Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia
Introduction: In this study, we determined the influence of intrinsic exercise capacity on the vascular adaptive responses to hind limb ischemia. High Capacity Running, HCR; Low Capacity Running, LCR, rats were used to assess intrinsic aerobic capacity effects on adaptive responses to ischemia.Metho...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2021.752955/full |
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| author | Elizabeth Granier Elizabeth Granier Madaniah O. Zakari Madaniah O. Zakari Musaad B. Alsahly Musaad B. Alsahly Lauren G. Koch Steven Britton Laxmansa C. Katwa Robert M. Lust Robert M. Lust |
| author_facet | Elizabeth Granier Elizabeth Granier Madaniah O. Zakari Madaniah O. Zakari Musaad B. Alsahly Musaad B. Alsahly Lauren G. Koch Steven Britton Laxmansa C. Katwa Robert M. Lust Robert M. Lust |
| author_sort | Elizabeth Granier |
| collection | DOAJ |
| description | Introduction: In this study, we determined the influence of intrinsic exercise capacity on the vascular adaptive responses to hind limb ischemia. High Capacity Running, HCR; Low Capacity Running, LCR, rats were used to assess intrinsic aerobic capacity effects on adaptive responses to ischemia.Methods: Muscle samples from both ischemic and non-ischemic limb in both strains were compared, histologically for the muscle-capillary relationship, and functionally using microspheres to track blood flow and muscle stimulation to test fatigability. PCR was used to identify the differences in gene expression between the phenotypes following occlusive ischemia.Results: Prior to ligation, there were not significant differences between the phenotypes in the exhaustion time with high frequency pacing. Following ligation, LCR decreased significantly in the exhaustion time compare with HCRs (437 ± 47 vs. 824 ± 56, p < 0.001). The immediate decrease in flow was significantly more severe in LCRs than HCRs (52.5 vs. 37.8%, p < 0.001). VEGF, eNOS, and ANG2 (but not ANG1) gene expression were decreased in LCRs vs. HCRs before occlusion, and increased significantly in LCRs 14D after occlusion, but not in HCRs. LCR capillary density (CD) was significantly lower at all time points after occlusion (LCR 7D = 564.76 ± 40.5, LCR 14D = 507.48 ± 54.2, both p < 0.05 vs. HCR for respective time point). NCAF increased significantly in HCR and LCR in response to ischemia.Summary: These results suggest that LCR confers increased risk for ischemic injury and is subject to delayed and less effective adaptive response to ischemic stress. |
| first_indexed | 2024-12-21T00:13:58Z |
| format | Article |
| id | doaj.art-accf8cde6b2b486aaa07fce44942de0d |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-12-21T00:13:58Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-accf8cde6b2b486aaa07fce44942de0d2022-12-21T19:22:17ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2021-11-01810.3389/fcvm.2021.752955752955Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb IschemiaElizabeth Granier0Elizabeth Granier1Madaniah O. Zakari2Madaniah O. Zakari3Musaad B. Alsahly4Musaad B. Alsahly5Lauren G. Koch6Steven Britton7Laxmansa C. Katwa8Robert M. Lust9Robert M. Lust10Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, NC, United StatesDepartment of Biological Science, St. Louis Community College-Meremac, St. Louis, MO, United StatesDepartment of Physiology, Brody School of Medicine, East Carolina University, Greenville, NC, United StatesDepartment of Physiology, College of Medicine, Taibah University, Medina, Saudi ArabiaDepartment of Physiology, Brody School of Medicine, East Carolina University, Greenville, NC, United StatesDepartment of Physiology, College of Medicine, King Saud University, Riyadh, Saudi ArabiaDepartment of Physiology and Pharmacology, University of Toledo, Toledo, OH, United StatesDepartments of Anesthesiology and Molecular and Integrative Medicine, University of Michigan, Ann Arbor, MI, United StatesDepartment of Physiology, Brody School of Medicine, East Carolina University, Greenville, NC, United StatesDepartment of Physiology, Brody School of Medicine, East Carolina University, Greenville, NC, United StatesEast Carolina Diabetes and Obesity Center, East Carolina University, Greenville, NC, United StatesIntroduction: In this study, we determined the influence of intrinsic exercise capacity on the vascular adaptive responses to hind limb ischemia. High Capacity Running, HCR; Low Capacity Running, LCR, rats were used to assess intrinsic aerobic capacity effects on adaptive responses to ischemia.Methods: Muscle samples from both ischemic and non-ischemic limb in both strains were compared, histologically for the muscle-capillary relationship, and functionally using microspheres to track blood flow and muscle stimulation to test fatigability. PCR was used to identify the differences in gene expression between the phenotypes following occlusive ischemia.Results: Prior to ligation, there were not significant differences between the phenotypes in the exhaustion time with high frequency pacing. Following ligation, LCR decreased significantly in the exhaustion time compare with HCRs (437 ± 47 vs. 824 ± 56, p < 0.001). The immediate decrease in flow was significantly more severe in LCRs than HCRs (52.5 vs. 37.8%, p < 0.001). VEGF, eNOS, and ANG2 (but not ANG1) gene expression were decreased in LCRs vs. HCRs before occlusion, and increased significantly in LCRs 14D after occlusion, but not in HCRs. LCR capillary density (CD) was significantly lower at all time points after occlusion (LCR 7D = 564.76 ± 40.5, LCR 14D = 507.48 ± 54.2, both p < 0.05 vs. HCR for respective time point). NCAF increased significantly in HCR and LCR in response to ischemia.Summary: These results suggest that LCR confers increased risk for ischemic injury and is subject to delayed and less effective adaptive response to ischemic stress.https://www.frontiersin.org/articles/10.3389/fcvm.2021.752955/fullvascular occlusionaerobic running capacityexerciseperipheral artery occlusionlimb ischemia |
| spellingShingle | Elizabeth Granier Elizabeth Granier Madaniah O. Zakari Madaniah O. Zakari Musaad B. Alsahly Musaad B. Alsahly Lauren G. Koch Steven Britton Laxmansa C. Katwa Robert M. Lust Robert M. Lust Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia Frontiers in Cardiovascular Medicine vascular occlusion aerobic running capacity exercise peripheral artery occlusion limb ischemia |
| title | Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia |
| title_full | Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia |
| title_fullStr | Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia |
| title_full_unstemmed | Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia |
| title_short | Low Intrinsic Aerobic Capacity Limits Recovery Response to Hindlimb Ischemia |
| title_sort | low intrinsic aerobic capacity limits recovery response to hindlimb ischemia |
| topic | vascular occlusion aerobic running capacity exercise peripheral artery occlusion limb ischemia |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2021.752955/full |
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