Arbuscular mycorrhiza induces low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and heat shock transcription factor expression
Arbuscular mycorrhizal fungi (AMF) have important roles in enhancing drought tolerance of host plants, but it is not clear whether and how AMF increase drought tolerance in walnut (Juglans regia). We hypothesized that AMF could activate antioxidant defense systems and heat shock transcription factor...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1089420/full |
| _version_ | 1811215585598504960 |
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| author | Wen-Ya Ma Qiu-Yun Qin Ying-Ning Zou Kamil Kuča Bhoopander Giri Qiang-Sheng Wu Qiang-Sheng Wu Abeer Hashem Al-Bandari Fahad Al-Arjani Khalid F. Almutairi Elsayed Fathi Abd_Allah Yong-Jie Xu Yong-Jie Xu |
| author_facet | Wen-Ya Ma Qiu-Yun Qin Ying-Ning Zou Kamil Kuča Bhoopander Giri Qiang-Sheng Wu Qiang-Sheng Wu Abeer Hashem Al-Bandari Fahad Al-Arjani Khalid F. Almutairi Elsayed Fathi Abd_Allah Yong-Jie Xu Yong-Jie Xu |
| author_sort | Wen-Ya Ma |
| collection | DOAJ |
| description | Arbuscular mycorrhizal fungi (AMF) have important roles in enhancing drought tolerance of host plants, but it is not clear whether and how AMF increase drought tolerance in walnut (Juglans regia). We hypothesized that AMF could activate antioxidant defense systems and heat shock transcription factors (Hsfs) transcription levels to alleviate oxidative damage caused by drought. The walnut variety ‘Liaohe No. 1’ was inoculated with Diversispora spurca and exposed to well-watered (WW, 75% of the maximum soil water capacity) and drought stress (DS, 50% of the maximum soil water capacity) for 6 weeks. Plant growth, antioxidant defense systems, and expressions of five JrHsfs in leaves were studied. Such drought treatment inhibited root mycorrhizal colonization, while plant growth performance was still improved by AMF inoculation. Mycorrhizal fungal inoculation triggered the increase in soluble protein, glutathione (GSH), ascorbic acid (ASC), and total ASC contents and ascorbic peroxidase and glutathione reductase activities, along with lower hydrogen peroxide (H2O2), superoxide anion radical (O2•−), and malondialdehyde (MDA) levels, compared with non-inoculation under drought. Mycorrhizal plants also recorded higher peroxidase, catalase, and superoxide dismutase activities than non-mycorrhizal plants under drought. The expression of JrHsf03, JrHsf05, JrHsf20, JrHsf22, and JrHsf24 was up-regulated under WW by AMF, while the expression of JrHsf03, JrHsf22, and JrHsf24 were up-regulated only under drought by AMF. It is concluded that D. spurca induced low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and part Hsfs expressions. |
| first_indexed | 2024-04-12T06:25:05Z |
| format | Article |
| id | doaj.art-acb7ed8d23e94aeb86420ed31a76c6b6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T06:25:05Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-acb7ed8d23e94aeb86420ed31a76c6b62022-12-22T03:44:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10894201089420Arbuscular mycorrhiza induces low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and heat shock transcription factor expressionWen-Ya Ma0Qiu-Yun Qin1Ying-Ning Zou2Kamil Kuča3Bhoopander Giri4Qiang-Sheng Wu5Qiang-Sheng Wu6Abeer Hashem7Al-Bandari Fahad Al-Arjani8Khalid F. Almutairi9Elsayed Fathi Abd_Allah10Yong-Jie Xu11Yong-Jie Xu12Tibet Plateau Walnut Industry Research Institute/College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, ChinaTibet Plateau Walnut Industry Research Institute/College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, ChinaTibet Plateau Walnut Industry Research Institute/College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, ChinaDepartment of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, CzechiaDepartment of Botany, Swami Shraddhanand College, University of Delhi, New Delhi, IndiaTibet Plateau Walnut Industry Research Institute/College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, ChinaDepartment of Chemistry, Faculty of Science, University of Hradec Kralove, Hradec Kralove, CzechiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi ArabiaPlant Production Department, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi ArabiaPlant Production Department, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi ArabiaHubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang, ChinaHubei Academy of Forestry, Wuhan, ChinaArbuscular mycorrhizal fungi (AMF) have important roles in enhancing drought tolerance of host plants, but it is not clear whether and how AMF increase drought tolerance in walnut (Juglans regia). We hypothesized that AMF could activate antioxidant defense systems and heat shock transcription factors (Hsfs) transcription levels to alleviate oxidative damage caused by drought. The walnut variety ‘Liaohe No. 1’ was inoculated with Diversispora spurca and exposed to well-watered (WW, 75% of the maximum soil water capacity) and drought stress (DS, 50% of the maximum soil water capacity) for 6 weeks. Plant growth, antioxidant defense systems, and expressions of five JrHsfs in leaves were studied. Such drought treatment inhibited root mycorrhizal colonization, while plant growth performance was still improved by AMF inoculation. Mycorrhizal fungal inoculation triggered the increase in soluble protein, glutathione (GSH), ascorbic acid (ASC), and total ASC contents and ascorbic peroxidase and glutathione reductase activities, along with lower hydrogen peroxide (H2O2), superoxide anion radical (O2•−), and malondialdehyde (MDA) levels, compared with non-inoculation under drought. Mycorrhizal plants also recorded higher peroxidase, catalase, and superoxide dismutase activities than non-mycorrhizal plants under drought. The expression of JrHsf03, JrHsf05, JrHsf20, JrHsf22, and JrHsf24 was up-regulated under WW by AMF, while the expression of JrHsf03, JrHsf22, and JrHsf24 were up-regulated only under drought by AMF. It is concluded that D. spurca induced low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and part Hsfs expressions.https://www.frontiersin.org/articles/10.3389/fpls.2022.1089420/fullarbuscular mycorrhizaheat shock transcription factorreactive oxygen speciessymbiosiswater stress |
| spellingShingle | Wen-Ya Ma Qiu-Yun Qin Ying-Ning Zou Kamil Kuča Bhoopander Giri Qiang-Sheng Wu Qiang-Sheng Wu Abeer Hashem Al-Bandari Fahad Al-Arjani Khalid F. Almutairi Elsayed Fathi Abd_Allah Yong-Jie Xu Yong-Jie Xu Arbuscular mycorrhiza induces low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and heat shock transcription factor expression Frontiers in Plant Science arbuscular mycorrhiza heat shock transcription factor reactive oxygen species symbiosis water stress |
| title | Arbuscular mycorrhiza induces low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and heat shock transcription factor expression |
| title_full | Arbuscular mycorrhiza induces low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and heat shock transcription factor expression |
| title_fullStr | Arbuscular mycorrhiza induces low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and heat shock transcription factor expression |
| title_full_unstemmed | Arbuscular mycorrhiza induces low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and heat shock transcription factor expression |
| title_short | Arbuscular mycorrhiza induces low oxidative burst in drought-stressed walnut through activating antioxidant defense systems and heat shock transcription factor expression |
| title_sort | arbuscular mycorrhiza induces low oxidative burst in drought stressed walnut through activating antioxidant defense systems and heat shock transcription factor expression |
| topic | arbuscular mycorrhiza heat shock transcription factor reactive oxygen species symbiosis water stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1089420/full |
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