Unraveling the Mode of Action of <i>Cordyceps fumosorosea</i>: Potential Biocontrol Agent against <i>Plutella xylostella</i> (Lepidoptera: Plutellidae)
The entomopathogenic fungus, <i>Cordyceps fumosorosea</i> is a potential eco-friendly biocontrol agent. The present study revealed the entire course of infection of <i>P. xylostella</i> by <i>C. fumosorosea</i> with particular reference to cuticular penetration. C...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/12/2/179 |
| _version_ | 1797396067559407616 |
|---|---|
| author | Yanyuan Lei Abid Hussain Zhaoying Guan Desen Wang Waqar Jaleel Lihua Lyu Yurong He |
| author_facet | Yanyuan Lei Abid Hussain Zhaoying Guan Desen Wang Waqar Jaleel Lihua Lyu Yurong He |
| author_sort | Yanyuan Lei |
| collection | DOAJ |
| description | The entomopathogenic fungus, <i>Cordyceps fumosorosea</i> is a potential eco-friendly biocontrol agent. The present study revealed the entire course of infection of <i>P. xylostella</i> by <i>C. fumosorosea</i> with particular reference to cuticular penetration. Comparative studies on the infection of <i>Plutella xylostella</i> larvae by two strains of <i>C. fumosorosea</i> with different pathogenicity were carried out using light, scanning, and transmission electron microscopy. We found that <i>C. fumosorosea</i> tended to adhere to the cuticle surfaces containing protrusions. Although conidia of the lower pathogenic strain IFCF-D58 germinated, they failed to penetrate and complete the development cycle. In contrast, the higher pathogenic strain IFCF01 began to germinate within 4 h and attached to the cuticle by a thin mucilaginous matrix within 8 h post-inoculation. After 24 h post-inoculation, germ tubes and penetrating hyphae reached the cuticular epidermis and began to enter the haemocoel. Within 36 h post-inoculation, the hyphal bodies colonized the body cavity. Hyphae penetrated from inside to outside of the body after 48 h and sporulated the cadavers. After 72 h post-inoculation, numerous conidia emerged and the mycelial covered the entire cuticular surface. The two strains showed similarities in terms of conidial size and germination rate. However, IFCF-D58 exhibited significantly fewer appressoria and longer penetrating hyphae compared to the more infective IFCF01 on all surface topographies. The current pathogen invasion sequence of events suggested that the aggressive growth and propagation along with rapid and massive in vivo production of blastospores facilitate the conidia of IFCF01 to quickly overcome the diamondback moth’s defense mechanism. |
| first_indexed | 2024-03-09T00:45:55Z |
| format | Article |
| id | doaj.art-acc497e8b10e4a8eada690959a792a68 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T00:45:55Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-acc497e8b10e4a8eada690959a792a682023-12-11T17:33:44ZengMDPI AGInsects2075-44502021-02-0112217910.3390/insects12020179Unraveling the Mode of Action of <i>Cordyceps fumosorosea</i>: Potential Biocontrol Agent against <i>Plutella xylostella</i> (Lepidoptera: Plutellidae)Yanyuan Lei0Abid Hussain1Zhaoying Guan2Desen Wang3Waqar Jaleel4Lihua Lyu5Yurong He6Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaInstitute of Research and Consultancy, King Faisal University, Hofuf 31982, Saudi ArabiaSchool of Applied Biology, Shenzhen Institute of Technology, Shenzhen 518116, ChinaDepartment of Entomology, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaDepartment of Entomology, South China Agricultural University, Guangzhou 510642, ChinaThe entomopathogenic fungus, <i>Cordyceps fumosorosea</i> is a potential eco-friendly biocontrol agent. The present study revealed the entire course of infection of <i>P. xylostella</i> by <i>C. fumosorosea</i> with particular reference to cuticular penetration. Comparative studies on the infection of <i>Plutella xylostella</i> larvae by two strains of <i>C. fumosorosea</i> with different pathogenicity were carried out