Genome–Wide Identification and Functional Characterization of Auxin Response Factor (ARF) Genes in Eggplant
Auxin response factors (ARFs) are important plant transcription factors that are differentially expressed in response to auxin and various abiotic stresses. ARFs play important roles in mediating plant growth and stress responses; however, these factors have not been studied in eggplants. In this st...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/11/6219 |
| Summary: | Auxin response factors (ARFs) are important plant transcription factors that are differentially expressed in response to auxin and various abiotic stresses. ARFs play important roles in mediating plant growth and stress responses; however, these factors have not been studied in eggplants. In this study, genome–wide identification and the functional analysis of the ARF gene family in eggplants (<i>Solanum melongena</i> L.) were performed. A total of 20 <i>ARF</i> (<i>SmARF</i>) genes were identified and phylogenetically classified into three groups. Our analysis revealed four functional domains and 10 motifs in these proteins. Subcellular localization showed that the SmARFs localized in the nucleus. To investigate the biological functions of the <i>SmARFs</i> under 2,4–D and salt stress treatments, quantitative real–time RT–PCR (qRT–PCR) was conducted. Most <i>SmARF</i> genes exhibited changes in expression in response to 2,4–D treatments in the flowers, especially <i>SmARF4</i> and <i>7B</i>. All <i>SmARF</i> genes quickly responded to salt stress, except <i>SmARF17</i> and <i>19</i> in leaves, <i>SmARF1A</i> and <i>7B</i> in roots, and <i>SmARF2A</i>, <i>SmARF7B</i>, and <i>SmARF16B</i> in stems. These results helped to elucidate the role of ARFs in auxin signaling under 2,4–D and salt stress in eggplants. |
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| ISSN: | 1661-6596 1422-0067 |