Adaptation of the carbamoyl-phosphate synthetase enzyme in an extremophile fish
Tetrapods and fish have adapted distinct carbamoyl-phosphate synthase (CPS) enzymes to initiate the ornithine urea cycle during the detoxification of nitrogenous wastes. We report evidence that in the ureotelic subgenus of extremophile fish Oreochromis Alcolapia, CPS III has undergone convergent evo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2020-10-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.201200 |
| _version_ | 1828812778778918912 |
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| author | Lewis J. White Gemma Sutton Asilatu Shechonge Julia J. Day Kanchon K. Dasmahapatra Mary E. Pownall |
| author_facet | Lewis J. White Gemma Sutton Asilatu Shechonge Julia J. Day Kanchon K. Dasmahapatra Mary E. Pownall |
| author_sort | Lewis J. White |
| collection | DOAJ |
| description | Tetrapods and fish have adapted distinct carbamoyl-phosphate synthase (CPS) enzymes to initiate the ornithine urea cycle during the detoxification of nitrogenous wastes. We report evidence that in the ureotelic subgenus of extremophile fish Oreochromis Alcolapia, CPS III has undergone convergent evolution and adapted its substrate affinity to ammonia, which is typical of terrestrial vertebrate CPS I. Unusually, unlike in other vertebrates, the expression of CPS III in Alcolapia is localized to the skeletal muscle and is activated in the myogenic lineage during early embryonic development with expression remaining in mature fish. We propose that adaptation in Alcolapia included both convergent evolution of CPS function to that of terrestrial vertebrates, as well as changes in development mechanisms redirecting CPS III gene expression to the skeletal muscle. |
| first_indexed | 2024-12-12T09:49:22Z |
| format | Article |
| id | doaj.art-aca69784068e419d9b7a9a0b85b9aaee |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-12T09:49:22Z |
| publishDate | 2020-10-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-aca69784068e419d9b7a9a0b85b9aaee2022-12-22T00:28:19ZengThe Royal SocietyRoyal Society Open Science2054-57032020-10-0171010.1098/rsos.201200201200Adaptation of the carbamoyl-phosphate synthetase enzyme in an extremophile fishLewis J. WhiteGemma SuttonAsilatu ShechongeJulia J. DayKanchon K. DasmahapatraMary E. PownallTetrapods and fish have adapted distinct carbamoyl-phosphate synthase (CPS) enzymes to initiate the ornithine urea cycle during the detoxification of nitrogenous wastes. We report evidence that in the ureotelic subgenus of extremophile fish Oreochromis Alcolapia, CPS III has undergone convergent evolution and adapted its substrate affinity to ammonia, which is typical of terrestrial vertebrate CPS I. Unusually, unlike in other vertebrates, the expression of CPS III in Alcolapia is localized to the skeletal muscle and is activated in the myogenic lineage during early embryonic development with expression remaining in mature fish. We propose that adaptation in Alcolapia included both convergent evolution of CPS function to that of terrestrial vertebrates, as well as changes in development mechanisms redirecting CPS III gene expression to the skeletal muscle.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.201200ureacarbamoyl-phosphate synthetasealcolapiaextremophilefish |
| spellingShingle | Lewis J. White Gemma Sutton Asilatu Shechonge Julia J. Day Kanchon K. Dasmahapatra Mary E. Pownall Adaptation of the carbamoyl-phosphate synthetase enzyme in an extremophile fish Royal Society Open Science urea carbamoyl-phosphate synthetase alcolapia extremophile fish |
| title | Adaptation of the carbamoyl-phosphate synthetase enzyme in an extremophile fish |
| title_full | Adaptation of the carbamoyl-phosphate synthetase enzyme in an extremophile fish |
| title_fullStr | Adaptation of the carbamoyl-phosphate synthetase enzyme in an extremophile fish |
| title_full_unstemmed | Adaptation of the carbamoyl-phosphate synthetase enzyme in an extremophile fish |
| title_short | Adaptation of the carbamoyl-phosphate synthetase enzyme in an extremophile fish |
| title_sort | adaptation of the carbamoyl phosphate synthetase enzyme in an extremophile fish |
| topic | urea carbamoyl-phosphate synthetase alcolapia extremophile fish |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.201200 |
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