
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| author | Jan O. Lundström Ann-Christin Andersson Stina Bäckman Martina L. Schäfer Mats Forsman Johanna Thelaus |
| author_facet | Jan O. Lundström Ann-Christin Andersson Stina Bäckman Martina L. Schäfer Mats Forsman Johanna Thelaus |
| author_sort | Jan O. Lundström |
| collection | DOAJ |
| description | In Sweden, human cases of tularemia caused by Francisella tularensis holarctica are assumed to be transmitted by mosquitoes, but how mosquito vectors acquire and transmit the bacterium is not clear. To determine how transmission of this bacterium occurs, mosquito larvae were collected in an area where tularemia is endemic, brought to the laboratory, and reared to adults in their original pond water. Screening of adult mosquitoes by real-time PCR demonstrated F. tularensis lpnA sequences in 14 of the 48 mosquito pools tested; lpnA sequences were demonstrated in 6 of 9 identified mosquito species. Further analysis confirmed the presence of F. tularensis holarctica–specific 30-bp deletion region sequences (FtM19inDel) in water from breeding containers and in 3 mosquito species (Aedes sticticus, Ae. vexans, and Ae. punctor) known to take blood from humans. Our results suggest that the mosquitoes that transmit F. tularensis holarctica during tularemia outbreaks acquire the bacterium already as larvae. |
| first_indexed | 2024-12-22T15:03:01Z |
| format | Article |
| id | doaj.art-ac9b3ddc78c74403b555549753d414d6 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-22T15:03:01Z |
| publishDate | 2011-05-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-ac9b3ddc78c74403b555549753d414d62022-12-21T18:22:03ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592011-05-0117579479910.3201/eid1705.100426Transstadial Transmission of Francisella tularensis holarctica in Mosquitoes, SwedenJan O. LundströmAnn-Christin AnderssonStina BäckmanMartina L. SchäferMats ForsmanJohanna ThelausIn Sweden, human cases of tularemia caused by Francisella tularensis holarctica are assumed to be transmitted by mosquitoes, but how mosquito vectors acquire and transmit the bacterium is not clear. To determine how transmission of this bacterium occurs, mosquito larvae were collected in an area where tularemia is endemic, brought to the laboratory, and reared to adults in their original pond water. Screening of adult mosquitoes by real-time PCR demonstrated F. tularensis lpnA sequences in 14 of the 48 mosquito pools tested; lpnA sequences were demonstrated in 6 of 9 identified mosquito species. Further analysis confirmed the presence of F. tularensis holarctica–specific 30-bp deletion region sequences (FtM19inDel) in water from breeding containers and in 3 mosquito species (Aedes sticticus, Ae. vexans, and Ae. punctor) known to take blood from humans. Our results suggest that the mosquitoes that transmit F. tularensis holarctica during tularemia outbreaks acquire the bacterium already as larvae.https://wwwnc.cdc.gov/eid/article/17/5/10-0426_articleMosquitoesvector-borne infectionsculicidaeFrancisella tularensis holarcticabacteriaAedes sticticus |
| spellingShingle | Jan O. Lundström Ann-Christin Andersson Stina Bäckman Martina L. Schäfer Mats Forsman Johanna Thelaus Transstadial Transmission of Francisella tularensis holarctica in Mosquitoes, Sweden Emerging Infectious Diseases Mosquitoes vector-borne infections culicidae Francisella tularensis holarctica bacteria Aedes sticticus |
| title | Transstadial Transmission of Francisella tularensis holarctica in Mosquitoes, Sweden |
| title_full | Transstadial Transmission of Francisella tularensis holarctica in Mosquitoes, Sweden |
| title_fullStr | Transstadial Transmission of Francisella tularensis holarctica in Mosquitoes, Sweden |
| title_full_unstemmed | Transstadial Transmission of Francisella tularensis holarctica in Mosquitoes, Sweden |
| title_short | Transstadial Transmission of Francisella tularensis holarctica in Mosquitoes, Sweden |
| title_sort | transstadial transmission of francisella tularensis holarctica in mosquitoes sweden |
| topic | Mosquitoes vector-borne infections culicidae Francisella tularensis holarctica bacteria Aedes sticticus |
| url | https://wwwnc.cdc.gov/eid/article/17/5/10-0426_article |
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