Chemical composition and antioxidant activity of petroleum ether fraction of Rosmarinus officinalis
The presented study examines the chemical composition and antioxidant activity of the petroleum ether fraction of Rosmarinus officinalis (PEF-RO), which was obtained via 75 % ethanol extraction followed by petroleum ether extraction. The obtained fractions were analyzed by gas chromatography-mass sp...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023085249 |
| _version_ | 1827616030169497600 |
|---|---|
| author | Xiaojing Shen Ming Zhou Xingfan Zhu Jiaojiao Zhang Junju Xu Weiwei Jiang |
| author_facet | Xiaojing Shen Ming Zhou Xingfan Zhu Jiaojiao Zhang Junju Xu Weiwei Jiang |
| author_sort | Xiaojing Shen |
| collection | DOAJ |
| description | The presented study examines the chemical composition and antioxidant activity of the petroleum ether fraction of Rosmarinus officinalis (PEF-RO), which was obtained via 75 % ethanol extraction followed by petroleum ether extraction. The obtained fractions were analyzed by gas chromatography-mass spectrometry (GC-MS). The in vitro antioxidant activity of PEF-RO was investigated using various assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate (ABTS) free radical scavenging, and ferric reducing antioxidant power (FRAP) method. A total of 82 chemical components were successfully identified, totaling 10.06 % of PEF-RO content. The identified components consisted of 24 hydrocarbons, 14 ketones, 16 alcohols, 4 phenols, 14 esters, and 10 other compounds. Notably, verbenone (2.4377 %), vitamin A (0.6854 %), trans-geraniol (0.5998 %), linolenic acid (0.5713 %), and 1,8-eucalyptol (0.5323 %) were the most abundant compounds, and there are many trace components in PEF-RO. PEF-RO's IC50 values of DPPH and ABTS free radical scavenging were determined as 0.36 mg/mL and 0.19 mg/mL, respectively. FRAP-method was employed to measure the total antioxidant energy of PEF-RO, which displayed good antioxidant activity. The obtained data provides the foundation for the comprehensive development and utilization of Rosmarinus officinalis. |
| first_indexed | 2024-03-09T09:19:59Z |
| format | Article |
| id | doaj.art-acb808052b654b0c9acdc38a0cb6c3c7 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:19:59Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-acb808052b654b0c9acdc38a0cb6c3c72023-12-02T07:01:50ZengElsevierHeliyon2405-84402023-11-01911e21316Chemical composition and antioxidant activity of petroleum ether fraction of Rosmarinus officinalisXiaojing Shen0Ming Zhou1Xingfan Zhu2Jiaojiao Zhang3Junju Xu4Weiwei Jiang5College of Science, Yunnan Agricultural University, Kunming, ChinaCollege of Science, Yunnan Agricultural University, Kunming, ChinaCollege of Science, Yunnan Agricultural University, Kunming, ChinaCollege of Science, Yunnan Agricultural University, Kunming, ChinaCollege of Tobacco Science, Yunnan Agricultural University, Kunming, China; Key Laboratory of Sustainable Utilization of Plateau Characteristic Spice Plant Resources, Education Department of Yunnan Province, Kunming, China; Corresponding author. College of Tobacco Science, Yunnan Agricultural University, Kunming, China.College of Science, Yunnan Agricultural University, Kunming, China; Key Laboratory of Sustainable Utilization of Plateau Characteristic Spice Plant Resources, Education Department of Yunnan Province, Kunming, China; Yunnan Characteristic Plant Extraction Laboratory, Kunming, 650106, China; Corresponding author. College of Science, Yunnan Agricultural University, Kunming, China.The presented study examines the chemical composition and antioxidant activity of the petroleum ether fraction of Rosmarinus officinalis (PEF-RO), which was obtained via 75 % ethanol extraction followed by petroleum ether extraction. The obtained fractions were analyzed by gas chromatography-mass spectrometry (GC-MS). The in vitro antioxidant activity of PEF-RO was investigated using various assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate (ABTS) free radical scavenging, and ferric reducing antioxidant power (FRAP) method. A total of 82 chemical components were successfully identified, totaling 10.06 % of PEF-RO content. The identified components consisted of 24 hydrocarbons, 14 ketones, 16 alcohols, 4 phenols, 14 esters, and 10 other compounds. Notably, verbenone (2.4377 %), vitamin A (0.6854 %), trans-geraniol (0.5998 %), linolenic acid (0.5713 %), and 1,8-eucalyptol (0.5323 %) were the most abundant compounds, and there are many trace components in PEF-RO. PEF-RO's IC50 values of DPPH and ABTS free radical scavenging were determined as 0.36 mg/mL and 0.19 mg/mL, respectively. FRAP-method was employed to measure the total antioxidant energy of PEF-RO, which displayed good antioxidant activity. The obtained data provides the foundation for the comprehensive development and utilization of Rosmarinus officinalis.http://www.sciencedirect.com/science/article/pii/S2405844023085249Rosmarinus officinalisChemical compositionAntioxidant |
| spellingShingle | Xiaojing Shen Ming Zhou Xingfan Zhu Jiaojiao Zhang Junju Xu Weiwei Jiang Chemical composition and antioxidant activity of petroleum ether fraction of Rosmarinus officinalis Heliyon Rosmarinus officinalis Chemical composition Antioxidant |
| title | Chemical composition and antioxidant activity of petroleum ether fraction of Rosmarinus officinalis |
| title_full | Chemical composition and antioxidant activity of petroleum ether fraction of Rosmarinus officinalis |
| title_fullStr | Chemical composition and antioxidant activity of petroleum ether fraction of Rosmarinus officinalis |
| title_full_unstemmed | Chemical composition and antioxidant activity of petroleum ether fraction of Rosmarinus officinalis |
| title_short | Chemical composition and antioxidant activity of petroleum ether fraction of Rosmarinus officinalis |
| title_sort | chemical composition and antioxidant activity of petroleum ether fraction of rosmarinus officinalis |
| topic | Rosmarinus officinalis Chemical composition Antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023085249 |
| work_keys_str_mv | AT xiaojingshen chemicalcompositionandantioxidantactivityofpetroleumetherfractionofrosmarinusofficinalis AT mingzhou chemicalcompositionandantioxidantactivityofpetroleumetherfractionofrosmarinusofficinalis AT xingfanzhu chemicalcompositionandantioxidantactivityofpetroleumetherfractionofrosmarinusofficinalis AT jiaojiaozhang chemicalcompositionandantioxidantactivityofpetroleumetherfractionofrosmarinusofficinalis AT junjuxu chemicalcompositionandantioxidantactivityofpetroleumetherfractionofrosmarinusofficinalis AT weiweijiang chemicalcompositionandantioxidantactivityofpetroleumetherfractionofrosmarinusofficinalis |