Amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical properties
Abstract The prevalence of arrhythmia in patients with hypertension has gradually attracted widespread attention. However, the relationship between hypertension and arrhythmia still lacks more attention. Herein, we explore the biomechanical mechanism of arrhythmia in hypertensive rats and the effect...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-78677-5 |
| _version_ | 1818856141221789696 |
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| author | Yifeng Nie Yin He Dong Han Yuansheng Liu Xiang Li |
| author_facet | Yifeng Nie Yin He Dong Han Yuansheng Liu Xiang Li |
| author_sort | Yifeng Nie |
| collection | DOAJ |
| description | Abstract The prevalence of arrhythmia in patients with hypertension has gradually attracted widespread attention. However, the relationship between hypertension and arrhythmia still lacks more attention. Herein, we explore the biomechanical mechanism of arrhythmia in hypertensive rats and the effect of amiodarone on biomechanical properties. We applied micro-mechanics and amiodarone to stimulate single ventricular myocytes to compare changes of mechanical parameters and the mechanism was investigated in biomechanics. Then we verified the expression changes of genes and long non-coding RNAs (lncRNAs) related to myocardial mechanics to explore the effect of amiodarone on biomechanical properties. The results found that the stiffness of ventricular myocytes and calcium ion levels in hypertensive rats were significantly increased and amiodarone could alleviate the intracellular calcium response and biomechanical stimulation. In addition, experiments showed spontaneously hypertensive rats were more likely to induce arrhythmia and preoperative amiodarone intervention significantly reduced the occurrence of arrhythmias. Meanwhile, high-throughput sequencing showed the genes and lncRNAs related to myocardial mechanics changed significantly in the spontaneously hypertensive rats that amiodarone was injected. These results strengthen the evidence that hypertension rats are prone to arrhythmia with abnormal myocardial biomechanical properties. Amiodarone effectively inhibit arrhythmia by improving the myocardial biomechanical properties and weakening the sensitivity of mechanical stretch stimulation. |
| first_indexed | 2024-12-19T08:19:47Z |
| format | Article |
| id | doaj.art-acb3237cf18e4eddbd993e8ca2f44f50 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T08:19:47Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-acb3237cf18e4eddbd993e8ca2f44f502022-12-21T20:29:26ZengNature PortfolioScientific Reports2045-23222020-12-011011810.1038/s41598-020-78677-5Amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical propertiesYifeng Nie0Yin He1Dong Han2Yuansheng Liu3Xiang Li4Emergency Department, Peking University People’s HospitalEmergency Department, Peking University People’s HospitalCAS Center for Excellence in Nanoscience, National Center for Nanoscience and TechnologyEmergency Department, Peking University People’s HospitalCAS Center for Excellence in Nanoscience, National Center for Nanoscience and TechnologyAbstract The prevalence of arrhythmia in patients with hypertension has gradually attracted widespread attention. However, the relationship between hypertension and arrhythmia still lacks more attention. Herein, we explore the biomechanical mechanism of arrhythmia in hypertensive rats and the effect of amiodarone on biomechanical properties. We applied micro-mechanics and amiodarone to stimulate single ventricular myocytes to compare changes of mechanical parameters and the mechanism was investigated in biomechanics. Then we verified the expression changes of genes and long non-coding RNAs (lncRNAs) related to myocardial mechanics to explore the effect of amiodarone on biomechanical properties. The results found that the stiffness of ventricular myocytes and calcium ion levels in hypertensive rats were significantly increased and amiodarone could alleviate the intracellular calcium response and biomechanical stimulation. In addition, experiments showed spontaneously hypertensive rats were more likely to induce arrhythmia and preoperative amiodarone intervention significantly reduced the occurrence of arrhythmias. Meanwhile, high-throughput sequencing showed the genes and lncRNAs related to myocardial mechanics changed significantly in the spontaneously hypertensive rats that amiodarone was injected. These results strengthen the evidence that hypertension rats are prone to arrhythmia with abnormal myocardial biomechanical properties. Amiodarone effectively inhibit arrhythmia by improving the myocardial biomechanical properties and weakening the sensitivity of mechanical stretch stimulation.https://doi.org/10.1038/s41598-020-78677-5 |
| spellingShingle | Yifeng Nie Yin He Dong Han Yuansheng Liu Xiang Li Amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical properties Scientific Reports |
| title | Amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical properties |
| title_full | Amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical properties |
| title_fullStr | Amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical properties |
| title_full_unstemmed | Amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical properties |
| title_short | Amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical properties |
| title_sort | amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical properties |
| url | https://doi.org/10.1038/s41598-020-78677-5 |
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