Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity
Summary Next to d‐glucose, the pentoses l‐arabinose and d‐xylose are the main monosaccharide components of plant cell wall polysaccharides and are therefore of major importance in biotechnological applications that use plant biomass as a substrate. Pentose catabolism is one of the best‐studied pathw...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-11-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13790 |
| _version_ | 1818402090666426368 |
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| author | Tania Chroumpi Mao Peng Maria Victoria Aguilar‐Pontes Astrid Müller Mei Wang Juying Yan Anna Lipzen Vivian Ng Igor V. Grigoriev Miia R. Mäkelä Ronald P. deVries |
| author_facet | Tania Chroumpi Mao Peng Maria Victoria Aguilar‐Pontes Astrid Müller Mei Wang Juying Yan Anna Lipzen Vivian Ng Igor V. Grigoriev Miia R. Mäkelä Ronald P. deVries |
| author_sort | Tania Chroumpi |
| collection | DOAJ |
| description | Summary Next to d‐glucose, the pentoses l‐arabinose and d‐xylose are the main monosaccharide components of plant cell wall polysaccharides and are therefore of major importance in biotechnological applications that use plant biomass as a substrate. Pentose catabolism is one of the best‐studied pathways of primary metabolism of Aspergillus niger, and an initial outline of this pathway with individual enzymes covering each step of the pathway has been previously established. However, although growth on l‐arabinose and/or d‐xylose of most pentose catabolic pathway (PCP) single deletion mutants of A. niger has been shown to be negatively affected, it was not abolished, suggesting the involvement of additional enzymes. Detailed analysis of the single deletion mutants of the known A. niger PCP genes led to the identification of additional genes involved in the pathway. These results reveal a high level of complexity and redundancy in this pathway, emphasizing the need for a comprehensive understanding of metabolic pathways before entering metabolic engineering of such pathways for the generation of more efficient fungal cell factories. |
| first_indexed | 2024-12-14T08:02:50Z |
| format | Article |
| id | doaj.art-acc8dbe60744405db693deec80e04222 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-14T08:02:50Z |
| publishDate | 2021-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-acc8dbe60744405db693deec80e042222022-12-21T23:10:19ZengWileyMicrobial Biotechnology1751-79152021-11-011462525253710.1111/1751-7915.13790Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversityTania Chroumpi0Mao Peng1Maria Victoria Aguilar‐Pontes2Astrid Müller3Mei Wang4Juying Yan5Anna Lipzen6Vivian Ng7Igor V. Grigoriev8Miia R. Mäkelä9Ronald P. deVries10Fungal Physiology Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology Utrecht University Uppsalalaan 8 Utrecht 3584 CT The NetherlandsFungal Physiology Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology Utrecht University Uppsalalaan 8 Utrecht 3584 CT The NetherlandsFungal Physiology Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology Utrecht University Uppsalalaan 8 Utrecht 3584 CT The NetherlandsFungal Physiology Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology Utrecht University Uppsalalaan 8 Utrecht 3584 CT The NetherlandsUS Department of Energy Joint Genome Institute Lawrence Berkeley National Laboratory 1 Cyclotron Road Berkeley CA 94720 USAUS Department of Energy Joint Genome Institute Lawrence Berkeley National Laboratory 1 Cyclotron Road Berkeley CA 94720 USAUS Department of Energy Joint Genome Institute Lawrence Berkeley National Laboratory 1 Cyclotron Road Berkeley CA 94720 USAUS Department of Energy Joint Genome Institute Lawrence Berkeley National Laboratory 1 Cyclotron Road Berkeley CA 94720 USAUS Department of Energy Joint Genome Institute Lawrence Berkeley National Laboratory 1 Cyclotron Road Berkeley CA 94720 USADepartment of Microbiology University of Helsinki P.O. Box 56Viikinkaari 9 Helsinki FinlandFungal Physiology Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology Utrecht University Uppsalalaan 8 Utrecht 3584 CT The NetherlandsSummary Next to d‐glucose, the pentoses l‐arabinose and d‐xylose are the main monosaccharide components of plant cell wall polysaccharides and are therefore of major importance in biotechnological applications that use plant biomass as a substrate. Pentose catabolism is one of the best‐studied pathways of primary metabolism of Aspergillus niger, and an initial outline of this pathway with individual enzymes covering each step of the pathway has been previously established. However, although growth on l‐arabinose and/or d‐xylose of most pentose catabolic pathway (PCP) single deletion mutants of A. niger has been shown to be negatively affected, it was not abolished, suggesting the involvement of additional enzymes. Detailed analysis of the single deletion mutants of the known A. niger PCP genes led to the identification of additional genes involved in the pathway. These results reveal a high level of complexity and redundancy in this pathway, emphasizing the need for a comprehensive understanding of metabolic pathways before entering metabolic engineering of such pathways for the generation of more efficient fungal cell factories.https://doi.org/10.1111/1751-7915.13790 |
| spellingShingle | Tania Chroumpi Mao Peng Maria Victoria Aguilar‐Pontes Astrid Müller Mei Wang Juying Yan Anna Lipzen Vivian Ng Igor V. Grigoriev Miia R. Mäkelä Ronald P. deVries Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity Microbial Biotechnology |
| title | Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity |
| title_full | Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity |
| title_fullStr | Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity |
| title_full_unstemmed | Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity |
| title_short | Revisiting a ‘simple’ fungal metabolic pathway reveals redundancy, complexity and diversity |
| title_sort | revisiting a simple fungal metabolic pathway reveals redundancy complexity and diversity |
| url | https://doi.org/10.1111/1751-7915.13790 |
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