Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia
Despite diagnostic advances in microbiology, the etiology of neutropenic fever remains elusive in most cases. In this study, we evaluated the utility of a metagenomic shotgun sequencing based assay for detection of bacteria and viruses in blood samples of patients with febrile neutropenia. We prospe...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202879/?tool=EBI |
| _version_ | 1811238527678021632 |
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| author | Prakhar Vijayvargiya Adeline Feri Mathilde Mairey Cécile Rouillon Patricio R. Jeraldo Zerelda Esquer Garrigos Matthew J. Thoendel Kerryl E. Greenwood-Quaintance M. Rizwan Sohail Priya Sampathkumar Megan T. Spychalla A. K. Stewart Mrinal M. Patnaik Aaron J. Tande Stéphane Cruveiller Irene Hannet Pascale Beurdeley Robin Patel |
| author_facet | Prakhar Vijayvargiya Adeline Feri Mathilde Mairey Cécile Rouillon Patricio R. Jeraldo Zerelda Esquer Garrigos Matthew J. Thoendel Kerryl E. Greenwood-Quaintance M. Rizwan Sohail Priya Sampathkumar Megan T. Spychalla A. K. Stewart Mrinal M. Patnaik Aaron J. Tande Stéphane Cruveiller Irene Hannet Pascale Beurdeley Robin Patel |
| author_sort | Prakhar Vijayvargiya |
| collection | DOAJ |
| description | Despite diagnostic advances in microbiology, the etiology of neutropenic fever remains elusive in most cases. In this study, we evaluated the utility of a metagenomic shotgun sequencing based assay for detection of bacteria and viruses in blood samples of patients with febrile neutropenia. We prospectively enrolled 20 acute leukemia patients and obtained blood from these patients at three time points: 1) anytime from onset of neutropenia until before development of neutropenic fever, 2) within 24 hours of onset of neutropenic fever, 3) 5–7 days after onset of neutropenic fever. Blood samples underwent sample preparation, sequencing and analysis using the iDTECT® Dx Blood v1® platform (PathoQuest, Paris, France). Clinically relevant viruses or bacteria were detected in three cases each by metagenomic shotgun sequencing and blood cultures, albeit with no concordance between the two. Further optimization of sample preparation methods and sequencing platforms is needed before widespread adoption of this technology into clinical practice. |
| first_indexed | 2024-04-12T12:43:50Z |
| format | Article |
| id | doaj.art-acb1b0a449e04e588d7ad314dd5cd4a7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T12:43:50Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-acb1b0a449e04e588d7ad314dd5cd4a72022-12-22T03:32:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01176Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropeniaPrakhar VijayvargiyaAdeline FeriMathilde MaireyCécile RouillonPatricio R. JeraldoZerelda Esquer GarrigosMatthew J. ThoendelKerryl E. Greenwood-QuaintanceM. Rizwan SohailPriya SampathkumarMegan T. SpychallaA. K. StewartMrinal M. PatnaikAaron J. TandeStéphane CruveillerIrene HannetPascale BeurdeleyRobin PatelDespite diagnostic advances in microbiology, the etiology of neutropenic fever remains elusive in most cases. In this study, we evaluated the utility of a metagenomic shotgun sequencing based assay for detection of bacteria and viruses in blood samples of patients with febrile neutropenia. We prospectively enrolled 20 acute leukemia patients and obtained blood from these patients at three time points: 1) anytime from onset of neutropenia until before development of neutropenic fever, 2) within 24 hours of onset of neutropenic fever, 3) 5–7 days after onset of neutropenic fever. Blood samples underwent sample preparation, sequencing and analysis using the iDTECT® Dx Blood v1® platform (PathoQuest, Paris, France). Clinically relevant viruses or bacteria were detected in three cases each by metagenomic shotgun sequencing and blood cultures, albeit with no concordance between the two. Further optimization of sample preparation methods and sequencing platforms is needed before widespread adoption of this technology into clinical practice.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202879/?tool=EBI |
| spellingShingle | Prakhar Vijayvargiya Adeline Feri Mathilde Mairey Cécile Rouillon Patricio R. Jeraldo Zerelda Esquer Garrigos Matthew J. Thoendel Kerryl E. Greenwood-Quaintance M. Rizwan Sohail Priya Sampathkumar Megan T. Spychalla A. K. Stewart Mrinal M. Patnaik Aaron J. Tande Stéphane Cruveiller Irene Hannet Pascale Beurdeley Robin Patel Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia PLoS ONE |
| title | Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia |
| title_full | Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia |
| title_fullStr | Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia |
| title_full_unstemmed | Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia |
| title_short | Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia |
| title_sort | metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202879/?tool=EBI |
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