Evaluation of full length E1 and E2 glycoproteins of HCV expressed in P. pastoris as a protein-based vaccine candidate
Introduction: The development of an effective vaccine against Hepatitis C virus (HCV) is still a target of intensive vaccine research. The HCV envelope proteins E1 and E2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. Different types of expression systems...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2015-11-01
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| Series: | Vaccine Research |
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| Online Access: | http://vacres.pasteur.ac.ir/article-1-70-en.html |
| _version_ | 1818149481604972544 |
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| author | P Rahimi R Solati M Shokri R Vahabpour F Mahmoudizad MR Aghasadeghi F Motevalli Sh Yazdani M Shayestehpour MR Amiran |
| author_facet | P Rahimi R Solati M Shokri R Vahabpour F Mahmoudizad MR Aghasadeghi F Motevalli Sh Yazdani M Shayestehpour MR Amiran |
| author_sort | P Rahimi |
| collection | DOAJ |
| description | Introduction: The development of an effective vaccine against Hepatitis C virus (HCV) is still a target of intensive vaccine research. The HCV envelope proteins E1 and E2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. Different types of expression systems have been used to express these glycoproteins. In this study, an expression system using Pichia pastoris was used to express E1 and E2 in full length. Methods: E1 and E2 regions containing the restriction sites from HCV 1b were separately amplified and cloned into a pPICZAa vector. The km71h strain of P. pastoris was transfected with the confirmed vectors separately using electroporation. The recombinant E1 and E2 proteins were evaluated for their antigenicity in an ELISA test and the induction of humoral immunity in mice. Results: The expression of full length HCV glycoproteins E1 and E2 in P. pastoris strain km71h was successfully achieved and their specific antibody was detected in serum samples from HCV infected patients. Furthermore, the recombinant glycoproteins could elicit a significant humoral immunity in mice as a vaccine candidate. Conclusion: P. pastoris is one of the best eukaryotic expression systems for the production of HCV glycoproteins in full length and the expressed proteins could be used in diagnostic tests such as ELISA. The induction of humoral immune responses in mice should lead to further studies on these glycoproteins for designing an effective vaccine. |
| first_indexed | 2024-12-11T13:07:43Z |
| format | Article |
| id | doaj.art-acca078abc514181adbed89c084ddd26 |
| institution | Directory Open Access Journal |
| issn | 2383-2819 2423-4923 |
| language | English |
| last_indexed | 2024-12-11T13:07:43Z |
| publishDate | 2015-11-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Vaccine Research |
| spelling | doaj.art-acca078abc514181adbed89c084ddd262022-12-22T01:06:17ZengPasteur Institute of IranVaccine Research2383-28192423-49232015-11-01237480Evaluation of full length E1 and E2 glycoproteins of HCV expressed in P. pastoris as a protein-based vaccine candidateP Rahimi0R Solati1M Shokri2R Vahabpour3F Mahmoudizad4MR Aghasadeghi5F Motevalli6Sh Yazdani7M Shayestehpour8MR Amiran9 Introduction: The development of an effective vaccine against Hepatitis C virus (HCV) is still a target of intensive vaccine research. The HCV envelope proteins E1 and E2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. Different types of expression systems have been used to express these glycoproteins. In this study, an expression system using Pichia pastoris was used to express E1 and E2 in full length. Methods: E1 and E2 regions containing the restriction sites from HCV 1b were separately amplified and cloned into a pPICZAa vector. The km71h strain of P. pastoris was transfected with the confirmed vectors separately using electroporation. The recombinant E1 and E2 proteins were evaluated for their antigenicity in an ELISA test and the induction of humoral immunity in mice. Results: The expression of full length HCV glycoproteins E1 and E2 in P. pastoris strain km71h was successfully achieved and their specific antibody was detected in serum samples from HCV infected patients. Furthermore, the recombinant glycoproteins could elicit a significant humoral immunity in mice as a vaccine candidate. Conclusion: P. pastoris is one of the best eukaryotic expression systems for the production of HCV glycoproteins in full length and the expressed proteins could be used in diagnostic tests such as ELISA. The induction of humoral immune responses in mice should lead to further studies on these glycoproteins for designing an effective vaccine.http://vacres.pasteur.ac.ir/article-1-70-en.htmlhcv e1 and e2pichia pastorisyeast expression systemprotein-based vaccine. |
| spellingShingle | P Rahimi R Solati M Shokri R Vahabpour F Mahmoudizad MR Aghasadeghi F Motevalli Sh Yazdani M Shayestehpour MR Amiran Evaluation of full length E1 and E2 glycoproteins of HCV expressed in P. pastoris as a protein-based vaccine candidate Vaccine Research hcv e1 and e2 pichia pastoris yeast expression system protein-based vaccine. |
| title | Evaluation of full length E1 and E2 glycoproteins of HCV expressed in P. pastoris as a protein-based vaccine candidate |
| title_full | Evaluation of full length E1 and E2 glycoproteins of HCV expressed in P. pastoris as a protein-based vaccine candidate |
| title_fullStr | Evaluation of full length E1 and E2 glycoproteins of HCV expressed in P. pastoris as a protein-based vaccine candidate |
| title_full_unstemmed | Evaluation of full length E1 and E2 glycoproteins of HCV expressed in P. pastoris as a protein-based vaccine candidate |
| title_short | Evaluation of full length E1 and E2 glycoproteins of HCV expressed in P. pastoris as a protein-based vaccine candidate |
| title_sort | evaluation of full length e1 and e2 glycoproteins of hcv expressed in p pastoris as a protein based vaccine candidate |
| topic | hcv e1 and e2 pichia pastoris yeast expression system protein-based vaccine. |
| url | http://vacres.pasteur.ac.ir/article-1-70-en.html |
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