Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration
The use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to b...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550415/?tool=EBI |
| _version_ | 1818739250507546624 |
|---|---|
| author | Elia Bosch-Rué Leire Díez-Tercero Raquel Rodríguez-González Begoña María Bosch-Canals Roman A. Perez |
| author_facet | Elia Bosch-Rué Leire Díez-Tercero Raquel Rodríguez-González Begoña María Bosch-Canals Roman A. Perez |
| author_sort | Elia Bosch-Rué |
| collection | DOAJ |
| description | The use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to better fine tune the angiogenic response. On the one hand, gene expression analysis and the assessment of tubular formation structures with human umbilical vein endothelial cells (HUVEC) revealed that high concentrations (10μM) of Cu2+ in early times and lower concentrations (0.1 and 1μM) at later times (day 7) enhanced angiogenic response. On the other hand, higher concentrations (25μM) of Co2+ during all time course increased the angiogenic gene expression and 0.5, 5 and 25μM enhanced the ability to form tubular structures. To further explore synergistic effects combining both ions, the non-toxic concentrations were used simultaneously, although results showed an increased cell toxicity and no improvement of angiogenic response. These results provide useful information for the design of Cu2+ or Co2+ delivery scaffolds in order to release the appropriate concentration during time course for blood vessel stimulation. |
| first_indexed | 2024-12-18T01:21:51Z |
| format | Article |
| id | doaj.art-acce5dfa44834c86b554914382c6c634 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T01:21:51Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-acce5dfa44834c86b554914382c6c6342022-12-21T21:25:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011610Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regenerationElia Bosch-RuéLeire Díez-TerceroRaquel Rodríguez-GonzálezBegoña María Bosch-CanalsRoman A. PerezThe use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to better fine tune the angiogenic response. On the one hand, gene expression analysis and the assessment of tubular formation structures with human umbilical vein endothelial cells (HUVEC) revealed that high concentrations (10μM) of Cu2+ in early times and lower concentrations (0.1 and 1μM) at later times (day 7) enhanced angiogenic response. On the other hand, higher concentrations (25μM) of Co2+ during all time course increased the angiogenic gene expression and 0.5, 5 and 25μM enhanced the ability to form tubular structures. To further explore synergistic effects combining both ions, the non-toxic concentrations were used simultaneously, although results showed an increased cell toxicity and no improvement of angiogenic response. These results provide useful information for the design of Cu2+ or Co2+ delivery scaffolds in order to release the appropriate concentration during time course for blood vessel stimulation.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550415/?tool=EBI |
| spellingShingle | Elia Bosch-Rué Leire Díez-Tercero Raquel Rodríguez-González Begoña María Bosch-Canals Roman A. Perez Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration PLoS ONE |
| title | Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration |
| title_full | Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration |
| title_fullStr | Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration |
| title_full_unstemmed | Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration |
| title_short | Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration |
| title_sort | assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550415/?tool=EBI |
| work_keys_str_mv | AT eliaboschrue assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration AT leiredieztercero assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration AT raquelrodriguezgonzalez assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration AT begonamariaboschcanals assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration AT romanaperez assessingthepotentialroleofcopperandcobaltinstimulatingangiogenesisfortissueregeneration |