Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus
Catharanthus roseus (C. roseus) plants were used to investigate the terpenoid indole alkaloids (TIAs) accumulation under the condition of PEG-induced drought stress. Multivariate analysis showed that 35% PEG6000 could induce more obvious and stable accumulation on proline (PRO) content and the relat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Journal of Plant Interactions |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/17429145.2017.1293852 |
| _version_ | 1818249613303349248 |
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| author | Ying Liu Qinghuan Meng Xihua Duan Zhonghua Zhang Dewen Li |
| author_facet | Ying Liu Qinghuan Meng Xihua Duan Zhonghua Zhang Dewen Li |
| author_sort | Ying Liu |
| collection | DOAJ |
| description | Catharanthus roseus (C. roseus) plants were used to investigate the terpenoid indole alkaloids (TIAs) accumulation under the condition of PEG-induced drought stress. Multivariate analysis showed that 35% PEG6000 could induce more obvious and stable accumulation on proline (PRO) content and the relative water content (RWC). The results indicated that there were gradual increase and then decrease (p < .05) in the contents of vindoline (VIN) and catharanthine (CAT) under 35% PEG6000 stress, but the content of vinblastine (VBL) increased gradually. In addition, the expression levels of tryptophan decarboxylase (TDC), strictosidine synthase (STR) and deacetylvindoline-4-O-acetyltransferase (DAT) were upregulated in plants under 35% PEG6000 stress. Further correlation analysis indicated that CAT accumulation was significantly correlated with TDC gene expression, and VBL accumulation was significantly correlated with peroxidase (p < .05). Our results suggest that the cultivation of C. roseus in drought stress would serve as effective treatment for accumulating TIAs. |
| first_indexed | 2024-12-12T15:39:17Z |
| format | Article |
| id | doaj.art-acd18e0856bd460bae7b6f8b6dc05123 |
| institution | Directory Open Access Journal |
| issn | 1742-9145 1742-9153 |
| language | English |
| last_indexed | 2024-12-12T15:39:17Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj.art-acd18e0856bd460bae7b6f8b6dc051232022-12-22T00:19:56ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532017-01-01121879110.1080/17429145.2017.12938521293852Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseusYing Liu0Qinghuan Meng1Xihua Duan2Zhonghua Zhang3Dewen Li4Ministry of Education, Northeast Forestry UniversityMinistry of Education, Northeast Forestry UniversityMinistry of Education, Northeast Forestry UniversityMinistry of Education, Northeast Forestry UniversityMinistry of Education, Northeast Forestry UniversityCatharanthus roseus (C. roseus) plants were used to investigate the terpenoid indole alkaloids (TIAs) accumulation under the condition of PEG-induced drought stress. Multivariate analysis showed that 35% PEG6000 could induce more obvious and stable accumulation on proline (PRO) content and the relative water content (RWC). The results indicated that there were gradual increase and then decrease (p < .05) in the contents of vindoline (VIN) and catharanthine (CAT) under 35% PEG6000 stress, but the content of vinblastine (VBL) increased gradually. In addition, the expression levels of tryptophan decarboxylase (TDC), strictosidine synthase (STR) and deacetylvindoline-4-O-acetyltransferase (DAT) were upregulated in plants under 35% PEG6000 stress. Further correlation analysis indicated that CAT accumulation was significantly correlated with TDC gene expression, and VBL accumulation was significantly correlated with peroxidase (p < .05). Our results suggest that the cultivation of C. roseus in drought stress would serve as effective treatment for accumulating TIAs.http://dx.doi.org/10.1080/17429145.2017.1293852Catharanthus roseusalkaloidsPEGvindolinecatharanthinevinblastine |
| spellingShingle | Ying Liu Qinghuan Meng Xihua Duan Zhonghua Zhang Dewen Li Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus Journal of Plant Interactions Catharanthus roseus alkaloids PEG vindoline catharanthine vinblastine |
| title | Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus |
| title_full | Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus |
| title_fullStr | Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus |
| title_full_unstemmed | Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus |
| title_short | Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus |
| title_sort | effects of peg induced drought stress on regulation of indole alkaloid biosynthesis in catharanthus roseus |
| topic | Catharanthus roseus alkaloids PEG vindoline catharanthine vinblastine |
| url | http://dx.doi.org/10.1080/17429145.2017.1293852 |
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