Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution
Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known abou...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/3/553 |
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| author | Sandra Claveau Émilie Nehlig Sébastien Garcia-Argote Sophie Feuillastre Grégory Pieters Hugues A. Girard Jean-Charles Arnault François Treussart Jean-Rémi Bertrand |
| author_facet | Sandra Claveau Émilie Nehlig Sébastien Garcia-Argote Sophie Feuillastre Grégory Pieters Hugues A. Girard Jean-Charles Arnault François Treussart Jean-Rémi Bertrand |
| author_sort | Sandra Claveau |
| collection | DOAJ |
| description | Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and efficacy of nanodiamonds-based therapy in mice. In this report, we studied the capacity of cationic hydrogenated detonation nanodiamonds to carry active small interfering RNA (siRNA) in a mice model of Ewing sarcoma, a bone cancer of young adults due in the vast majority to the <i>EWS-FLI1</i> junction oncogene. Replacing hydrogen gas by its radioactive analog tritium gas led to the formation of labeled nanodiamonds and allowed us to investigate their distribution throughout mouse organs and their excretion in urine and feces. We also demonstrated that siRNA directed against <i>EWS-FLI1</i> inhibited this oncogene expression in tumor xenografted on mice. This work is a significant step to establish cationic hydrogenated detonation nanodiamond as an effective agent for in vivo delivery of active siRNA. |
| first_indexed | 2024-04-12T07:15:14Z |
| format | Article |
| id | doaj.art-acb07f08dcc64ff596b8d1e412979a25 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-04-12T07:15:14Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-acb07f08dcc64ff596b8d1e412979a252022-12-22T03:42:30ZengMDPI AGNanomaterials2079-49912020-03-0110355310.3390/nano10030553nano10030553Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue DistributionSandra Claveau0Émilie Nehlig1Sébastien Garcia-Argote2Sophie Feuillastre3Grégory Pieters4Hugues A. Girard5Jean-Charles Arnault6François Treussart7Jean-Rémi Bertrand8LuMIn, CNRS, ENS Paris-Saclay, CentraleSupélec, Université Paris-Saclay, 91405 Orsay, FranceSCBM, Institut Joliot, CEA, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceSCBM, Institut Joliot, CEA, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceSCBM, Institut Joliot, CEA, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceSCBM, Institut Joliot, CEA, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceDiamond Sensors Laboratory, Institut LIST, CEA, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceDiamond Sensors Laboratory, Institut LIST, CEA, Université Paris-Saclay, 91191 Gif-sur-Yvette, FranceLuMIn, CNRS, ENS Paris-Saclay, CentraleSupélec, Université Paris-Saclay, 91405 Orsay, FranceVectorologie et Thérapeutiques Anticancéreuses, CNRS, Institut Gustave Roussy, Université Paris-Saclay, 94805 Villejuif, FranceNanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and efficacy of nanodiamonds-based therapy in mice. In this report, we studied the capacity of cationic hydrogenated detonation nanodiamonds to carry active small interfering RNA (siRNA) in a mice model of Ewing sarcoma, a bone cancer of young adults due in the vast majority to the <i>EWS-FLI1</i> junction oncogene. Replacing hydrogen gas by its radioactive analog tritium gas led to the formation of labeled nanodiamonds and allowed us to investigate their distribution throughout mouse organs and their excretion in urine and feces. We also demonstrated that siRNA directed against <i>EWS-FLI1</i> inhibited this oncogene expression in tumor xenografted on mice. This work is a significant step to establish cationic hydrogenated detonation nanodiamond as an effective agent for in vivo delivery of active siRNA.https://www.mdpi.com/2079-4991/10/3/553nanodiamondtritiumbiodistributionewing sarcomadrug deliverysirnananomedicine |
| spellingShingle | Sandra Claveau Émilie Nehlig Sébastien Garcia-Argote Sophie Feuillastre Grégory Pieters Hugues A. Girard Jean-Charles Arnault François Treussart Jean-Rémi Bertrand Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution Nanomaterials nanodiamond tritium biodistribution ewing sarcoma drug delivery sirna nanomedicine |
| title | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
| title_full | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
| title_fullStr | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
| title_full_unstemmed | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
| title_short | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
| title_sort | delivery of sirna to ewing sarcoma tumor xenografted on mice using hydrogenated detonation nanodiamonds treatment efficacy and tissue distribution |
| topic | nanodiamond tritium biodistribution ewing sarcoma drug delivery sirna nanomedicine |
| url | https://www.mdpi.com/2079-4991/10/3/553 |
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