Significance of N6-methyladenosine RNA methylation regulators in diagnosis and subtype classification of primary Sjögren’s syndrome
The importance of N6-methyladenine (m6A) in mRNA metabolism, physiology, pathology and other life processes is well recognized. However, the exact role of m6A regulators in primary Sjögren's syndrome (PSS) remains unclear. In this study, we used bioinformatics and machine learning random forest...
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Elsevier
2024-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024008910 |
| _version_ | 1797304572342960128 |
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| author | Jiaoyan Li Kaihong Xie Minxian Xu Ye Wang Yinghong Huang Tao Tan Hui Xie |
| author_facet | Jiaoyan Li Kaihong Xie Minxian Xu Ye Wang Yinghong Huang Tao Tan Hui Xie |
| author_sort | Jiaoyan Li |
| collection | DOAJ |
| description | The importance of N6-methyladenine (m6A) in mRNA metabolism, physiology, pathology and other life processes is well recognized. However, the exact role of m6A regulators in primary Sjögren's syndrome (PSS) remains unclear. In this study, we used bioinformatics and machine learning random forest approach to screen eight key m6A regulators from the Gene Expression Omnibus GSE7451, GSE40611 and GSE84844 datasets. An accurate nomogram model for predicting PSS risk was established based on these regulators. And using consensus clustering, patients diagnosed with PSS were classified into two different m6A patterns. We found that patients in group B had higher m6A scores compared to those in group A: furthermore, both groups were closely related to immunity and possibly to other diseases. These results emphasise the important role of m6A regulators in the pathogenesis of PSS. Our study of m6A patterns may inform future immunotherapy strategies for PSS. |
| first_indexed | 2024-03-08T00:12:22Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T00:12:22Z |
| publishDate | 2024-02-01 |
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| spelling | doaj.art-aca0b715ffd1461bbc732f9b730920d32024-02-17T06:38:54ZengElsevierHeliyon2405-84402024-02-01103e24860Significance of N6-methyladenosine RNA methylation regulators in diagnosis and subtype classification of primary Sjögren’s syndromeJiaoyan Li0Kaihong Xie1Minxian Xu2Ye Wang3Yinghong Huang4Tao Tan5Hui Xie6Department of Rheumatology and Clinical Immunology, The First Hospital of Changsha, Changsha, 410005, Hunan Province, PR ChinaDepartment of Oncology, Affiliated Hospital (Clinical College) of Xiangnan University, Chenzhou, 423000, Hunan Province, PR ChinaDepartment of Oncology, Affiliated Hospital (Clinical College) of Xiangnan University, Chenzhou, 423000, Hunan Province, PR ChinaDepartment of Thoracic Surgery, Affiliated Hospital (Clinical College) of Xiangnan University, Chenzhou, 423000, Hunan Province, PR ChinaDepartment of Rheumatology and Clinical Immunology, The First Hospital of Changsha, Changsha, 410005, Hunan Province, PR ChinaFaulty of Applied Sciences, Macao Polytechnic University, Macao, 999078, PR China; Corresponding author.Faulty of Applied Sciences, Macao Polytechnic University, Macao, 999078, PR China; Department of Radiation Oncology, Affiliated Hospital (Clinical College) of Xiangnan University, Chenzhou, 423000, Hunan Province, PR China; Corresponding author. Department of Radiation Oncology, Affiliated Hospital (Clinical College) of Xiangnan University, No. 25, Renmin West Road, Chenzhou, 423000, Hunan Province, PR China.The importance of N6-methyladenine (m6A) in mRNA metabolism, physiology, pathology and other life processes is well recognized. However, the exact role of m6A regulators in primary Sjögren's syndrome (PSS) remains unclear. In this study, we used bioinformatics and machine learning random forest approach to screen eight key m6A regulators from the Gene Expression Omnibus GSE7451, GSE40611 and GSE84844 datasets. An accurate nomogram model for predicting PSS risk was established based on these regulators. And using consensus clustering, patients diagnosed with PSS were classified into two different m6A patterns. We found that patients in group B had higher m6A scores compared to those in group A: furthermore, both groups were closely related to immunity and possibly to other diseases. These results emphasise the important role of m6A regulators in the pathogenesis of PSS. Our study of m6A patterns may inform future immunotherapy strategies for PSS.http://www.sciencedirect.com/science/article/pii/S2405844024008910N6-methyladenosinem6ARNA methylation regulatorsPrimary Sjögren’s syndromePSSGene expression omnibus database |
| spellingShingle | Jiaoyan Li Kaihong Xie Minxian Xu Ye Wang Yinghong Huang Tao Tan Hui Xie Significance of N6-methyladenosine RNA methylation regulators in diagnosis and subtype classification of primary Sjögren’s syndrome Heliyon N6-methyladenosine m6A RNA methylation regulators Primary Sjögren’s syndrome PSS Gene expression omnibus database |
| title | Significance of N6-methyladenosine RNA methylation regulators in diagnosis and subtype classification of primary Sjögren’s syndrome |
| title_full | Significance of N6-methyladenosine RNA methylation regulators in diagnosis and subtype classification of primary Sjögren’s syndrome |
| title_fullStr | Significance of N6-methyladenosine RNA methylation regulators in diagnosis and subtype classification of primary Sjögren’s syndrome |
| title_full_unstemmed | Significance of N6-methyladenosine RNA methylation regulators in diagnosis and subtype classification of primary Sjögren’s syndrome |
| title_short | Significance of N6-methyladenosine RNA methylation regulators in diagnosis and subtype classification of primary Sjögren’s syndrome |
| title_sort | significance of n6 methyladenosine rna methylation regulators in diagnosis and subtype classification of primary sjogren s syndrome |
| topic | N6-methyladenosine m6A RNA methylation regulators Primary Sjögren’s syndrome PSS Gene expression omnibus database |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024008910 |
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