Responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo-aquic and black soil of North China
This research investigates the impact of long-term nitrogen (N) addition on fluvo-aquic and black soils in north China, with a focus on soil microbial communities and enzyme activities. In each site, there were three N fertilization treatments, i.e., control, moderate-N, and high-N. Phospholipid Fat...
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| Format: | Article |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1249471/full |
| _version_ | 1797741427347685376 |
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| author | Sami Ullah Sami Ullah Muhammad Mohsin Raza Tanveer Abbas Xian Guan Wei Zhou Ping He |
| author_facet | Sami Ullah Sami Ullah Muhammad Mohsin Raza Tanveer Abbas Xian Guan Wei Zhou Ping He |
| author_sort | Sami Ullah |
| collection | DOAJ |
| description | This research investigates the impact of long-term nitrogen (N) addition on fluvo-aquic and black soils in north China, with a focus on soil microbial communities and enzyme activities. In each site, there were three N fertilization treatments, i.e., control, moderate-N, and high-N. Phospholipid Fatty Acid Analysis was employed to analyze the microbial community composition, and enzyme activities related to N, carbon (C), and phosphorus (P) cycling were assessed. The results showed that increasing N fertilization levels led to higher soil organic carbon (SOC) and total N (TN) concentrations, indicating enhanced nutrient availability. N fertilization reduced soil pH across both soils, with a more pronounced acidification effect observed in the black soil. Across both soils, N addition increased maize yield, but the higher crop yield was attained in moderate-N rate compared with high-N rate. Microbial community composition analysis revealed that N fertilization induced shifts in the relative abundances of specific microbial groups. The black soil exhibited pronounced shifts in the microbial groups compared to the fluvo-aquic soil, i.e., decreased fungal abundance and fungi: bacteria ratio in response to N input. In addition, the application of N fertilizer led to an elevated ratio of gram-positive to gram-negative (GP:GN) bacteria, but this effect was observed only in black soil. N fertilization had an impact on the enzyme activities related to C, N, and P cycling in both soil types, but black soil showed more pronounced changes in enzyme activities. Permutational multivariate analysis of variance indicated that soil types rather than N fertilization mediated the response of the soil microbial community and enzyme activities. Partial least square path modeling demonstrated that soil pH was the only key driver impacting soil microbial groups and enzyme activities in both soils. In conclusion, our findings highlighted that N fertilization exerted more pronounced impacts on soil biochemical properties, microbial community composition, and enzyme activities in black soil furthermore, moderate N rate resulted in higher crop productivity over high N rate. |
| first_indexed | 2024-03-12T14:26:33Z |
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| id | doaj.art-acb896d3cde04432bf4f244c25a8ad16 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T14:26:33Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-acb896d3cde04432bf4f244c25a8ad162023-08-18T05:11:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12494711249471Responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo-aquic and black soil of North ChinaSami Ullah0Sami Ullah1Muhammad Mohsin Raza2Tanveer Abbas3Xian Guan4Wei Zhou5Ping He6Ministry of Agriculture Key Laboratory of Plant Nutrition and Fertilizer, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Science, Beijing, ChinaORIC, University of Baltistan, Skardu, PakistanSoil Environment and Chemistry Program, Land Resources Research Institute National Agriculture Research Center, Pakistan Agricultural Research Council, Islamabad, PakistanMinistry of Agriculture Key Laboratory of Plant Nutrition and Fertilizer, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Science, Beijing, ChinaMosaic Fertilizers (Beijing) Co., Ltd, Beijing, ChinaMinistry of Agriculture Key Laboratory of Plant Nutrition and Fertilizer, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Science, Beijing, ChinaMinistry of Agriculture Key Laboratory of Plant Nutrition and Fertilizer, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Science, Beijing, ChinaThis research investigates the impact of long-term nitrogen (N) addition on fluvo-aquic and black soils in north China, with a focus on soil microbial communities and enzyme activities. In each site, there were three N fertilization treatments, i.e., control, moderate-N, and high-N. Phospholipid Fatty Acid Analysis was employed to analyze the microbial community composition, and enzyme activities related to N, carbon (C), and phosphorus (P) cycling were assessed. The results showed that increasing N fertilization levels led to higher soil organic carbon (SOC) and total N (TN) concentrations, indicating enhanced nutrient availability. N fertilization reduced soil pH across both soils, with a more pronounced acidification effect observed in the black soil. Across both soils, N addition increased maize yield, but the higher crop yield was attained in moderate-N rate compared with high-N rate. Microbial community composition analysis revealed that N fertilization induced shifts in the relative abundances of specific microbial groups. The black soil exhibited pronounced shifts in the microbial groups compared to the fluvo-aquic soil, i.e., decreased fungal abundance and fungi: bacteria ratio in response to N input. In addition, the application of N fertilizer led to an elevated ratio of gram-positive to gram-negative (GP:GN) bacteria, but this effect was observed only in black soil. N fertilization had an impact on the enzyme activities related to C, N, and P cycling in both soil types, but black soil showed more pronounced changes in enzyme activities. Permutational multivariate analysis of variance indicated that soil types rather than N fertilization mediated the response of the soil microbial community and enzyme activities. Partial least square path modeling demonstrated that soil pH was the only key driver impacting soil microbial groups and enzyme activities in both soils. In conclusion, our findings highlighted that N fertilization exerted more pronounced impacts on soil biochemical properties, microbial community composition, and enzyme activities in black soil furthermore, moderate N rate resulted in higher crop productivity over high N rate.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1249471/fullnitrogen fertilizationsoil microbial community compositionextracellular enzyme activitiesfluvo-aquic soilblack soil |
| spellingShingle | Sami Ullah Sami Ullah Muhammad Mohsin Raza Tanveer Abbas Xian Guan Wei Zhou Ping He Responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo-aquic and black soil of North China Frontiers in Microbiology nitrogen fertilization soil microbial community composition extracellular enzyme activities fluvo-aquic soil black soil |
| title | Responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo-aquic and black soil of North China |
| title_full | Responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo-aquic and black soil of North China |
| title_fullStr | Responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo-aquic and black soil of North China |
| title_full_unstemmed | Responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo-aquic and black soil of North China |
| title_short | Responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo-aquic and black soil of North China |
| title_sort | responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo aquic and black soil of north china |
| topic | nitrogen fertilization soil microbial community composition extracellular enzyme activities fluvo-aquic soil black soil |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1249471/full |
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