using light, scanning, and transmission electron microscopy. We found that <i>C. fumosorosea</i> tended to adhere to the cuticle surfaces containing protrusions. Although conidia of the lower pathogenic strain IFCF-D58 germinated, they failed to penetrate and complete the development cycle. In contrast, the higher pathogenic strain IFCF01 began to germinate within 4 h and attached to the cuticle by a thin mucilaginous matrix within 8 h post-inoculation. After 24 h post-inoculation, germ tubes and penetrating hyphae reached the cuticular epidermis and began to enter the haemocoel. Within 36 h post-inoculation, the hyphal bodies colonized the body cavity. Hyphae penetrated from inside to outside of the body after 48 h and sporulated the cadavers. After 72 h post-inoculation, numerous conidia emerged and the mycelial covered the entire cuticular surface. The two strains showed similarities in terms of conidial size and germination rate. However, IFCF-D58 exhibited significantly fewer appressoria and longer penetrating hyphae compared to the more infective IFCF01 on all surface topographies. The current pathogen invasion sequence of events suggested that the aggressive growth and propagation along with rapid and massive in vivo production of blastospores facilitate the conidia of IFCF01 to quickly overcome the diamondback moth’s defense mechanism.https://www.mdpi.com/2075-4450/12/2/179<i>Cordyceps fumosorosea</i>diamondback mothSEMelectron microscopycuticleconidium |
| spellingShingle | Yanyuan Lei Abid Hussain Zhaoying Guan Desen Wang Waqar Jaleel Lihua Lyu Yurong He Unraveling the Mode of Action of <i>Cordyceps fumosorosea</i>: Potential Biocontrol Agent against <i>Plutella xylostella</i> (Lepidoptera: Plutellidae) Insects <i>Cordyceps fumosorosea</i> diamondback moth SEM electron microscopy cuticle conidium |
| title | Unraveling the Mode of Action of <i>Cordyceps fumosorosea</i>: Potential Biocontrol Agent against <i>Plutella xylostella</i> (Lepidoptera: Plutellidae) |
| title_full | Unraveling the Mode of Action of <i>Cordyceps fumosorosea</i>: Potential Biocontrol Agent against <i>Plutella xylostella</i> (Lepidoptera: Plutellidae) |
| title_fullStr | Unraveling the Mode of Action of <i>Cordyceps fumosorosea</i>: Potential Biocontrol Agent against <i>Plutella xylostella</i> (Lepidoptera: Plutellidae) |
| title_full_unstemmed | Unraveling the Mode of Action of <i>Cordyceps fumosorosea</i>: Potential Biocontrol Agent against <i>Plutella xylostella</i> (Lepidoptera: Plutellidae) |
| title_short | Unraveling the Mode of Action of <i>Cordyceps fumosorosea</i>: Potential Biocontrol Agent against <i>Plutella xylostella</i> (Lepidoptera: Plutellidae) |
| title_sort | unraveling the mode of action of i cordyceps fumosorosea i potential biocontrol agent against i plutella xylostella i lepidoptera plutellidae |
| topic | <i>Cordyceps fumosorosea</i> diamondback moth SEM electron microscopy cuticle conidium |
| url | https://www.mdpi.com/2075-4450/12/2/179 |
| work_keys_str_mv | AT yanyuanlei unravelingthemodeofactionoficordycepsfumosoroseaipotentialbiocontrolagentagainstiplutellaxylostellailepidopteraplutellidae AT abidhussain unravelingthemodeofactionoficordycepsfumosoroseaipotentialbiocontrolagentagainstiplutellaxylostellailepidopteraplutellidae AT zhaoyingguan unravelingthemodeofactionoficordycepsfumosoroseaipotentialbiocontrolagentagainstiplutellaxylostellailepidopteraplutellidae AT desenwang unravelingthemodeofactionoficordycepsfumosoroseaipotentialbiocontrolagentagainstiplutellaxylostellailepidopteraplutellidae AT waqarjaleel unravelingthemodeofactionoficordycepsfumosoroseaipotentialbiocontrolagentagainstiplutellaxylostellailepidopteraplutellidae AT lihualyu unravelingthemodeofactionoficordycepsfumosoroseaipotentialbiocontrolagentagainstiplutellaxylostellailepidopteraplutellidae AT yuronghe unravelingthemodeofactionoficordycepsfumosoroseaipotentialbiocontrolagentagainstiplutellaxylostellailepidopteraplutellidae |