Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species
Background: Gramineae damping-off disease is a growing problem worldwide, which affects a large range of seedlings in nurseries, glasshouses, gardens, crops, forests and untimely generates a heavy economic impact on the agriculture and related sectors. Objectives: The present study was conducted to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/5/985 |
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| author | Hamza Saghrouchni Azeddin El Barnossi Ahmad Mohammad Salamatullah Mohammed Bourhia Abdulhakeem Alzahrani Mohammed Saeed Alkaltham Heba Khalil Alyahya Nour El Houda Tahiri Hamada Imtara Işıl Var |
| author_facet | Hamza Saghrouchni Azeddin El Barnossi Ahmad Mohammad Salamatullah Mohammed Bourhia Abdulhakeem Alzahrani Mohammed Saeed Alkaltham Heba Khalil Alyahya Nour El Houda Tahiri Hamada Imtara Işıl Var |
| author_sort | Hamza Saghrouchni |
| collection | DOAJ |
| description | Background: Gramineae damping-off disease is a growing problem worldwide, which affects a large range of seedlings in nurseries, glasshouses, gardens, crops, forests and untimely generates a heavy economic impact on the agriculture and related sectors. Objectives: The present study was conducted to evaluate the preventive potential of carvacrol on germination of <i>Fusarium oxysporum</i>, <i>Neocosmospora solani</i>, and <i>Microdochium nivale</i> spores as responsible agents for <i>Lolium perenne</i> seeds damping-off disease. Material and methods: Macrodilution method in agar medium, spore germination, spore destruction, and preventive treatment bioassays were used to achieve this goal. Results: The minimum inhibitory concentration (MIC) of carvacrol vs. tested strains existed in the range of 0.25–0.5 mg/mL. Carvacrol used in concentrations ranging from 0.2 to 0.4 mg/mL inhibited the germination of all fungal spores in a dose-dependent manner. Carvacrol showed a very strong sporicidal effect against all studied fungal strains, and this effect was well confirmed by microscopic observations. The percentage of growth inhibition was found to be strictly correlated to carvacrol dose up vs. all strains. Carvacrol increased the emergence of <i>L. perenne</i> seeds when compared to both uninfested and infested seeds. Conclusion: Based on the results obtained, carvacrol fulfills the requirement for being a natural alternative agent to fight Gramineae seedlings’ damping-off caused by fungal species without adverse effects on the plants. |
| first_indexed | 2024-03-10T11:23:12Z |
| format | Article |
| id | doaj.art-acc9c58a398143f8a02859b02878d9e2 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T11:23:12Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-acc9c58a398143f8a02859b02878d9e22023-11-21T19:53:32ZengMDPI AGAgronomy2073-43952021-05-0111598510.3390/agronomy11050985Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal SpeciesHamza Saghrouchni0Azeddin El Barnossi1Ahmad Mohammad Salamatullah2Mohammed Bourhia3Abdulhakeem Alzahrani4Mohammed Saeed Alkaltham5Heba Khalil Alyahya6Nour El Houda Tahiri7Hamada Imtara8Işıl Var9Department of Biotechnology, Institute of Natural and Applied Sciences, Çukurova University, 1380 Adana, TurkeyLaboratory of Biotechnology, Environment, Agri-Food, and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, 30000 Fez, MoroccoDepartment of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaLaboratory of Chemistry, Biochemistry, Nutrition, and Environment, Faculty of Medicine and Pharmacy, University Hassan II, 20000 Casablanca, MoroccoDepartment of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaLaboratory of Physiology, Pharmacology and Environmental Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, 30000 Fez, MoroccoFaculty of Arts and Sciences, Arab American University Palestine, P.O. Box 240, Jenin 44862, PalestineDepartment of Food Engineering, Faculty of Agriculture, Çukurova University, 1380 Adana, TurkeyBackground: Gramineae damping-off disease is a growing problem worldwide, which affects a large range of seedlings in nurseries, glasshouses, gardens, crops, forests and untimely generates a heavy economic impact on the agriculture and related sectors. Objectives: The present study was conducted to evaluate the preventive potential of carvacrol on germination of <i>Fusarium oxysporum</i>, <i>Neocosmospora solani</i>, and <i>Microdochium nivale</i> spores as responsible agents for <i>Lolium perenne</i> seeds damping-off disease. Material and methods: Macrodilution method in agar medium, spore germination, spore destruction, and preventive treatment bioassays were used to achieve this goal. Results: The minimum inhibitory concentration (MIC) of carvacrol vs. tested strains existed in the range of 0.25–0.5 mg/mL. Carvacrol used in concentrations ranging from 0.2 to 0.4 mg/mL inhibited the germination of all fungal spores in a dose-dependent manner. Carvacrol showed a very strong sporicidal effect against all studied fungal strains, and this effect was well confirmed by microscopic observations. The percentage of growth inhibition was found to be strictly correlated to carvacrol dose up vs. all strains. Carvacrol increased the emergence of <i>L. perenne</i> seeds when compared to both uninfested and infested seeds. Conclusion: Based on the results obtained, carvacrol fulfills the requirement for being a natural alternative agent to fight Gramineae seedlings’ damping-off caused by fungal species without adverse effects on the plants.https://www.mdpi.com/2073-4395/11/5/985carvacroldamping-off diseasefungal species<i>Lolium perenne</i> |
| spellingShingle | Hamza Saghrouchni Azeddin El Barnossi Ahmad Mohammad Salamatullah Mohammed Bourhia Abdulhakeem Alzahrani Mohammed Saeed Alkaltham Heba Khalil Alyahya Nour El Houda Tahiri Hamada Imtara Işıl Var Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species Agronomy carvacrol damping-off disease fungal species <i>Lolium perenne</i> |
| title | Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species |
| title_full | Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species |
| title_fullStr | Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species |
| title_full_unstemmed | Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species |
| title_short | Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species |
| title_sort | carvacrol a promising environmentally friendly agent to fight seeds damping off diseases induced by fungal species |
| topic | carvacrol damping-off disease fungal species <i>Lolium perenne</i> |
| url | https://www.mdpi.com/2073-4395/11/5/985 |
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