Cap ‘n’ Collar C and Aryl Hydrocarbon Receptor Nuclear Translocator Facilitate the Expression of Glutathione S-Transferases Conferring Adaptation to Tannic Acid and Quercetin in <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae)
<i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) is a notorious pest of poplar. Coevolution with poplars rich in plant secondary metabolites prompts <i>M. troglodyta</i> to expand effective detoxification mechanisms against toxic plant secondary metaboli...
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2190 |
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| author | Zhiqiang Wang Xiaoling Shi Yujingyun Zhou Fang Tang Xiwu Gao Pei Liang |
| author_facet | Zhiqiang Wang Xiaoling Shi Yujingyun Zhou Fang Tang Xiwu Gao Pei Liang |
| author_sort | Zhiqiang Wang |
| collection | DOAJ |
| description | <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) is a notorious pest of poplar. Coevolution with poplars rich in plant secondary metabolites prompts <i>M. troglodyta</i> to expand effective detoxification mechanisms against toxic plant secondary metabolites. Although glutathione S-transferases (GSTs) play an important role in xenobiotic detoxification in <i>M. troglodyta</i>, it is unclear how GSTs act in response to toxic secondary metabolites in poplar. In this study, five <i>GST</i> gene core promoters were accurately identified by a 5’ loss luciferase reporter assay, and the core promoters were significantly induced by two plant secondary metabolites in vitro. Two transcription factors, cap ‘n’ collar C (CncC) and aryl hydrocarbon receptor nuclear translocator (ARNT), were cloned in <i>M. troglodyta</i>. MtCncC and MtARNT clustered well with other insect CncCs and ARNTs, respectively. In addition, MtCncC and MtARNT could bind the <i>MtGSTt1</i> promoter and strongly improve transcriptional activity, respectively. However, MtCncC and MtARNT had no regulatory function on the <i>MtGSTz1</i> promoter. Our findings revealed the molecular mechanisms of the transcription factors MtCncC and MtARNT in regulating the <i>GST</i> genes of <i>M. troglodyta</i>. These results provide useful information for the control of <i>M. troglodyta</i>. |
| first_indexed | 2024-03-11T09:42:07Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:42:07Z |
| publishDate | 2023-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-acd21804b1fc4d7f930bc22091ae9a9d2023-11-16T16:53:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243219010.3390/ijms24032190Cap ‘n’ Collar C and Aryl Hydrocarbon Receptor Nuclear Translocator Facilitate the Expression of Glutathione S-Transferases Conferring Adaptation to Tannic Acid and Quercetin in <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae)Zhiqiang Wang0Xiaoling Shi1Yujingyun Zhou2Fang Tang3Xiwu Gao4Pei Liang5Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, China<i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) is a notorious pest of poplar. Coevolution with poplars rich in plant secondary metabolites prompts <i>M. troglodyta</i> to expand effective detoxification mechanisms against toxic plant secondary metabolites. Although glutathione S-transferases (GSTs) play an important role in xenobiotic detoxification in <i>M. troglodyta</i>, it is unclear how GSTs act in response to toxic secondary metabolites in poplar. In this study, five <i>GST</i> gene core promoters were accurately identified by a 5’ loss luciferase reporter assay, and the core promoters were significantly induced by two plant secondary metabolites in vitro. Two transcription factors, cap ‘n’ collar C (CncC) and aryl hydrocarbon receptor nuclear translocator (ARNT), were cloned in <i>M. troglodyta</i>. MtCncC and MtARNT clustered well with other insect CncCs and ARNTs, respectively. In addition, MtCncC and MtARNT could bind the <i>MtGSTt1</i> promoter and strongly improve transcriptional activity, respectively. However, MtCncC and MtARNT had no regulatory function on the <i>MtGSTz1</i> promoter. Our findings revealed the molecular mechanisms of the transcription factors MtCncC and MtARNT in regulating the <i>GST</i> genes of <i>M. troglodyta</i>. These results provide useful information for the control of <i>M. troglodyta</i>.https://www.mdpi.com/1422-0067/24/3/2190<i>Micromelalopha troglodyta</i> (Graeser)transcription factorsdetoxification genespromotersplant secondary metabolites |
| spellingShingle | Zhiqiang Wang Xiaoling Shi Yujingyun Zhou Fang Tang Xiwu Gao Pei Liang Cap ‘n’ Collar C and Aryl Hydrocarbon Receptor Nuclear Translocator Facilitate the Expression of Glutathione S-Transferases Conferring Adaptation to Tannic Acid and Quercetin in <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) International Journal of Molecular Sciences <i>Micromelalopha troglodyta</i> (Graeser) transcription factors detoxification genes promoters plant secondary metabolites |
| title | Cap ‘n’ Collar C and Aryl Hydrocarbon Receptor Nuclear Translocator Facilitate the Expression of Glutathione S-Transferases Conferring Adaptation to Tannic Acid and Quercetin in <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) |
| title_full | Cap ‘n’ Collar C and Aryl Hydrocarbon Receptor Nuclear Translocator Facilitate the Expression of Glutathione S-Transferases Conferring Adaptation to Tannic Acid and Quercetin in <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) |
| title_fullStr | Cap ‘n’ Collar C and Aryl Hydrocarbon Receptor Nuclear Translocator Facilitate the Expression of Glutathione S-Transferases Conferring Adaptation to Tannic Acid and Quercetin in <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) |
| title_full_unstemmed | Cap ‘n’ Collar C and Aryl Hydrocarbon Receptor Nuclear Translocator Facilitate the Expression of Glutathione S-Transferases Conferring Adaptation to Tannic Acid and Quercetin in <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) |
| title_short | Cap ‘n’ Collar C and Aryl Hydrocarbon Receptor Nuclear Translocator Facilitate the Expression of Glutathione S-Transferases Conferring Adaptation to Tannic Acid and Quercetin in <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) |
| title_sort | cap n collar c and aryl hydrocarbon receptor nuclear translocator facilitate the expression of glutathione s transferases conferring adaptation to tannic acid and quercetin in i micromelalopha troglodyta i graeser lepidoptera notodontidae |
| topic | <i>Micromelalopha troglodyta</i> (Graeser) transcription factors detoxification genes promoters plant secondary metabolites |
| url | https://www.mdpi.com/1422-0067/24/3/2190 |
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