Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran
Abstract Stenotrophomonas maltophilia exhibits wide spectrum of fluoroquinolone resistance using different mechanisms as multidrug efflux pumps and Smqnr alleles. Here, the role of smeDEF, smeVWX efflux genes and contribution of Smqnr alleles in the development of fluoroquinolone resistance was asse...
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| Format: | Article |
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Nature Portfolio
2021-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-88977-z |
| _version_ | 1818858293211168768 |
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| author | Akram Azimi Farhad Rezaei Mehdi Yaseri Sirus Jafari Mohammad Rahbar Masoumeh Douraghi |
| author_facet | Akram Azimi Farhad Rezaei Mehdi Yaseri Sirus Jafari Mohammad Rahbar Masoumeh Douraghi |
| author_sort | Akram Azimi |
| collection | DOAJ |
| description | Abstract Stenotrophomonas maltophilia exhibits wide spectrum of fluoroquinolone resistance using different mechanisms as multidrug efflux pumps and Smqnr alleles. Here, the role of smeDEF, smeVWX efflux genes and contribution of Smqnr alleles in the development of fluoroquinolone resistance was assessed. Ciprofloxacin, levofloxacin and moxifloxacin resistance were found in 10.9%, 3.5%, and 1.6% of isolates, respectively. More than four-fold differences in ciprofloxacin MICs were detected in the presence of reserpine and smeD, F, V expression was significantly associated with ciprofloxacin resistance (p = 0.017 for smeD, 0.003 for smeF, and 0.001 for smeV). Smqnr gene was found in 52% of the ciprofloxacin-resistant isolates and Smqnr8 was the most common allele detected. Fluoroquinolone resistance in S. maltophilia clinical isolates was significantly associated with active efflux pumps. There was no correlation between the Smqnr alleles and ciprofloxacin resistance; however, contribution of the Smqnr genes in low-level levofloxacin resistance was revealed. |
| first_indexed | 2024-12-19T08:53:59Z |
| format | Article |
| id | doaj.art-acbb74c319fd49779699549a22cb24af |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T08:53:59Z |
| publishDate | 2021-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-acbb74c319fd49779699549a22cb24af2022-12-21T20:28:39ZengNature PortfolioScientific Reports2045-23222021-05-011111910.1038/s41598-021-88977-zEmergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from IranAkram Azimi0Farhad Rezaei1Mehdi Yaseri2Sirus Jafari3Mohammad Rahbar4Masoumeh Douraghi5Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical SciencesVirology Department, School of Public Health, Tehran University of Medical SciencesDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical SciencesDepartment of Infectious Diseases, Imam-Khomeini Hospital Complex, Tehran University of Medical SciencesReference Health Laboratories, Department of Microbiology, Ministry of HealthDivision of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical SciencesAbstract Stenotrophomonas maltophilia exhibits wide spectrum of fluoroquinolone resistance using different mechanisms as multidrug efflux pumps and Smqnr alleles. Here, the role of smeDEF, smeVWX efflux genes and contribution of Smqnr alleles in the development of fluoroquinolone resistance was assessed. Ciprofloxacin, levofloxacin and moxifloxacin resistance were found in 10.9%, 3.5%, and 1.6% of isolates, respectively. More than four-fold differences in ciprofloxacin MICs were detected in the presence of reserpine and smeD, F, V expression was significantly associated with ciprofloxacin resistance (p = 0.017 for smeD, 0.003 for smeF, and 0.001 for smeV). Smqnr gene was found in 52% of the ciprofloxacin-resistant isolates and Smqnr8 was the most common allele detected. Fluoroquinolone resistance in S. maltophilia clinical isolates was significantly associated with active efflux pumps. There was no correlation between the Smqnr alleles and ciprofloxacin resistance; however, contribution of the Smqnr genes in low-level levofloxacin resistance was revealed.https://doi.org/10.1038/s41598-021-88977-z |
| spellingShingle | Akram Azimi Farhad Rezaei Mehdi Yaseri Sirus Jafari Mohammad Rahbar Masoumeh Douraghi Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran Scientific Reports |
| title | Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran |
| title_full | Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran |
| title_fullStr | Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran |
| title_full_unstemmed | Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran |
| title_short | Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran |
| title_sort | emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of stenotrophomonas maltophilia from iran |
| url | https://doi.org/10.1038/s41598-021-88977-z |
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