Prepulse inhibition in Drosophila melanogaster larvae
The neural mechanisms of psychiatric diseases like autism spectrum disorder and schizophrenia have been intensively studied, and a number of candidate genes have been identified. However, the relationship between genes and neural system functioning remains unclear. Model organisms may serve as a pow...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2018-09-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/7/9/bio034710 |
| _version_ | 1819071082631528448 |
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| author | Yutaro Matsumoto Kazuya Shimizu Kota Arahata Miku Suzuki Akira Shimizu Koki Takei Junji Yamauchi Satoko Hakeda-Suzuki Takashi Suzuki Takako Morimoto |
| author_facet | Yutaro Matsumoto Kazuya Shimizu Kota Arahata Miku Suzuki Akira Shimizu Koki Takei Junji Yamauchi Satoko Hakeda-Suzuki Takashi Suzuki Takako Morimoto |
| author_sort | Yutaro Matsumoto |
| collection | DOAJ |
| description | The neural mechanisms of psychiatric diseases like autism spectrum disorder and schizophrenia have been intensively studied, and a number of candidate genes have been identified. However, the relationship between genes and neural system functioning remains unclear. Model organisms may serve as a powerful tool for addressing this question due to the availability of established genetic tools. Here, we report prepulse inhibition (PPI) in Drosophila larvae for the first time. PPI is a neurological phenomenon found in humans and other organisms and is used in the diagnosis of schizophrenia and other psychiatric disorders. A weaker prestimulus (prepulse) inhibits the reaction to a subsequent strong, startling stimulus (pulse). Using the larval startle response to the buzz of a predator (wasp), we examined PPI in wild-type flies and two mutants: an fmr1 mutant, which is implicated in Fragile X syndrome, and a centaurin gamma 1A (CenG1A) mutant, which is associated with GTPase, PH, ArfGAP, and ANK domains and implicated in autism. Both mutants showed decreased PPI, whereas, interestingly, double mutants showed substantial PPI. The PPI phenomenon described here can provide a useful tool for the study of neural mechanisms of synaptic modification and psychiatric diseases. |
| first_indexed | 2024-12-21T17:16:11Z |
| format | Article |
| id | doaj.art-accb09f9800642f8a6f24f1dacea3b67 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-21T17:16:11Z |
| publishDate | 2018-09-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-accb09f9800642f8a6f24f1dacea3b672022-12-21T18:56:18ZengThe Company of BiologistsBiology Open2046-63902018-09-017910.1242/bio.034710034710Prepulse inhibition in Drosophila melanogaster larvaeYutaro Matsumoto0Kazuya Shimizu1Kota Arahata2Miku Suzuki3Akira Shimizu4Koki Takei5Junji Yamauchi6Satoko Hakeda-Suzuki7Takashi Suzuki8Takako Morimoto9 Laboratory of Molecular Neuroscience and Neurology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Laboratory of Molecular Neuroscience and Neurology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Laboratory of Molecular Neuroscience and Neurology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Laboratory of Molecular Neuroscience and Neurology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Laboratory of Molecular Neuroscience and Neurology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Laboratory of Molecular Neuroscience and Neurology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Laboratory of Molecular Neuroscience and Neurology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan Tokyo Institute of Technology, School of Life Science and Technology, Yokohama, 226-8501, Japan Tokyo Institute of Technology, School of Life Science and Technology, Yokohama, 226-8501, Japan Laboratory of Molecular Neuroscience and Neurology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan The neural mechanisms of psychiatric diseases like autism spectrum disorder and schizophrenia have been intensively studied, and a number of candidate genes have been identified. However, the relationship between genes and neural system functioning remains unclear. Model organisms may serve as a powerful tool for addressing this question due to the availability of established genetic tools. Here, we report prepulse inhibition (PPI) in Drosophila larvae for the first time. PPI is a neurological phenomenon found in humans and other organisms and is used in the diagnosis of schizophrenia and other psychiatric disorders. A weaker prestimulus (prepulse) inhibits the reaction to a subsequent strong, startling stimulus (pulse). Using the larval startle response to the buzz of a predator (wasp), we examined PPI in wild-type flies and two mutants: an fmr1 mutant, which is implicated in Fragile X syndrome, and a centaurin gamma 1A (CenG1A) mutant, which is associated with GTPase, PH, ArfGAP, and ANK domains and implicated in autism. Both mutants showed decreased PPI, whereas, interestingly, double mutants showed substantial PPI. The PPI phenomenon described here can provide a useful tool for the study of neural mechanisms of synaptic modification and psychiatric diseases.http://bio.biologists.org/content/7/9/bio034710Fragile X syndromePsychiatric diseaseStartle responseCentaurin |
| spellingShingle | Yutaro Matsumoto Kazuya Shimizu Kota Arahata Miku Suzuki Akira Shimizu Koki Takei Junji Yamauchi Satoko Hakeda-Suzuki Takashi Suzuki Takako Morimoto Prepulse inhibition in Drosophila melanogaster larvae Biology Open Fragile X syndrome Psychiatric disease Startle response Centaurin |
| title | Prepulse inhibition in Drosophila melanogaster larvae |
| title_full | Prepulse inhibition in Drosophila melanogaster larvae |
| title_fullStr | Prepulse inhibition in Drosophila melanogaster larvae |
| title_full_unstemmed | Prepulse inhibition in Drosophila melanogaster larvae |
| title_short | Prepulse inhibition in Drosophila melanogaster larvae |
| title_sort | prepulse inhibition in drosophila melanogaster larvae |
| topic | Fragile X syndrome Psychiatric disease Startle response Centaurin |
| url | http://bio.biologists.org/content/7/9/bio034710 |
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