A modifier screen in the <it>Drosophila </it>eye reveals that <it>aPKC </it>interacts with <it>Glued </it>during central synapse formation
<p>Abstract</p> <p>Background</p> <p>The <it>Glued </it>gene of <it>Drosophila melanogaster </it>encodes the homologue of the vertebrate p150<sup>Glued </sup>subunit of dynactin. The <it>Glued</it><sup>1 </sup>...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-01-01
|
| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/10/77 |
| _version_ | 1818248049493803008 |
|---|---|
| author | Ma Lisha Johns Louise Allen Marcus |
| author_facet | Ma Lisha Johns Louise Allen Marcus |
| author_sort | Ma Lisha |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The <it>Glued </it>gene of <it>Drosophila melanogaster </it>encodes the homologue of the vertebrate p150<sup>Glued </sup>subunit of dynactin. The <it>Glued</it><sup>1 </sup>mutation compromises the dynein-dynactin retrograde motor complex and causes disruptions to the adult eye and the CNS, including sensory neurons and the formation of the giant fiber system neural circuit.</p> <p>Results</p> <p>We performed a 2-stage genetic screen to identify mutations that modified phenotypes caused by over-expression of a dominant-negative Glued protein. We screened over 34,000 flies and isolated 41 mutations that enhanced or suppressed an eye phenotype. Of these, 12 were assayed for interactions in the giant fiber system by which they altered a giant fiber morphological phenotype and/or altered synaptic function between the giant fiber and the tergotrochanteral muscle motorneuron. Six showed interactions including a new allele of <it>atypical protein kinase C </it>(<it>aPKC</it>). We show that this cell polarity regulator interacts with <it>Glued </it>during central synapse formation. We have mapped the five other interacting mutations to discrete chromosomal regions.</p> <p>Conclusion</p> <p>Our results show that an efficient way to screen for genes involved in central synapse formation is to use a two-step strategy in which a screen for altered eye morphology precedes the analysis of central synaptogenesis. This has highlighted a role for <it>aPKC </it>in the formation of an identified central synapse.</p> |
| first_indexed | 2024-12-12T15:14:25Z |
| format | Article |
| id | doaj.art-acb5450028854214be5f7c9d242a5a0d |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-12-12T15:14:25Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-acb5450028854214be5f7c9d242a5a0d2022-12-22T00:20:31ZengBMCBMC Genetics1471-21562009-01-0110177A modifier screen in the <it>Drosophila </it>eye reveals that <it>aPKC </it>interacts with <it>Glued </it>during central synapse formationMa LishaJohns LouiseAllen Marcus<p>Abstract</p> <p>Background</p> <p>The <it>Glued </it>gene of <it>Drosophila melanogaster </it>encodes the homologue of the vertebrate p150<sup>Glued </sup>subunit of dynactin. The <it>Glued</it><sup>1 </sup>mutation compromises the dynein-dynactin retrograde motor complex and causes disruptions to the adult eye and the CNS, including sensory neurons and the formation of the giant fiber system neural circuit.</p> <p>Results</p> <p>We performed a 2-stage genetic screen to identify mutations that modified phenotypes caused by over-expression of a dominant-negative Glued protein. We screened over 34,000 flies and isolated 41 mutations that enhanced or suppressed an eye phenotype. Of these, 12 were assayed for interactions in the giant fiber system by which they altered a giant fiber morphological phenotype and/or altered synaptic function between the giant fiber and the tergotrochanteral muscle motorneuron. Six showed interactions including a new allele of <it>atypical protein kinase C </it>(<it>aPKC</it>). We show that this cell polarity regulator interacts with <it>Glued </it>during central synapse formation. We have mapped the five other interacting mutations to discrete chromosomal regions.</p> <p>Conclusion</p> <p>Our results show that an efficient way to screen for genes involved in central synapse formation is to use a two-step strategy in which a screen for altered eye morphology precedes the analysis of central synaptogenesis. This has highlighted a role for <it>aPKC </it>in the formation of an identified central synapse.</p>http://www.biomedcentral.com/1471-2156/10/77 |
| spellingShingle | Ma Lisha Johns Louise Allen Marcus A modifier screen in the <it>Drosophila </it>eye reveals that <it>aPKC </it>interacts with <it>Glued </it>during central synapse formation BMC Genetics |
| title | A modifier screen in the <it>Drosophila </it>eye reveals that <it>aPKC </it>interacts with <it>Glued </it>during central synapse formation |
| title_full | A modifier screen in the <it>Drosophila </it>eye reveals that <it>aPKC </it>interacts with <it>Glued </it>during central synapse formation |
| title_fullStr | A modifier screen in the <it>Drosophila </it>eye reveals that <it>aPKC </it>interacts with <it>Glued </it>during central synapse formation |
| title_full_unstemmed | A modifier screen in the <it>Drosophila </it>eye reveals that <it>aPKC </it>interacts with <it>Glued </it>during central synapse formation |
| title_short | A modifier screen in the <it>Drosophila </it>eye reveals that <it>aPKC </it>interacts with <it>Glued </it>during central synapse formation |
| title_sort | modifier screen in the it drosophila it eye reveals that it apkc it interacts with it glued it during central synapse formation |
| url | http://www.biomedcentral.com/1471-2156/10/77 |
| work_keys_str_mv | AT malisha amodifierscreenintheitdrosophilaiteyerevealsthatitapkcitinteractswithitglueditduringcentralsynapseformation AT johnslouise amodifierscreenintheitdrosophilaiteyerevealsthatitapkcitinteractswithitglueditduringcentralsynapseformation AT allenmarcus amodifierscreenintheitdrosophilaiteyerevealsthatitapkcitinteractswithitglueditduringcentralsynapseformation AT malisha modifierscreenintheitdrosophilaiteyerevealsthatitapkcitinteractswithitglueditduringcentralsynapseformation AT johnslouise modifierscreenintheitdrosophilaiteyerevealsthatitapkcitinteractswithitglueditduringcentralsynapseformation AT allenmarcus modifierscreenintheitdrosophilaiteyerevealsthatitapkcitinteractswithitglueditduringcentralsynapseformation |