M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis
BackgroundEndometriosis is a common nonmalignant gynecological disorder that affects 10–15% women of reproductive age and causes several symptoms that result in decreased quality of life and a huge social burden. In recent decades, extracellular vesicles (EVs) have gained attention as a potential th...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.707784/full |
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| author | Qiuju Li Ming Yuan Xue Jiao Yufei Huang Jing Li Dong Li Miaomiao Ji Guoyun Wang |
| author_facet | Qiuju Li Ming Yuan Xue Jiao Yufei Huang Jing Li Dong Li Miaomiao Ji Guoyun Wang |
| author_sort | Qiuju Li |
| collection | DOAJ |
| description | BackgroundEndometriosis is a common nonmalignant gynecological disorder that affects 10–15% women of reproductive age and causes several symptoms that result in decreased quality of life and a huge social burden. In recent decades, extracellular vesicles (EVs) have gained attention as a potential therapeutic tool; however, the therapeutic effects of EVs against endometriosis have not been reported. Accordingly, in this study, we investigated the feasibility of nanovesicles (NVs) derived from M1 macrophages (M1NVs) in treating endometriosis.MethodsM1NVs were prepared by serial extrusion. Co-culture assays were performed to investigate changes in tube formation and migration/invasion of eutopic endometrial stroma cells (ESCs) obtained from patients with endometriosis (EM-ESCs). A mouse model of endometriosis was established, and mice were treated with phosphate-buffered saline, M0NVs, or M1NVs to evaluate the efficacy and safety of M1NV for treating endometriosis.ResultsM1NVs directly or indirectly inhibited the migration and invasion of EM-ESCs and reduced tube formation. In the mouse model, M1NVs suppressed the development of endometriosis through reprogramming of M2 macrophages, without causing damage to the organs.ConclusionsM1NVs inhibit the development of endometriosis directly, or through repolarizing macrophages from M2 to M1 phenotype. Hence, administration of M1NVs may represent a novel method for the treatment of endometriosis. |
| first_indexed | 2024-12-19T16:01:14Z |
| format | Article |
| id | doaj.art-acaa59aa8942463cb97b2254e402684e |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-19T16:01:14Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-acaa59aa8942463cb97b2254e402684e2022-12-21T20:14:56ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-07-011210.3389/fimmu.2021.707784707784M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of EndometriosisQiuju Li0Ming Yuan1Xue Jiao2Yufei Huang3Jing Li4Dong Li5Miaomiao Ji6Guoyun Wang7Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, ChinaCryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, ChinaDepartment of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, ChinaBackgroundEndometriosis is a common nonmalignant gynecological disorder that affects 10–15% women of reproductive age and causes several symptoms that result in decreased quality of life and a huge social burden. In recent decades, extracellular vesicles (EVs) have gained attention as a potential therapeutic tool; however, the therapeutic effects of EVs against endometriosis have not been reported. Accordingly, in this study, we investigated the feasibility of nanovesicles (NVs) derived from M1 macrophages (M1NVs) in treating endometriosis.MethodsM1NVs were prepared by serial extrusion. Co-culture assays were performed to investigate changes in tube formation and migration/invasion of eutopic endometrial stroma cells (ESCs) obtained from patients with endometriosis (EM-ESCs). A mouse model of endometriosis was established, and mice were treated with phosphate-buffered saline, M0NVs, or M1NVs to evaluate the efficacy and safety of M1NV for treating endometriosis.ResultsM1NVs directly or indirectly inhibited the migration and invasion of EM-ESCs and reduced tube formation. In the mouse model, M1NVs suppressed the development of endometriosis through reprogramming of M2 macrophages, without causing damage to the organs.ConclusionsM1NVs inhibit the development of endometriosis directly, or through repolarizing macrophages from M2 to M1 phenotype. Hence, administration of M1NVs may represent a novel method for the treatment of endometriosis.https://www.frontiersin.org/articles/10.3389/fimmu.2021.707784/fullendometriosismacrophage polarizationreprogrammingextracellular vesiclestreatment |
| spellingShingle | Qiuju Li Ming Yuan Xue Jiao Yufei Huang Jing Li Dong Li Miaomiao Ji Guoyun Wang M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis Frontiers in Immunology endometriosis macrophage polarization reprogramming extracellular vesicles treatment |
| title | M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis |
| title_full | M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis |
| title_fullStr | M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis |
| title_full_unstemmed | M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis |
| title_short | M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis |
| title_sort | m1 macrophage derived nanovesicles repolarize m2 macrophages for inhibiting the development of endometriosis |
| topic | endometriosis macrophage polarization reprogramming extracellular vesicles treatment |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.707784/full |
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