Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET
Exploring the spatiotemporal dynamics of biomolecules on a single-molecule level requires innovative ways to make them spectroscopically visible. Fluorescence resonance energy transfer (FRET) uses a pair of organic dyes as reporters to measure distances along a predefined biomolecular reac...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2019-04-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://www.chimia.ch/chimia/article/view/1224 |
| _version_ | 1818295736779931648 |
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| author | Fabio D. Steffen Richard Börner Eva Freisinger Roland K. O. Sigel |
| author_facet | Fabio D. Steffen Richard Börner Eva Freisinger Roland K. O. Sigel |
| author_sort | Fabio D. Steffen |
| collection | DOAJ |
| description |
Exploring the spatiotemporal dynamics of biomolecules on a single-molecule level requires innovative ways to make them spectroscopically visible. Fluorescence resonance energy transfer (FRET) uses a pair of organic dyes as reporters to measure distances along a predefined biomolecular
reaction coordinate. For this nanoscopic ruler to work, the fluorescent labels need to be coupled onto the molecule of interest in a bioorthogonal and site-selective manner. Tagging large non-coding RNAs with single-nucleotide precision is an open challenge. Here we summarize current strategies
in labeling riboswitches and ribozymes for fluorescence spectroscopy and FRET in particular. A special focus lies on our recently developed, DNA-guided approach that inserts two fluorophores through a stepwise process of templated functionality transfer and click chemistry.
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| first_indexed | 2024-12-13T03:52:23Z |
| format | Article |
| id | doaj.art-acc43f3d087e470a9558478478a9b154 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-12-13T03:52:23Z |
| publishDate | 2019-04-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-acc43f3d087e470a9558478478a9b1542022-12-22T00:00:41ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242019-04-0173410.2533/chimia.2019.257Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRETFabio D. Steffen0Richard Börner1Eva Freisinger2Roland K. O. Sigel3Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, SCS-Metrohm Award for best oral presentation in Medicinal Chemistry & Chemical Biology;, Email: fabio.steffen@chem.uzh.chDepartment of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 ZurichDepartment of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 ZurichDepartment of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich;, Email: roland.sigel@chem.uzh.ch Exploring the spatiotemporal dynamics of biomolecules on a single-molecule level requires innovative ways to make them spectroscopically visible. Fluorescence resonance energy transfer (FRET) uses a pair of organic dyes as reporters to measure distances along a predefined biomolecular reaction coordinate. For this nanoscopic ruler to work, the fluorescent labels need to be coupled onto the molecule of interest in a bioorthogonal and site-selective manner. Tagging large non-coding RNAs with single-nucleotide precision is an open challenge. Here we summarize current strategies in labeling riboswitches and ribozymes for fluorescence spectroscopy and FRET in particular. A special focus lies on our recently developed, DNA-guided approach that inserts two fluorophores through a stepwise process of templated functionality transfer and click chemistry. https://www.chimia.ch/chimia/article/view/1224BioorthogonalFluorescenceNucleic acidsRiboswitchSpectroscopy |
| spellingShingle | Fabio D. Steffen Richard Börner Eva Freisinger Roland K. O. Sigel Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET CHIMIA Bioorthogonal Fluorescence Nucleic acids Riboswitch Spectroscopy |
| title | Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET |
| title_full | Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET |
| title_fullStr | Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET |
| title_full_unstemmed | Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET |
| title_short | Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET |
| title_sort | stick flick click dna guided fluorescent labeling of long rna for single molecule fret |
| topic | Bioorthogonal Fluorescence Nucleic acids Riboswitch Spectroscopy |
| url | https://www.chimia.ch/chimia/article/view/1224 |
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