HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury
Junyang Chen,1 Xuefeng Li,1 Feng Zhao,2 Yubo Hu1 1Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of China; 2Department of Operating Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, Peopl...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-04-01
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| Series: | Clinical Interventions in Aging |
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| Online Access: | https://www.dovepress.com/hotairmir-17-5p-axis-is-involved-in-the-propofol-mediated-cardioprotec-peer-reviewed-article-CIA |
| _version_ | 1818751585141915648 |
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| author | Chen J Li X Zhao F Hu Y |
| author_facet | Chen J Li X Zhao F Hu Y |
| author_sort | Chen J |
| collection | DOAJ |
| description | Junyang Chen,1 Xuefeng Li,1 Feng Zhao,2 Yubo Hu1 1Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of China; 2Department of Operating Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of ChinaCorrespondence: Yubo HuDepartment of Anesthesiology, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun, Jilin 130033, People’s Republic of ChinaEmail huyubo717@jlu.edu.cnBackground: Propofol (PPF) ameliorates ischemia/reperfusion (I/R) injury in multiple organs by reducing apoptosis and release of pro-inflammatory cytokines. This study aims to explore the mechanism of PPF in attenuating myocardial ischemia-reperfusion injury (MIRI).Materials and Methods: Rat MIRI model was established, and PPF pre-treatment was performed 10 min before I/R. H9c2 cardiomyocytes treated with hypoxia/reoxygenation (H/R) were used to establish an in vitro model. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to evaluate HOTAIR and miR-17-5p expression levels. Flow cytometry was employed to detect the apoptosis of H9c2 cells. The interaction between HOTAIR and miR-17-5p was determined by bioinformatics analysis, luciferase reporter gene analysis, and RNA immunoprecipitation experiments. STAT3 and p-STAT3 expressions were detected by Western blot.Results: PPF pre-treatment significantly reduced creatine kinase isoenzyme (CK-MB) and serum lactate dehydrogenase (LDH) levels in the serum of the rats with MIRI. PPF pre-treatment remarkably up-regulated HOTAIR expression and down-regulated miR-17-5p expression in both in vivo and in vitro models. HOTAIR adsorbed miR-17-5p to repress the expression of miR-17-5p. PPF pre-treatment markedly inhibited cardiomyocyte apoptosis induced by I/R or H/R. HOTAIR knockdown could partially reverse the protective effects of PPF on MIRI. HOTAIR could activate STAT3 signaling via repressing miR-17-5p expression.Conclusion: PPF protects the MIRI by modulating the HOTAIR/miR-17-5p/STAT3 axis.Keywords: PPF, HOTAIR, miR-17-5p |
| first_indexed | 2024-12-18T04:37:54Z |
| format | Article |
| id | doaj.art-acc5dda299af41bca3f1945e52c418da |
| institution | Directory Open Access Journal |
| issn | 1178-1998 |
| language | English |
| last_indexed | 2024-12-18T04:37:54Z |
| publishDate | 2021-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical Interventions in Aging |
| spelling | doaj.art-acc5dda299af41bca3f1945e52c418da2022-12-21T21:20:49ZengDove Medical PressClinical Interventions in Aging1178-19982021-04-01Volume 1662163263922HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion InjuryChen JLi XZhao FHu YJunyang Chen,1 Xuefeng Li,1 Feng Zhao,2 Yubo Hu1 1Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of China; 2Department of Operating Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People’s Republic of ChinaCorrespondence: Yubo HuDepartment of Anesthesiology, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun, Jilin 130033, People’s Republic of ChinaEmail huyubo717@jlu.edu.cnBackground: Propofol (PPF) ameliorates ischemia/reperfusion (I/R) injury in multiple organs by reducing apoptosis and release of pro-inflammatory cytokines. This study aims to explore the mechanism of PPF in attenuating myocardial ischemia-reperfusion injury (MIRI).Materials and Methods: Rat MIRI model was established, and PPF pre-treatment was performed 10 min before I/R. H9c2 cardiomyocytes treated with hypoxia/reoxygenation (H/R) were used to establish an in vitro model. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to evaluate HOTAIR and miR-17-5p expression levels. Flow cytometry was employed to detect the apoptosis of H9c2 cells. The interaction between HOTAIR and miR-17-5p was determined by bioinformatics analysis, luciferase reporter gene analysis, and RNA immunoprecipitation experiments. STAT3 and p-STAT3 expressions were detected by Western blot.Results: PPF pre-treatment significantly reduced creatine kinase isoenzyme (CK-MB) and serum lactate dehydrogenase (LDH) levels in the serum of the rats with MIRI. PPF pre-treatment remarkably up-regulated HOTAIR expression and down-regulated miR-17-5p expression in both in vivo and in vitro models. HOTAIR adsorbed miR-17-5p to repress the expression of miR-17-5p. PPF pre-treatment markedly inhibited cardiomyocyte apoptosis induced by I/R or H/R. HOTAIR knockdown could partially reverse the protective effects of PPF on MIRI. HOTAIR could activate STAT3 signaling via repressing miR-17-5p expression.Conclusion: PPF protects the MIRI by modulating the HOTAIR/miR-17-5p/STAT3 axis.Keywords: PPF, HOTAIR, miR-17-5phttps://www.dovepress.com/hotairmir-17-5p-axis-is-involved-in-the-propofol-mediated-cardioprotec-peer-reviewed-article-CIAppfhotairmir-17-5p |
| spellingShingle | Chen J Li X Zhao F Hu Y HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury Clinical Interventions in Aging ppf hotair mir-17-5p |
| title | HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury |
| title_full | HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury |
| title_fullStr | HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury |
| title_full_unstemmed | HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury |
| title_short | HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury |
| title_sort | hotair mir 17 5p axis is involved in the propofol mediated cardioprotection against ischemia reperfusion injury |
| topic | ppf hotair mir-17-5p |
| url | https://www.dovepress.com/hotairmir-17-5p-axis-is-involved-in-the-propofol-mediated-cardioprotec-peer-reviewed-article-CIA |
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